Objective: To investigates the enzymatic effect of various materials in the hydrogen peroxide solution. Apparatus & Equipment: Beaker Test tubes Either: water bath (95oC) or Bunsen burner Materials: Fresh Liver Potato cubes Manganese dioxide Hydrogen peroxide** Wood splints **Caution: Hydrogen peroxide is formed continuously as a by-product of chemical reactions in living cells; it is a very toxic (poisonous) substance. [Note: using boiling tubes may provide
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Determination of the Enthalpy for Decomposition of Hydrogen Peroxide Objective: To construct a coffee cup calorimeter‚ measure its calorimeter constant‚ and determine the enthalpy of decomposition and formation of hydrogen peroxide. Background: This experiment is a classic thermodynamics lab. In it‚ we attempt to measure the enthalpy (H) of a chemical reaction. The main obstacle is that this is a quantity that cannot be measured directly. It instead is observed as heat from one substance is transferred
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This experiment shows the effects of changing the pH level has on Catalase. As predicted‚ the farther away the pH levels got from the optimum pH (7.2)‚ the lower the reaction rate. At a pH of 7.2‚ the foam of the reaction measures 6cm. At a ph of 3 it measures 2.5 cm‚ at a pH of 5 it measures 2.75 cm‚ at ph 9 it measures 2.3 and at 11cm it measures 2cm. pH measures the hydrogen ion concentration of a substrate. By changing the pH of the catalase‚ the enzyme was denatured. Denaturing is the result
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stage while the larvae needs brackish water and usually found in estuaries (Galitzine and Morgan‚ 2009). This species of prawn is a tropical living animal which needs warmer temperatures more than 18°C for growth process. (Dasgupta‚ 2005). However‚ for culture process‚ the optimal production of the prawns needs minimum temperature at 82.4°F (New B. ‚ 2002). In aquaculture‚ the four highest producers of the genus Macrobrachium were China‚ Thailand‚ India and Bangladesh in 2007 (FAO‚ 2009). In 2007‚ the
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determine the speed at which a reaction took place between an iodine and hydrogen peroxide solution. In addition to a change in concentration‚ a change in temperature and a catalyst variable was also introduced to conclude whether or not their presence affected the overall speed of the reaction. In order to determine the effects of these variables‚ several iodine and hydrogen peroxide reactions were prepared‚ (all at varying temperatures‚ volumes‚ and concentrations)‚ and reacted until the solutions color
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"To See What Factors Affect The Decomposition Of Hydrogen Peroxide By The Enzyme Catalase Which Is Found In The Liver" Introduction: Enzymes are biological catalysts. They speed up the chemical reactions which go on inside living things. Without them the reactions would be so slow that life would grind to a halt. Enzymes work by when a substrate molecule bumps into a molecule of the right enzyme‚ it fits into a depression on the surface of the enzyme molecule. This depression is called the
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INVESTIGATION INTO THE EFFECT OF TEMPERATURE ON CATALASE ACTIVITY AIM The aim of this experiment is to find out the effect of temperature on catalase or hydrogen peroxide. This will enable us to tell at what temperature hydrogen peroxide is most efficient. This (degradation) reaction will help us determine some of the catalase’s different attributes. HYPOTHESIS In this experiment it would be safe to hypothesise that no activity would take place at 1 to 20 degrees. It would be probable that a
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Research Question Does the concentration of substrate (H2O2 (hydrogen peroxide)) have an effect on the activity of the enzyme (catalase)? Theory The higher the substrate concentration the more quickly product is produced (rate of reaction increases) until enzyme saturation is reached at which time more substrate has no further effect. Enzymes such as Catalase are protein molecules which are found in living cells. They are used to speed up specific reactions in the cells. They are all
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THE EFFECT OF PH ON THE ACTIVITY OF CATALASE Aim : To study the effect of pH on the activity of catalase. Introduction : Catalase‚ an enzyme found in many different tissues‚ catalyses the breakdown of hydrogen peroxide into water and oxygen. 2 H202 → 2H20 + O2 Hydrogen peroxide is a toxic substance that can be formed during aerobic respiration and catalase removes this product. The activity of catalase can be measured by finding the rate of oxygen release from hydrogen peroxide. Potato
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For many years Hydrogen peroxide was widely used for disinfecting‚ sterilization and its antiseptic properties‚ it is cheap and easily available OTC at any drug store or grocery store. “This readily available oxidant is rapidly converted to the highly reactive hydroxyl radical that damages an array of cellular components” (Atiyeh‚ Dibo‚ and Hayek‚ 2009). “The Medicines and Healthcare Products Regulatory Agency (MHRA‚ 2014). Have issued a warning regarding its use in surgery and in deep and large
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