3.7.2.1.5 Urea test PRINCIPLE Urea is hydrolyzed in the presence of urease to produce ammonia and CO2. The ammonia produced combines with 2 – oxoglutarate and NADH in the presence of GLDH to yield glutamate and NAD. Urea + H2O + 2H+ 2NH4+ + CO2 NH4+ + 2-Oxoglutarate +NADH H2O +NAD+ + Glutamate The decrease in absorbance due to the decrease of NADH concentration in unit time is proportional to the urea concentration. Ammonia
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Zebrafish Embryotoxicity Test (ZET) The ZET will be done following the method proposed by Beekhuijzen et al. (2015) with modifications. Six embryos will be exposed to each of the following treatments: 0.1‚ 0.05‚ 0.025‚ 0.0125‚ and 0.00625 mg/ml. Embryos will be incubated in 24-well plates with built-in lids. Each well will contain one embryo and 2 ml of the respective treatment. All test solutions will be renewed every 24h to maintain the integrity of the test concentrations. The embryos will
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Description for Using Malaria Rapid Diagnostic Test (mRDTs) The procedure of the test as contained in the manufacturer’s instructions as approved by the National guidelines for diagnosis and treatment of malaria‚ (FMoH‚ 2011) will be followed. its reliability was futher proven by Olaniyan‚ et al.‚ (2013). The RDT provided for the study has LOT number and expiry date which must be recorded. Preferably RDT of known quality assurance will be used. The device usually contains a cassette‚ an applicator
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As a result of the tests ran‚ I was able to identify the unknown bacteria. For Unknown A‚ I ran six tests. I first isolated the bacterium from the second bacterium and found a clear growth (Table 1‚ Figure 1). Secondly‚ I ran a gram stain and found a gram positive‚ cocci bacterium (Table 1‚ Figure 2). Third‚ I ran a catalase test in which was negative (Table 1). From here‚ I determined a starch hydrolysis test would be necessary to distinguish between different bacteria. The result was negative (Table
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10 Test Tube Mystery Introduction: The students were handed 10 test tubes with no identification. The students were supposed to design a method in determining what chemical was in each test tube. The list of chemicals is CuSO4‚ NH4Cl‚ NaOH‚ AgNO3‚ KI‚ H2SO4‚ NaBr‚ CaCl2‚ HCl‚ and Pb(NO3)2. The students are expected to determine the chemicals using physical properties‚ litmus paper‚ solubility‚ and the process of elimination. Physical properties‚ such as the color‚ can be determined by sight. Litmus
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1. Both of the subjects in this test preformed excellent compared to the rankings provided. Victors Mean Power put him in between the 85 to 90 percentile with his score of 639.83 and his Anaerobic Capacity put him in between the 60 to 65 percentile. These scores indicate that Victor trains more with resistance than aerobic type exercises because of the high score of his Mean Power. These scores also correlate with his VO2 Max test we preformed earlier. His VO2 Max of 3.4 is low compared to the high
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Detecting ECG Daniela Paszkiewicz and Lyndsey Wessel Abnormalities in VO2 Max Tests ECG “An electrocardiogram (ECG) is a medical test that detects cardiac (heart) abnormalities by measuring the electrical activity generated by the heart as it contracts.” http://www.istockphoto.com/file_thumbview_approve/3733 698/2/istockphoto_3733698-electrocardiogram-ecg-ekgwith-human-heart-on-screen.jpg Function of Electrocardiograph Electrocardiograph measures a patients ECG ECG can help detect a
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1. Introduction The Jominy end-quench test is a technique that has the ability to harden the steel by quenching. It is an essential feature of materials that determine the material’s selection for particular applications. The different types of steels and alloys that are used for hardening in an industry are done by this test and considering that this test is a standardised experiment [1]. 1.1 Aim The specimens that are used in this test are normalized in accordance with the standards. The purpose
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load test Aim: To determine the strength characteristics of a rock using the point load test and visual judgement. Materials Loading device Loading frame Pump Ram Platens Rock Caliper Method (Standard‚ 2007) 1. Measure the dimensions of the rock which includes the diameter and length 2. Find the length to diameter ratio to determine which test will be used. If the ratio is greater than 1 use diametrical test. But if the ratio is between 0.6 and 1 use axial test 3. The axial test was
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3. LABORATORY TESTS USED TO STUDY THE STRENGTH OF CALCARENITES There are 3 distinct methods or tests used to determine the strength of calcarenite which are needle penetration test‚ unconfined compression test‚ and direct shear box test. 3.1. Needle penetration test This form of test is done on site on an outcrop rock that is not disturbed; this is usually done by extracting a sample material for oncoming laboratory work. The penetration tests are done on two different sections of outcrop with an
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