The catalase test is used to differentiate staphylococci (catalase-positive) from streptococci (catalase-negative). The enzyme‚ catalase‚ protects the bacteria from the toxic by-products of oxygen metabolism. This enzyme is produced by bacteria that respires using oxygen. The catalase-positive bacteria include strict aerobes. Catalase-negative bacteria may be anaerobes‚ or they may be facultative anaerobes do not respire using oxygen as a terminal electron acceptor. The test reaction is very fast
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A streak culture was performed on a Tryptic Soy Agar Plate for isolation and purity and then incubated at 37 degrees Celsius for 24 hours. A gram stain was performed to determine whether the bacterium was a gram negative or a gram positive. After performing the gram stain‚ I concluded that by the appearance of purple spherical clusters resembling grapes that is was a gram positive cocci. A Catalase test was then performed using hydrogen peroxide. A positive catalase test was observed
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Pseudomonas aeruginosa Pseudomonas aeruginosa is the bacteria of my initial unknown project. Determining which bacteria I had was completed by many steps. The one fact that I had‚ was that it was a gram negative bacteria because on the gram stain it appeared pink. Its cell wall is composed of a plasma membrane‚ periplasmic space‚ peptidoglycan and an outer membrane (lipopolysaccharide and protein). By looking at the agar plate it was easy to tell that it was not a swarmer and it did not have any
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Phelps‚ Biology 205 MW 2:10pm. Unknown Organism Paper The unknown organism tested in the Mesa lab was collected from in between the toes of my puppy‚ Riley. The original culture grown consisted of dozens of visibly different organisms of varying colony colors and growth patterns. I chose to test a smaller‚ red colony for my experiments. Based on its gram reaction and oxygen requirements‚ the following tests were performed to reach my presumptive ID: Test Performed Result Gram Stain +
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Gram Negative Unknown Lab Report Abstract The Unknown Gram Negative bacterium inoculated in a Tryptic Soy broth medium was randomly selected from a group of other unknowns. In order to identify this unknown the seven different types of biochemical tests will be conducted on this unknown bacterium to identify it out of 6 possible bacteria; Escherichia coli(E. coli)‚ Enterobacter aerogenes (E.aerogenes)‚ Klebsiella pneumoniae (K.pneumoniae)‚ Proteus mirabilis(P.miranilis)‚ Pseudomonas aeruginosa(P
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The first test conducted on unknown bacteria 32 was the Gram stain. From this stain‚ unknown 32 was found to be a Gram-positive cocci. This test eliminated all possible Gram-negative bacteria‚ Gram-positive rods and Gram-positive spirillium. Next‚ the endospore test determined whether or not the Gram-positive bacteria contained endospores. With the use of malachite green‚ steam‚ and safranin it was found that unknown bacteria 32 did not contain endospores. This eliminated Gram-positive cocci Sporosarcina
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As a result of the tests ran‚ I was able to identify the unknown bacteria. For Unknown A‚ I ran six tests. I first isolated the bacterium from the second bacterium and found a clear growth (Table 1‚ Figure 1). Secondly‚ I ran a gram stain and found a gram positive‚ cocci bacterium (Table 1‚ Figure 2). Third‚ I ran a catalase test in which was negative (Table 1). From here‚ I determined a starch hydrolysis test would be necessary to distinguish between different bacteria. The result was negative (Table
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The Role of Agarase in Agar-Degrading Bacteria Abstract Agar-Degrading (agarolytic) Bacteria is physiological class of bacteria capable of utilising agar as a sole carbon source. This ability is made available by the use of agarases - enzymes which break down agarose into oligosaccharides. This physiological class branches through genii‚ regardless of Gram Stain status or morphology. Through a review of scientific literature we can find identification methods‚ optimum conditions and the
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bacteria need to be separated and isolated before they can be identified. Moreover‚ gram staining was used to understand the basic morphology of these bacteria. As these plates were incubated and grown‚ the presence of two separate bacteria colonies was visible. The colonies from the mannitol salt were used to incubate a TSB broth to grow the gram-‐positive culture. The purity of this broth was tested using gram-‐staining technique. A circular colony from the TSA plate was used to incubate a TSB broth
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[either flexneri or sonnei‚ we used both in our lab during the semester]. Procedure {and observations}: Observe bacterial colony morphology. {Colonies are large‚ beige or cream-colored‚ with irregular borders.} Prepare two slides for gram staining and viewing under a microscope. {Either my gram-stain slides were bad or the microscopes I chose for viewing were not good. No bacteria were found under the microscope.} Prepare another gram stain of the unknown bacteria. {Gram-positive‚ coccus
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