Light Microscopy Lab Lab write-up 1 Parveen Rajper Biology 101 Principles of Biology 1 September 29‚ 2012 Introduction: The light microscope helps see the microscopic world that the naked eye cannot see. If there were no microscopes in the world‚ than it would be very difficult to understand the many particles that surround the living world. The purpose of this lab experiment is to become familiar with a microscope. It is hypothesized that it will be difficult
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Laboratory Report: Observing Bacteria Purpose: Is to gain knowledge on how to use a microscope and oil immersion lens while observing prepared bacterial slides and also to prepare slides for observable bacteria culture from yogart. Procedure: Exercise 1: Using the Microscope - Viewing Prepared Slides I started out by reading and reviewed the science lab safety reinforcement Agreement and the instruction to oil immersion lens. After setting up my lab station I view the six prepared slides
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Definition of the parts of the microscope 1. Stage The horizontal surface upon which the slide is placed is called the stage. The slide is held in place by spring loaded clips and moved around the stage by turning the geared knobs on the stage. Th stage has two perpendicular scales that can be used to record the position of an object on a slide. This is useful if you want to quickly relocate an object. 2. Coarse adjustment The coarse adjustment knob located on the arm of the microscope
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1) The condenser lens concentrates light and makes illumination of image more uniform. 2) The bending of light is also known as refraction. 3) Total magnification = ocular lens magnification x objective lens magnification. 4) The total magnification of an image is 1200x and the magnification of the ocular lens is 10x. What is the magnification of the objective lens? Y = objective lens 1200x = 10x X y 1200x / 10x = y y = 120x Objective lens magnification = 120x 5) The limit of resolution
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BIO Microscopy‚ Mitosis‚ and Motility Lab Discussion Questions In the Microscopy‚ Mitosis‚ and Motility lab‚ the class used the Compound Microscope. The Compound Microscope helped examine the prokaryotic and eukaryotic cells‚ observe the different stages of mitosis‚ and compare modes of motility used by protists. The light compound microscope works with the aid of lenses such as the eyepiece. These lenses help keep the focus of the light on the eye. The fine and course adjustments also assist with
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BIOLOGY 181 Lab # 4 Microscopy and Cells Lab A microscope is a tool that enables us to see objects and organisms that are invisible to the naked eye. Microscopes use a series of two or more lenses to magnify an illuminated image. Magnification is a measure of how big an object looks to your eye compared to “life size.” Microscopes also enhance the resolution of an image. Resolution is the ability to distinguish between two objects that are close together. Resolution can be increased using immersion
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PRACTICAL 3 QUESTIONS PART 1 Q 1. What is the diameter of the field of view of your microscope using the x10 objective (i.e. at a magnification of x100)? The diameter of the field of view using the x10 objective is 1800 µm. Q 2. What is the diameter of the field of view of your microscope using the x40 objective (i.e. at a magnification of x400)? The diameter of the field of view using the x40 objective is 400 µm. Q 3. What is the ratio of magnification between the x10 and x40 objectives?
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Exercise No. 1 Micrometry: The Science of Standardized and Calibrated Micrometric Measurements (The Jellybeans) Joaquim B. Aganon‚ Glaiza S. Ibanez (14 August 2014) (21 August 2014) I. Introduction Microscopes and the field of Science are two inseparable things. Almost all Sciences are associated with the use of a microscope‚ a device use to view objects which are not visible to the naked eye. The term microscope came from the Greek term “Mikros” which means “small” and “Skopien” which means
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Northern Caribbean University Department of Biology‚ Chemistry and Environmental Sciences **** BIOL 165: ANATOMY & PHYSIOLOGY1 Laboratory Manual 2013-2014 http://www.udel.edu/biology/Wags/histopage/colorpage/cin/cin.htm To be used in Anatomy & Physiology-BIOL 165 & 166 labs only Not to be used as a source of reference Not to be sold or distributed outside of the classroom Lecturers: Nadia Washington‚ MSc. Delano Lewis‚ PhD. Disclaimer
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Exercise 1: Microscopy Nour Bairekdar Prof. Mary Flannery September 9‚ 2013 Learning parts and proper use of microscope‚ and the interception of images viewed through a light microscope Abstract: A compound light microscope uses two sets of lenses‚ ocular and objective‚ and employs light as its source of illumination. Through careful observation‚ we identified the various parts of a light microscope we found that the light microscope Ocular lenses which give 10x magnification
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