saliva‚ and bacteria can hydrolyze polysaccharides and proteins. Through this experiment we have found that heat and acid hydrolyze starch while using the Benedicts test. Using the same test in a different manner showed how saliva has a big effect on the breakdown of polysaccharides. Using the IKI test we noticed bacteria like B.Cereus are able to digest starch polysaccharides‚ where as E.Coli does not‚ and also the effect of bacterial protein on digestion. Bacterial proteins such as S. Marcescens
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ups Identifying food groups in unknown solutions Aim The aim of this experiment is to identify different food groups within several different unknown solutions. This will be carried out by placing the unknown solutions into separate test tubes and using different chemical indicators to see if any reactions occur. Each food group will act different when the chemical indicator is added‚ some will change colour while others will separate. Introduction Food is a vital source of energy that is
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Modified Winkler Method for Dissolved Oxygen Determination Tiffany T. Lee Department of Biological Sciences‚ College of Science University of Santo Tomas‚ Manila‚ Philippines 1008 Date of Submission: February 17‚ 2011 Abstract In this experiment‚ the Winkler Method was used to measure the dissolved oxygen (d.o.) in a water sample from the pond in the Arch of the Centuries which had the owl statues. Then‚ the amount of oxygen is determined through a series of reaction. Usually‚ Winkler
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10.0 EXPERIMENT ON DETERMINATION OF DISSOLVED OXYGEN Sl. No. Contents Preamble 10.1 Aim 10.2 Introduction 10.2.1 Environmental Significance 10.3 Principle 10.4 Materials Required 10.4.1 Apparatus Required 10.4.2 Chemicals Required 10.5 Sample Handling and Preservation 10.5.1 10.6 Precautions Procedure 10.6.1 Preparation of Reagents 10.6.2 Testing of Water Sample 10.7 Calculation 10.7.1 Table 10.7.2 Data Sheet 10.8 Interpretation of Results 10.9 Inference 10.10 Evaluation
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Testing To identify An Unknown The hypothesis tested was that depending on the solution presented‚ which would test positive for one of the following‚ proteins‚ carbohydrates‚ or lipids through use of chemical testing. (Sudan IV‚ Benedicts’ Solution‚ Iodine‚ Biuret’s) . In order to gain more information for the hypothesis‚ one must know how to test for said macromolecule. Each of the above stated molecules has their own individual solution that will in turn identify which molecule is present. The corresponding
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with the solution of Na2S2O3 the contents of each of the flasks T3 to T7 were titrated and a starch was used as an indicator. All the obtained results were recorded in a table. A graph of the titres of sodium thiosulphate versus time from the start of the reaction was plotted from which the order of reaction with respect to iodine was deduced. Method 2. Determining the rate of a reaction with respect to iodine Using the burettes the four mixtures of hydrochloric acid (HCl)‚ propanone (CH3COCH3) solution
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experiment is to see the enzyme amylase catalyse starch in a chemical reaction. | | Introduction Enzymes are proteins. They act as catalysts‚ allowing chemical reactions to take place by lowering the amount of energy needed. They therefore speed up a reaction without being consumed themselves in the process. Enzymes are needed by all human beings‚ without them we couldn’t survive. They act as catalysts that break down substances such as starch and convert them into different products such
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original colour of iodine‚ and what colour does it turn in presence of starch and glycogen? Starch: yellow --> blue-black (amylase in starch reacts with iodine) Glycogen: yellow --> reddish-brown (due to the multi-branched component) 5) How do plants and animals store simple sugar glucose? In the form of polysaccharides. In plants‚ starch is the polysaccharide with glucose units linked by glycosidic bonds. Glycogen‚ in animals‚ is a larger polymer. 6) How do starch and glycogen differ
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with Benedict’s solution. Name the nutrient constituent of food indicated by the test. Ans3. Sugar. Q4. A food-stuff shows a positive test with weak iodine solution. Name the nutrient content in the food-stuff. Ans4. Starch. Q5. Which sugar is obtained from sugar cane? Ans5. Sucrose. Q6. Which carbohydrate is present in maize? Ans6. Starch. Two mark questions with answers Q1. Why is mother’s milk best for the baby? Ans1. Mother’s milk is a balanced diet containing lactose (milk sugar)‚
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GROUP No.: | DATE: 02/14/13 | | NAME: | | EXPERIMENT No.25BACILLUS: Diagnostic Tests | | ASCOLI’S THERMOPRECIPITIN TEST Purpose: used to identify anthrax bacilli in animal hides and meat. Principle: This test was designed to detect B. anthracis antigens in the tissues of animals being utilized in animal by-products and thereby to reveal when these products contained ingredients originating from animals that had died of anthrax. The thermostable antigens involved are common to other
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