LABORATORY REPORT BIOLOGY CELL AND GENETICS (SB10203) TITLE: DNA Extraction from Banana DATE OF LABORATORY: 03/05/2013 LECTURER’S NAME: DR. LAM NYEE FAN DEMONSTRATORS’ NAME: MISS NOR EZANI AHMAD MISS LUSIA BAREK MOSES LABORATORY ASSISTANT NAME: MISS ROSILAH MOHD IDRUS STUDENT NAME AND MATRIC NUMBER: ELYAS ERIC HUIL(BS12110134) BONG SIN NENG(BS12110054) EDILAH NADRAH JOHANY( DIASSOFIA PAULA FRANKIE INTRODUCTION DNA is present in the
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DNA replication mechanism is an integral and the most important part of cell division. It is a complicated mechanism that requires a lot of steps and different enzymes. Many scientists were trying to understand the mechanism of DNA replication and its role in the transfer of the hereditary information to the offsprings. DNA replication begins with a single origin of replication that starts off the process by creating a replication bubble. In many eukaryotic cells‚ the DNA strand will have more than
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TRANSCRIPTION: Transcription is the process of which DNA matches corresponding RNA bases‚ Transcription is located in the Nucleus‚ and the only type of RNA that is involved in Transcription is mRNA‚ and the purpose is so that the code can get out of the Nucleus‚ mRNA is also made through Transcription‚ It also takes information that doesn’t directly make proteins but it helps makes codes for the production of proteins‚ DNA Transcription consist of 4 nucleotide bases‚ Adenine‚ Thymine‚ Cytosine‚ Guanine
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PLASMID DNA ISOLATION‚ RESTRICTION ENZUME DISGESTION AND AGAROSE GEL ELECTRIPHORESIS Abstract: The gel is covered with an ion- containing buffer‚ such as (TAE)‚ that controls the pH of the system and conducts electricity. overall DNA concentration was lower than expected. Using agarose gel electrophoresis is to separate and visualize the DNA fragment‚ which is produced by restriction enzymes . Introduction: The purpose of this experiment is to measure the size of the fragments of DNA and separate
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Quantifying the COX1 Gene within the Mitochondrial DNA of a Potato Introduction Respiration is a very important process for every living organism. While it is typically thought of as breathing in oxygen‚ and exhaling carbon dioxide‚ like all things‚ it must take place at the cellular level. The electron transport chain is responsible for cellular respiration. The process uses four complexes; the fourth is cytochrome c oxidase. Cytochrome C oxidase is responsible for the reduction of oxygen to water
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In order to read DNA‚ it must be sequenced. This sequencing uses electrophoresis‚ a technique that separates sections of DNA that differ by a base. Electrophoresis used to be done manually‚ but was error prone and time consuming. Now‚ automatic sequencing machines are used. A technician begins the process by pouring gel between two glass plates that are set less than half a millimeter apart. After the gel is set up‚ DNA is put into each of the ninety-six lanes. The DNA sections then move through
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We wanted to extract‚ see and analyze DNA from a single strawberry( 12.11 g). The long‚ thick fibers of DNA store the information for the functioning of the chemistry of life. DNA is present in every cell of plants and animals. The DNA found in strawberry cells can be extracted using common‚ everyday materials. To start the procedure we needed the following supplies; one strawberry‚ one plastic cup‚ 10 ml of Cell Lysis Buffer (10% detergent‚ 1% NaCl)‚ one plastic zip-lock bag‚ one coffee filter‚
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Report #2: Strawberries Pathway: The strawberry belongs to the family Rosaceae‚ genus Fragaria. They are one of the most widely consumed fruit throughout the world. This fruit can grow pretty much anywhere except places with extreme climate change. The United States is the largest producer of strawberries in the world. The U.S. strawberry growers mostly use fields to grow this perennial crop whereas other places use mostly greenhouses due to shorter growing seasons. The strawberries that we
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Module 2 Section 2 EXPERIMENT: DNA & Protein Synthesis Exercise 1 – Modeling DNA 1. List the four bases which are found in DNA. (1 pt) The four bases found in DNA are cytosine‚ adenine‚ guanine and thymine. 2. Fit any six nucleotides together to form a row‚ then list the six nucleotides in the order you used them. Work with your model pieces and try fitting the bases together to make a double strand as shown in Figure 9 of the lab manual. Which nucleotides form
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Bio 1 Lab: Electrophoresis and DNA fingerprinting Jani Lynette Hagen October 31‚2014 U74644799 Electrophoresis is a technique which uses an electric field to separate molecules‚ allowing for identification and characterization of the molecules. It is commonly used to separate nucleic acids and protein molecules of various sizes. To prepare the gel for electrophoresis the amount of agrose needed must be calculated. For a 0.8 percent gel 0.8 grams of agrose is necessary per 100 ml of buffer. The DNA
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