DENATURATION OF PROTEINS Abstract The experiment was done to be able to understand how various denaturants such as HCl and NaOH affects proteins. It was observed that different denaturants act upon or denature protein differently. This was determined using the principle of viscometry. An Ostwald viscometer was used to measure the viscosity of the prepared native‚ blank‚ denatured native and blank with denaturant solutions. The time required for the said solutions to pass through the viscometer
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LAB TH 7-9:50pm 29 August 2013 Biology Lab Report Lab #1 –PROTEIN EXTRACTION LAB I. INTRODUCTION To begin the process of protein extraction and compare the results in a study‚ it is necessary to understand the importance of proteins‚ the process of extraction and how you are using the results to determine a rational conclusion. First understand proteins and the necessity of studying their impact. Proteins are essential molecules for biological functions and are the stimulant for
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Denaturing of Proteins Research Paper Abstract: Introduction The experiments the objective is to determine‚ the possible measures of the denaturation process of several proteins by using a home based temperate The necessary applications of this experiment are to compile all the possible measures of the denaturation process in understandable units and explainable means. Other methods possible for research are by using a
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of solutions and test their pH. Due to materials available pH strips were the only testing tools available. A table is setup to compare the strip color to its pH level. Four known solutions including bleach‚ lemon juice‚ soda‚ and baking soda were used. There was also an unknown solution in which the pH level was used to determine which solution it was. The pH scale is 1-14‚ a pH of 7 is neutral‚ less than 7 is acidic‚ and greater than 7 is acidic. Materials: * pH strips * 20 test tubes
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Practical 3: Proteins Introduction: ‘Protein’ also an important food component‚ we commonly take in from our diet. In this experiment we have studied a certain property of protein‚ i.e.‚ protein can be denatured by various factors. A. Denaturation of egg white Results: Egg white sample in water baths | Test tube | temperature | Observations | 1 | 60°C | Took 7minutes and 15 seconds to turn white and form white participate. | 2 | 80°C | Took 2minutes and 20 seconds to turn white
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4A.2 RRL 4A.2.1 Coagulation of Proteins Coagulation of protein refers to sticking together‚ like a blood clot‚ usually as a result of denaturation or coming out of solution due to abnormal ionic strength or a change of solvent. Definite characteristics of the proteins are changed when they are coagulated‚ among which is loss of solubility in water and dilute salt solutions. In some instances and under certain conditions the coagulation process may be reversible. (Campbell‚ et.al‚ 1979) 4A.2.2
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Lab 2: Internet Resources for Protein Biochemistry Part 1-1: A) Protein Accession Number = P00939 Name of Protein: Full Name = Triosephosphate isomerase; Short Name=TIM Organism where the protein is found: Oryctolagus cuniculus = Rabbit Nuber of amino acids in the protein: AA = 248 B) * Amino Acid Sequence: >gi|136066|sp|P00939.1|TPIS_RABIT RecName: Full=Triosephosphate isomerase; Short=TIM;
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Protein Monique Rowland Nutrition / 241 Instructor: AN-CHIAN CHEN October 4‚ 2013 Protein All proteins are molecules that are the boosters of living organisms and are part of each organ‚ tissue‚ and cell within the human body. The protein in consumed food turns into amino acid. The amino acid is used later replinish the proteins used. Proteins have two structures. the first and primary structure of protein is the sequence of the amino acid in the protein (Kim‚ 2005). The amino acid
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Importance of maintaining a clean living environment and following orders. It is important to maintain a clean and sanitary living environment for multiple reasons. One of the reasons is that it is a threat to public health in the barracks if even just one of the soldiers is living in unsanitary conditions‚ then through frequent contact throughout the day with other soldiers that one soldier‚ if he or she becomes sick‚ could cause other soldiers to become sick. When one soldier becomes the source
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exchange component to be taken account of. Equally‚ however‚ a notional amount of principal is required in order to compute the actual cash amounts that will be periodically exchanged. FEATURES: Interest Rate Swap Situation A bank needs to balance its floating interest rate portfolio with more fixed rate loans‚ so it contacts a company who has a high percentage of fixed rate loans and offers to do an interest rate swap. Interest Rate Swap Terms The two companies negotiate and‚ depending
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