The pGLO lab is a lab where students attempt to put the genes that make a jelly fish glow into E. Coli. After a process called transformation‚ the process in which a cell takes up and expresses a new piece of genetic information‚ the E. Coli will be able to glow and will be antibiotic resistant. The students first need to learn a couple of techniques before they are able to begin this lab. The first technique they will need is how to keep their environment sterile. They must learn to only open their
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Introduction: To achieve genetic experiments with haploid organisms‚ genetic strains of different genotypes must be crossed from one another. Following fertilization and meiosis‚ the meiotic products can be analyzed as the ascomycete fungus‚ Sordaria fimicola. Sordaria can be used as a model to study meiotic segregation. The trait followed was the ascospore color. Ascospore color is a single gene trait therefore it is easily observed under a light microspore. Which allele is dominant is very tough
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Introduction In this lab‚ the purpose was to verify Hess’s Law. Four main topics were covered during this experiment including enthalpy of reaction‚ heat of formation‚ Hess’s Law‚ and calorimetry. The enthalpy of reaction‚ ΔHrxn is the heat or enthalpy change for a chemical reaction. The energy change is equal to the amount of heat transferred at a constant pressure in the reaction. The change represents the difference in enthalpy of the products and the reactants and is independent of the steps
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can survive in extreme environments. Halobacteria are also useful by being a good organism to perform DNA transcription‚ translation‚ and transformation on (Kramer‚ 2006). There are two different types of Halobacteria that are being observed in this lab. The first is NRC-1‚ which is also called the wild type strain. Although the pigmentation of the Halobacteria is caused by the production of the membrane protein‚ bacteriorhodopsin‚ which is a red‚ the wild type strain is pink in color. This pink color
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1. Introduction Microscopy is an essential technique not only in cell biology but natural science as a whole. We compared different types of microscopic techniques ‚ according to the specimen used and the scope of the experiment. Two specimens‚ stained and unstained‚ containing CHO cells‚ were prepared‚ examined and analyzed under the microscope using bright field (HF)‚ dark field (DF) and phase contrast (PH) settings. In addition‚ the four phases of cell division cycle were estimated.. Bright
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Abby Goldschmidt Honors Biology 2° Mrs. Gempel September 3‚ 2015 Daphnia Lab Results Paper Abstract The goal of the study was to observe the effects of multiple chemicals on a Daphnia magna’s heart-rate compared to a control (pond water). The different chemicals were caffeine and alcohol. The heart-rate was the main variable in this experiment. The Daphnia’s heart-rate was observed for 15 seconds and then multiplied by 4 to show its heart-rate in one minute. This was repeated 4 times for each
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Introduction Insert text for Introduction here … Methods Tissue preparation: Tracheal segments were isolated from male Wistar rats aged 6‐8 weeks from the Animal Resources Centre‚ Murdoch‚ and W.A. Tissues suspended in baths containing Krebs solution maintained at 37oC and bubbled with 5% CO2 in O2 throughout the experiments. Preparations were then equilibrated for 45 min to 500 mg of tension with Kerbs solution replaced after 15 min. The viability of each tissue was evaluated by examining
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A. Avril Crayfish Lab Report November 9‚ 2012 Dr. Marvin Results: Figure 1. Firing Rate of Tonic Receptor in Response to Stretch. The correlation between Firing Rate and Stretch of the slow adapting crayfish receptor for four different sets of data is represented in this figure. The recordings are taken at stretches of 2‚ 4‚ 6‚ 8‚ and 10 mm of the crayfish tail. The best fit lines for the different sets of data are as follows: Ali and Emily- Linear best fit line‚ Dave and Laura- Exponential
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Osmosis is a passive movement of water through a selectively permeable membrane moves from an area of higher concentration to an area of lower concentration. In this lab‚ we can determine the direction of diffusion of small molecules by measuring the diffusion of small molecules through dialysis tubing. This tubing acts as a selectively permeable membrane‚ and allow to pass larger molecules slowly. Introduction: Cells have kinetic energy and it causes the molecules of the cell to move around
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Melissa Kenney Dr. Braun/Lab K Lewis Symbols & Structures Purpose The purpose of this lab experiment is to practice writing Lewis symbols for elements and monatomic ions while writing Lewis structure for molecules and polyatomic ions. I will also be writing chemical equations using Lewis structures for
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