3.6.5. Southern hybridization Southern blot hybridization analysis was carried out using standard procedures (Sambrook et al. 2001). Approximately 20 µg of total genomic DNA from transgenic plants and untransformed control plant was digested with EcoRI‚ and then separated by electrophoresis on 0.8% agarose gel. Digestion profile was checked with 1µg DNA‚ with single cutter enzymes EcoRI (10U) in T-DNA region. For southern experiment‚ 20µg DNA was digested overnight. Restricted fragments were separated
Premium Molecular biology Western blot Southern blot
Gulf Oil Corporation in 1984. This merger was the largest merger till that time in the history of the United States and it doubled the oil and gas reserves of the company. Chevron merged with Texaco in 2001 & formed a new company named ChevronTexaco. Texaco was one of the branches of Chevron family. It was formed in 1901 in Beaumont‚ Texas. To convey a unified presence in the world‚ it was again renamed to Chevron in 2005. Chevron strengthened its position by acquiring Unocal Corporation in 2005.
Premium Chevron Corporation Petroleum
south is known for a lot of things‚ amazing sweet tea‚ good barbeque‚ but have you ever thought about southern hospitality? No‚ it isn’t one of Ludacris’s albums‚ well‚ not in this sense anyway. Southern Hospitality to me is defined as people being willing enough to volunteer their time‚ homes‚ churches‚ etc to the community‚ or even country. Tennessee in particular is well known for their southern hospitality‚ and willingness to volunteer. We’re called the Volunteer State because in the War of 1812
Premium Southern United States United States Native Americans in the United States
In the fifth chapter‚ this thesis will assess the abolitionist effort to denounce the legitimacy of using the Bible to sanction southern slavery by arguing that biblical slavery was not based upon the inferiority of one race whereas southern slavery was based upon the inferiority of one race. In short‚ these abolitionists sought to highlight that southerners were using a book which sanctioned a system of slavery that was not based upon the inferiority of one race to sanction a system of slavery that
Premium Slavery in the United States Slavery American Civil War
Mr. President: How and Why the Founders Created a Chief Executive Analysis On June 1‚ 1787 Federal Convention first talked of establishing a new executive branch. James Wilson said that the Executive should consist of a single person. To U.S. citizens today‚ this is an obvious statement‚ but it wasn’t at that time. Americans had just won their independence from an autocratic monarch‚ Britain. They feared that another oppressive leader would rise and try to dictate to the newly independence-gained
Premium United States Constitution President of the United States
CASE PREPARATION CHART Student Name Student ID Submission date Case title Alza corporation: A case study concerning R&D accounting practices in the pharmaceutical industry Section ASSESSMENT To be filled by facilitator Components Scores Scores 1 mark 2 marks 3 marks 4 marks Completeness of case chart Case chart is incomplete Some of the case chart requirements are met satisfactorily. Most of the case chart requirements are met satisfactorily. All case
Premium Generally Accepted Accounting Principles Income statement Balance sheet
OneSource One-Stop Report LG Corp. 5 February 2013 OneSource One-Stop Reports for LG Corp. LG Corp. LG Twin Tower 20‚ Yeouido-Dong‚ Yeongdeungpo-Gu Seoul‚ 150721 Korea‚ Republic of Tel: Fax: 82-2-37771114 82-2-37732292 Employees: Company Type: Corporate Family: Traded: Incorporation Date: Auditor: 92 Public Parent 212 Companies Korea Stock Exchange: 05-Jan-1947 Deloitte & Touche LLP Financials in: GBP (000) 003550 Fiscal Year End: 31-Dec-2011 Reporting Currency: South Korean Won
Premium South Korea
Table of Contents 1.0 Abstract Biovail Corporation‚ a large pharmaceutical company and had applied advanced drug-delivery technologies to improve the clinical effectiveness of medicines. It is recently had its stock downgraded by a well-known pharmaceutical analyst and a number of other analysts were also scrutinizing the company. The outcome was not favorable‚ as Biovail ’s acquisition methods were labeled as unethical and their accounting practices were questioned
Premium Corporation Investment Economics
Marriott Corporation: The Cost of Capital April 2012 Executive Summary Determining the appropriate cost of capital for new investment projects for a diversified company like the Marriott Corporation is not an easy endeavor. However‚ it is an important exercise because the more effective the process‚ the better it can help to support the company’s growth objective with its financial strategy. The four components of the financial strategy are: manage rather than own hotel
Premium Investment Finance Weighted average cost of capital
APPENDIX A Questionnaire Republic of the Philippines SOUTHERN LUZON STATE UNIVERSITY Gumaca Campus Gumaca‚ Quezon Software Acceptability and Features | Rating | | 1 | 2 | 3 | 4 | A. Functionality of the Propose System | | | | | 1. The system can accept organized data | | | | | 2. The system has the ability to be used by the admin‚ manager and user | | | | | 3. The system operates in real time | | | | | 4. The system prevents unauthorized person
Free Philippines Luzon Computer software