The Effect of Temperature on Enzyme Activity and Oxygen Production Throughout this report you will gain information as to how temperature effects the amount of oxygen produced in an enzyme- catalase experiment. In the experiment we used liver extract as a catalase and created a chemical reaction within a reaction chamber between the catalase and hydrogen peroxide as well as three different controlled temperatures. In the procedure below there will be a step by step process as to how
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of the Enzyme Catalase in breaking down Hydrogen Peroxide and the effect of various factors on Enzyme Activity Introduction The enzyme catalase is present in cells in order to speed the breakdown of hydrogen peroxide (H2O2)‚ which is a toxic chemical to the human body. When hydrogen peroxide is broken down‚ the end products are Water (H2O) and Oxygen (O2). In this report‚ the reaction of catalase to hydrogen peroxide is being tested. Furthermore‚ the effects of temperature‚ concentration
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Effect of Temperature on Enzyme Activity Bernard Stepteau Biology Lab 101/Th 8:00am – 9:50am 2-26-2014 Dr Laynes Hypothesis: As the temperature of the enzyme catalase rises the activity of the reaction will decrease. Objectives: The objective is to compare catalase activity at different temperatures. Introduction Catalase speeds the breakdown of hydrogen peroxide. Catalase is present in every plant and animal organ. This is useful because hydrogen peroxide is harmful
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substrate concentration‚ and the effect of temperature on the rate of Enzyme-Catalase reaction. In experiment one (i.e. the effect of varying substrate concentration on the rate of enzyme-catalase reaction) we tested the hypothesis HA as substrate concentration increases‚ so will reaction rate until all active sites are bound. In experiment two (i.e. The effect of temperature on the rate of enzyme-catalase reaction) we tested the hypothesis HA as temperature increases‚ so will
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into the second beaker containing the potato catalase for exactly 5 seconds. It was next dried on a paper towel for 5 seconds‚ and finally placed at the bottom of the first beaker‚ which contained hydrogen peroxide solution. The timer was then started. The timer stopped when the paper disk floated up to the top of the beaker. This was performed 3 times. After this‚ two negative controls were tested. The first negative control used a denatured catalase instead of the potato extract. The
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Catalase Lab Introduction: Catalase is an enzyme normally found in many plant and animal tissues. Its purpose is to destroy toxic substances which may be introduced into cells. Also‚ some cells use catalase to destroy cellular debris or worn out organelles. In this lab‚ we will use a catalase solution from potatoes and determine the effect of temperature and pH on the action of this enzyme. The substrate of the enzyme will be 3% hydrogen peroxide (H2O2). Catalase works by the following mechanism:
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Simple Experiments on the Enzyme Catalase Aim: The aim of this practical is to use three different techniques to investigate the effect of different concentrations of the enzyme catalase on the rate of breakdown of hydrogen peroxide. Background information Catalase is an enzyme which is found in all living organisms. This enzyme catalases the decomposition of hydrogen peroxide into water and oxygen. Cells continually produce a poisonous by-product of metabolising‚ called hydrogen peroxide. This
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Abstract: Enzymes‚ molecules that speed up chemical reactions‚ are specific to one substrate. In this experiment the substrate hydrogen peroxide and the enzyme catalase will be used. The higher the concentration of potato extract‚ or catalase‚ the faster the reaction and the more substrate present will result in a decrease in the time of the reaction. The amount of concentrations of enzymes and substrates are changed to determine if the reaction is further catalyzed by a greater concentration of
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Substrate concentration and yeast catalase Aim: To see how the substrate concentration in hydrogen peroxide affects the rate of an enzyme controlled reaction using yeast catalase. Introduction: An enzyme is a biological catalyst made of protein. Enzymes are protein molecules found in living organisms and in this case I will use a yeast catalase. Catalase is an enzyme that catalyzes the reduction of hydrogen peroxide. Hydrogen peroxide is a poisonous by-product of metabolism‚ so it is very
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Abstract The following experiment details the effect of different concentrations of catalase on the production of oxygen and water through the breakdown of Hydrogen Peroxide. In this experiment paper disc where coated in varying concentrations of catalase‚ 0‚ 25‚ 50 75 and 100%. The time taken for the disc to float between two markers on the side of a glass was then recorded. This experiment demonstrates that the higher the concentration of enzyme used the greater the production of oxygen on the
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