is associated with lactase persistence is the located upstream of the LCT gene. In this mutation a single nucleotide polymorphism changes a cytosine into a thymine that then can be detected using the polymerase chain reaction technique (Biology 225 lab manual‚ S2017). In this experiment amplification Refractory Mutation System PCR was used to detect this nucleotide change. Two primers were used in two different PCR reactions‚ one to detect the wild type allele and the other to detect the mutant allele
Premium Glucose Carbohydrate Sugar
African American Studies Final Paper The first reading I chose was “A’n’t I a woman” by Soujourner Truth. Soujourner Truth was originally named Isabella Baumfree at birth. Truth was born into slavery on November 26‚ 1883 in New York where she was later freed by the New York State Emancipation Act of 1827. This was written ten years before the Civil war and at this point‚ African Americans began fighting for their freedom. “A’n’t I a Woman?” was first heard during a famous speech given at a women’s
Premium Slavery Slavery in the United States African American
discussions with my lab team. “ Signed_________________________________________ Abstract A common road salt‚ magnesium chloride‚ was analyzed in the lab to test its effectiveness as a road salt. The Van’t Hoff factor of the salt and the enthalpy of dissolution when dissolved in pure water were experimentally tested to evaluate its many characteristics as a deicer. To determine the Van’t Hoff factor‚ the difference of temperatures of freezing water and a solution of magnesium chloride was determined
Premium Enthalpy Water Sodium chloride
This analysis concluded that the unknown was ethylene glycol. This was discovered as the gas was heated to 90 degrees Celsius causing the liquid unknown to evaporate into a gas while recording the mass‚ volume‚ temperature‚ and volume. This provided the mass of 1.6 grams which was found by subtracting the mass of the Erlenmeyer flask‚ aluminum cover‚ and rubber band from the mass of the Erlenmeyer flask‚ aluminum cover and rubber band and unknown gas after heating. Then the temperature of the gas
Premium Gas Water Chemistry
Title: Prep of t-Butyl Chloride via SN1 Reaction Purpose: The purpose of this experiment is to synthesize tert-butyl chloride via an SN1 reaction. t-Butyl Chloride was synthesized from t-Butyl Alcohol using hydrochloric acid in separatory funnel; isolation of t-Butyl Chloride was done under distillation conditions. The experiment resulted in 8.29grams of purified compound‚ which is a 66.27 percent yield. Procedure: As per handout with changes Equation: Mechanism: Results: (Scan
Premium Solvent Alcohol Distillation
Introduction: In this experiment‚ when vinyl strip and acetate strip is rubbed by tissue‚ it will charge the vinyl strip negatively and acetate strip positively caused by the transfer of electron between the strip and tissue. If molecules have non zero bond dipole‚ because polar molecule must have a non-zero molecular dipole where electron spend more of their time closer to one atomic nucleus than the other and make one end partially positive or negative than the other end. Different charge will
Premium Atom Electric charge Molecule
The objectives of this experiment were to investigate the activity of enzymes‚ components that influence the enzyme’s activity‚ identify an unknown phosphatase‚ influence of inhibitors‚ and determine if inhibition is competitive or noncompetitive. A spectrophotometer evaluated the measurement of color change over a period time due to product being formed. Determining unknown phosphatase and effects from different inhibitors were determined by varying the pH and substrate concentrations. The unknown
Premium
Junnel Metrillo Date Performed: October 27‚ 2014 2L Group 1 Date Submitted: November 3‚ 2014 Exercise 6 Dipeptide Sequence Determination To understand the chemical‚ structural and functional properties of a certain protein‚ it is important to determine its amino acid components and sequence. The differences in the composition and sequence of amino acids dictate
Premium Protein Amino acid Oxygen
CHEM 1474-E01 Chemistry for Everyday WEEK5 Lab Report Spring 2017 Instructor: Kyoung Nan Kim Office: SI3076 Email: kyoung.kim@ucdenver.edu Office hour: M/W 1:00p-2:30p (SI3076) T/Th 12:30p-1:30p (SI3076) • All communication should be done via school email (xxxx@ucdenver.edu) • The work will be submitted through canvas assignments. You can simply upload doc/pdf files under “Lab report” assignment tap. • Review: Ch2‚ Ch3.5‚ Ch 4.2‚ Ch6.1 and Ch7.1-Ch 7.3 in your text book (21st
Premium Atom Chemistry
amount of a chemically modified base is added. The four different batches are for the four different bases; a modified T goes in one batch‚ modified A in another‚ modified G in another‚ and modified C in the last. The modified bases cause the DNA sections to stop growing; The growing stops on the modified base that is inserted. The modified T will only have pieces that end with T‚ and so on. This causes the sections in the different batches to be different lengths. A florescent dye is also added into
Premium DNA Molecular biology Gene