Abstract This experiment was carried out to monitor the ability of the Barley Amylase Enzyme to effectively break down starch in solutions that are increasing in neutral pH. To do this the experiment was carried out so that tubes containing a reaction solution of the Amylase enzyme and starch were simultaneously mixed. The reactions were then introduced to I2-KI‚ which stopped the reactions‚ at two minute intervals. Each of these trials was repeated three times to ensure proper accuracy. After
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Laboratory ReportTitle: Observations of Chemical ChangesDate: 9/9/13Name: Purpose: The purpose of this experiment is for the student to gain knowledge and experience regarding how avariety of chemicals react to one another. In addition‚ the student should also gain a betterunderstanding of the household chemicals that they use in their day to day lives.Procedure:Observe the chemical changes that occur by mixing a list of chemicals (provided in the lab manual).Add 2 drops of the first chemical
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To determine if the reaction had gone to completion‚ a Starch/Iodine test was conducted. A change in the strip to a deep blue color would signal an excess of sodium hypochlorite reagent present. An excess is the reagent symbolizes the end of the reaction because there is no longer any reactant left to consume reagent. After the reaction was deemed completed‚ the product was isolated and purified by ether extraction‚ aqueous extraction and evaporation. To determine how sodium hypochlorite acts as
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how the concentration of Iodine changes with time and how varying concentrations of KI and H2O2 solutions affects the rate of reaction. 2 - Use an iodine clock: add 2cm3 of 0.1moldm-3 sodium thiosulphate (Na2S2O3) and 1cm3 of 1% starch solution at the start of the reaction. The added sodium thiosulphate reacts with the iodine as it’s formed: I2 + 2Na2S2O3 -> 2NaI + Na2S4O6 When the sodium thiosulphate is all used up the iodine remains in solution and reacts with the starch indicator to make the blue
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hyperthyroidism‚ menopause and lung disease can cause excessive sweating. The most common symptom people with hyperhidrosis is wetness. There are several test a doctor can perform to test for this. The starch-iodine test consist of an iodine solution being put on the area. After it dries‚ starch is then put on it and if there is excessive sweat‚ the area will turn blue. The paper test consist of a special paper being put on the area. The more the paper weighs‚ the more excessive sweat there is.
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Dialysis section is divided into two parts: test for copper(II) ion and test for starch. For Test for Copper(II) ion part‚ take 1 mL of the water from the dialysis beaker and place it in a test tube‚ and then add a few drops of concentrated ammonium hydroxide and record the observations. Lastly‚ for Test for Starch section‚ take 1 mL of the water from the dialysis beaker and place it in a test tube. Add a few drops of iodine solution and record
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OBJECTIVE: 1. To distinguish the bacteria abilities to metabolize various substrates and end products formed. 2. To observe the growth of different bacteria species in term of structures and its morphology based on different chemical substance applied. 3. To observe physiological and immunological properties utilized by different species of bacteria. INTRODUCTION: Bacteria biochemical testing can determine the types and numbers in terms of colony forming units of bacteria present in a
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have become a huge importance to industry and sparked a large interest into the exploration of enzyme activity in microorganisms. Amylase is one of the most widely used enzyme required for the preparation of fermented foods. Apart from food and starch industries‚ in which demand for them is increasing continuously‚ amylase is also used in various other industries such as paper and pulp‚ textiles‚ and medical labs. The global market for enzymes was about $2 billion in 2004 (Sivaramakrishnan et
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Carbohydrates There were many ranges of color changes in the Benedicts test and Iodine stain. For the Benedicts test‚ this oxidation/reduction response changes the arrangement of the reducing sugar creating a colored precipitate. The change in color is correlated to the first concentration of the reducing sugar. For the iodine test‚ the three dimensional structure of various polysaccharides permits them to react with the iodine stain to produce a certain color. As far as the molecular level‚ there is an
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after 48 hours. -If starch can pass through the semi-permeable membrane‚ then the solution outside the dialysis tubing will turn black. -If iodine (IKI) can pass through the semi-permeable membrane‚ then the solution in the cup will turn black. Activity B: Osmosis -If sucrose molarity influences osmosis then % change in mass will increase as molarity increases. Materials * Dialysis tubing * Plastic cup * Glucose/starch solution * Distilled water * Iodine-potassium iodide (IKI)
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