Introduction A sample of DNA found in a crime scene was provided along with five suspects. Their DNA was then processed using restriction enzymes and Agarose Gel Electrophoresis. The objective of this lab was to match a criminals DNA to a crime scene using restriction enzymes EcoRI and Pstl with Agarose gel electrophoresis. Restriction enzymes cut DNA at a specific base pair site recognized by the enzyme‚ which then turns one single strand of DNA into many fragmented strands of DNA. EcoRI recognizes
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DNA DNA‚ or Deoxyribonucleic Acid‚ is described‚ in Encarta Encyclopedia as a genetic material of all cellular organisms and most viruses. DNA carries the information needed to direct protein synthesis and replication. Protein synthesis is the production of the proteins needed by the cell or virus for its activities and development. Replication is the process by which DNA copies itself for each descendant cell or virus‚ passing on the information needed for protein synthesis. In most cellular
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Gel electrophoresis is a procedure which sorts molecules based on size and charge. The gel in gel electrophoresis refers to the object that separates the molecules. Electrophoreses refers to the force that is used to move the molecules through the gel. There are 2 stages to gel electrophoresis‚ separation and visualization. During separation the gel matrix is placed in an electrophoresis machine. An electric current is run through the machine and the different sized molecules form bands on the gel
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2015 DNA Lab BACKGROUND In this laboratory experiment‚ students were introduced to DNA electrophoresis. DNA electrophoresis is an instrument that many forensic scientists use to get a DNA fingerprint as an evidence for crimes. Not only can it be used for forensic science‚ people can use this for paternity test‚ as well as look for evolutionary relationships among organisms. Agarose is used to make the gel that the DNA fragments are going into. Since DNA particles are negatively charged‚ the gel is
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DNA and Crime Deoxyribonucleic Acid - the fingerprint of life also know as DNA was first mapped out in the early 1950’s by British biophysicist‚ Francis Harry Compton Crick and American biochemist James Dewey Watson. They determined the three-dimensional structure of DNA‚ the substance that passes on the genetic characteristics from one generation to the next. DNA is found in the chromosomes in the nucleus of a cell. "Every family line has it’s own unique pattern of restriction-enzyme DNA
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Title Application of DNA Barcodes to Identify Various Plant Species Abstract In this experiment we applied barcodes to plants in order to identify what species they are classified under. We also compared the DNA sequences of different plant species using the ribulose-biphosphate carboxylase gene (rbcL). We took samples from a plant called Chard and performed PCR‚ DNA amplification and quantification and sequenced the DNA. During the experiment‚ we hypothesized that this year’s “nonspinach”
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Experiment 2 Titles Extraction of Bacterial Plasmid DNA and Analysis of Extracted DNA Samples Objectives * To learn the procedures needed in extracting the bacterial plasmid DNA * To determine the concentration of original DNA sample and purity of prepared DNA sample by using spectrophotometer * To analyze the extracted DNA sample by gel electrophoresis Materials and methods (Refer to UDBB2144 Laboratory 2A Manual Principles of biotechnology page 6-10) Results
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bath as their ultimate pampering treat. How many fast-moving consumer goods segments can claim to satisfy such lofty needs with such a low ticket price? The beauty industry often cites the resilience of the colour cosmetics category in times of crisis – otherwise known as the lipstick index. It is time for the beleaguered bath additives segment to do the same and position their products as a luxury indulgence at prices accessible to most. Q: How is the economic environment impacting sales
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Samantha Meyer Lesson Plan 8/Reflection #8 SCIE 376 This week‚ I taught a lesson about fingerprinting to my students and it went extremely well. The students were highly attentive‚ every student participated‚ and based off of my assessment‚ every student fully understood the topic. My lesson this week aligned with the scientific and engineering practices 1‚ 2‚ and 4. Practice 1 is asking questions‚ practice 2 is developing and using models‚ and practice 4 is analyzing and interpreting data. My
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An example of modern forensics evidence is the use of DNA fingerprints. Sources of DNA include blood‚ hair‚ semen‚ saliva‚ bone and tissue. Each person has a unique DNA fingerprint. A DNA fingerprint is the same for every cell‚ tissue‚ and organ of a person. It cannot be altered by any known treatment. Consequently‚ DNA fingerprinting is rapidly becoming the primary method for identifying and distinguishing among individual human beings . An additional application of DNA fingerprint technology is the diagnosis of inherited disorders in adults
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