● Cobalt(II) Chloride Hexahydrate CoCl2.6H2O‚ was weighed in a weighing boat. The Cobalt(II) Chloride Hexahydrate was transferred into a 100mL volumetric flask dissolved in water. ● 25 mL of the dissolved was measured‚ using a graduated cylinder and few of the dissolved Cobalt (II) Chloride Hexahydrate‚ was poured into test tube. The test tube was labeled with a tape‚ to signify 25 mL and the spectrophotometer was used to record the U-V visible ABS (absorbance spectrum) with the wavelength of 515
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Experiment 23: Synthesis of an Alkene April 5‚ 2013 Purpose: Results and Discussion The weight of our n-Butyl Bromide sample (actual yield) was 0.09g. The percent yield was found to be 4.38%. Only 0.09g of the theoretical 2.055g yield was obtained. The boiling point was 104° C‚ which is higher than the literature value of 101° C. This is due to the sample being somewhat impure‚ as impurities tend to increase boiling point. When IR spectroscopy was performed‚ there were no OH stretches
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6-hexanediamine and sebacoyl chloride with the loss of HCl via nucleophilic substitution. Cellulose triacetate was generated by mixing cotton balls and acetic anhydride with acidic condition under heating. Materials: Styrene‚ 1 M NaOH solution‚ CaCl2(s)‚ xylene‚ t-butyl peroxybenzoate‚ methanol‚ 5% aqueous solution of 1‚ 6-hexanediamine‚ NaOH(s)‚ 5% sebacoyl chloride in hexane‚ glacial acetic acid‚ concentrated H2SO4‚ cotton balls‚ acetic anhydride‚ CH2Cl2 Procedure: For the synthesis of polystyrene‚ 10
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Exp’t 84 Synthesis of Methyl Benzoate by Fisher Esterification from K. L. Williamson‚ Macroscale and Microscale Organic Experiments‚ 2nd Ed. 1994‚ Houghton Mifflin‚ Boston p385 Revised 10/15/03 Prelab Exercise: Give the detailed mechanism for the acid-catalyzed hydrolysis of methyl benzoate. Introduction: The ester group is an important functional group that can be synthesized in a number of different ways. The low-molecular-weight esters have very pleasant odors and indeed are the major components
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In this experiment‚ we used simple distillation and extraction to do synthesis of 1-bromobutane. The experiment was carried out by mixing 13.3 g of sodium bromide‚ 15 mL of water and 10 mL of n-butyl alcohol in a 100 mL round bottom flask. We cooled the mixture and added 11.5 mL of concentrated sulfuric acid. Then the mixture was heated at reflux with a short condenser for 45 minutes and then we drained out the condenser. We removed the condenser and distillation head was set up to set up the condenser
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infrared spectrum for the pure aspirin from the synthesis was taken by using crushed crystals‚ Figure 1. This spectrum was shown with different absorption bands corresponded with the functional groups of aspirin which was observed at frequencies > 1500 cm-1. These infrared frequencies were shown in Table 2. An infrared spectrum of an authentic aspirin was used to distinguish how authentic the infrared spectrum from the synthesis was shown in Figure 2 In this lab‚ aspirin was synthesized from salicylic
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The Grignard Synthesis of Triphenylmethanol Abstract: Grignard synthesis of triphenylmethanol was achieved by use of the Grignard reagent phenyl magesium brominde. The organometallic grignard reagent was synthesized by use of a reflux apparatus recrystallization techniques. Once synthesized it was used in a Grignard reaction that involved nucleophilic addition to a carbonyl in order to make triphenylmethanol. The final product was solidified and recrystallized and spectral data was
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enzyme glycogen phosphorylase b which is important to metabolism. AMP is an allosteric activator of the enzyme because it converts glycogen phosphorylase b from its T state to the R state which is the active form. Caffeine is an inhibitor because it binds the nucleoside inhibitor site. When it binds this site‚ it stabilizes the inactive T state and blocks the catalytic site which needs to be open for enzyme activity to occur. The glycogen phosphorylase b was purified with hydrophobic column chromatography
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Experiment 11.1 Conversion of t-Amyl Alcohol to t-Amyl Chloride Using HCL October 27‚ 2011 Purpose: Alkyl halides can be prepared from alcohols by reacting them with a hydrogen halide‚ HX (X = Cl‚ Br‚ or I). The mechanisms of acid-catalyzed substitution of alcohols are termed SN1 and SN2. The “S” stands for substitution‚ the “N” stands for nucleophilic‚ and the “1” or “2” stand for unimolecular or bimolecular. Secondary alcohols react with hydrogen halides by both SN1 and SN2
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Laboratory Exercise #3 Measuring Protein in Solution Abstract The purpose of this lab was to learn about the Biuret assay reaction to determine if it can detect proteins and amino acids; also‚ to understand the process of “salting out” proteins and how to determine the amount of protein in a solution. In order to do so‚ egg white and ammonium sulfate were mixed on ice and then put into the centrifuge. After PBS was added‚ the amount of protein could then be determined. After that‚ 14 test tubes
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