larger molecules such as proteins and polysaccharides(starch) that have dimensions significantly greater than the pore diameter of the dialysis tubing can pass through the tubings and they are retained inside the tubings.Smaller molecules such as water molecules and iodide ions are small enough to pass through the pores. The left-handed dialysis tubing contains 20%sucrose and iodine solution .The right-handed dialysis tubing contained 5% starch solution . The two entire dialysis tubings are submerged
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the colonies after addition of iodine on PDA plate‚ whether bubbles were observed or not in the catalase test‚ the color of the urea slant‚ the color of the citrate slant‚ and whether the rabbit plasma remained liquid or became solid due to clot formation‚ etc. After each observation note which results indicate production of the given enzymes and which results indicate non-production of the given enzyme by placing a “+” or “-“in the appropriate squares. A. Starch Hydrolysis Note: the color of
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pills. Should you? In the United States today many people have supposedly lost weight with certain pills. One of these pills is called carb cutter. Carb Cutter claims to inhibit the activity of the starch-digesting enzyme‚ amylase. When the diet pill binds to the amylase‚ it prevents the breakdown of starch. In the Biology 1103 lab‚ we conducted two experiments to determine whether or not the diet pill‚ Carb Cutter‚ actually worked. In each experiment we used an experiment tube and a control (blank)
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HKDSE CHEMISTRY – A Modern View (Chemistry) Experiment Workbook 5 Suggested answers Chapter 52 Importance of industrial processes Chapter 53 Rate equation Experiment 53.1 Determining the rate equation of a reaction using method of initial rate (A microscale experiment) 1 Chapter 54 Activation energy Experiment 54.1 Determining the activation energy of a chemical reaction 3 Chapter 55 Catalysis and industrial processes Experiment 55.1 Investigating the action of a catalyst 6 Experiment
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temperature on enzyme activity In this experiment… Independent variable: Temperature of the amylase Dependent variable: Enzyme activity which is measured by the time for disappearance of starch Controlled variables: Volume of amylase; volume of starch solution; concentration of amylase; concentration of starch solution Prediction of results i) At low temperature‚ the rate of amylase activity is very low. ii) At optimum (=best) temperature‚ the rate of amylase activity is the highest. iii)
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temperature. * Temperature influences time taken for iodine solution remains yellowish. No full marks will be given if students did not meet the 2 criteria of answer and the word ‘different’ / ‘influence’ is not specific. Students must try to avoid these words. Accurate response (3 marks): P1: Temperature of water bath P2: Time taken for iodine solution to remain yellowish (Horizontal observation) * At temperature 5˚C‚ time taken for iodine solution remains yellowish is 12 minutes. * At
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experiment is often known as the ’iodine clock’ experiment. Materials and apparatus: 1) 100cm3 beaker 2)burette 3)0.1M K2S208 4)0.1M KI 5)0.002M K2S208 6)starch 7)water 8)test tubes 9)stop watch Precautions: 1) The stop watch should be started after the potassium peroxysulphate solution just mixed with potassium iodide. 2) Drawes a cross on a white paper that will be easier to observe the intensity of dark blue intensity. Procedures: 1. KI‚starch and sodium thiosulphate were mixed
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VITAMIN C Section: Pre-Laboratory Answer the following questions before coming to the laboratory. Give the reason for the following; Iodimetric titrations are usually performed in neutral or mildly alkaline (pH 8) to weakly acid solution. 2. Iodine solutions are prepared by dissolving I2 in a concentrated solution of potassium iodide. 3. Iodimetric determination of vitamin C is performed rapidly after preparing vitamin C solution 9-1 E XPERIMENT 9 IODIMETRIC TITRATION OF VITAMINE
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bacteria. The starch hydrolysis test is used to determine the ability to hydrolyze starch. Bacteria use amylase‚ an extracellular enzyme to hydrolyze bonds linking the glucose subunits. Starch is detected by adding iodine to the medium. If the bacteria growing on the starch agar produce amylase‚ no color change will be seen around the bacterial growth because all of the starch is consumed. The absence of a color change is a positive test. Materials needed to perform this test include a starch agar plate
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cells. In experiment 5.1 in order to observe diffusion we examine the Brownian movement by observing carmine on a wet mount slide‚ and by using dialysis tubing‚ we investigate the selective permeability to reducing sugar‚ glucose‚ starch‚ and iodine potassium iodine. In experiment 5.2 we use samples of ox blood and Elodea plant to observe the net flow of water from the surrounding solution into the cells. In experiment 5.3 in order to estimate the osmolarity of the potato tuber cells we use the
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