lutea‚ Pseudomonas fragi‚ Micrococcus luteus‚ Alcaligenes faecalis‚ Clostridium sporogenes‚ and Micrococcus roseus. There were several qualitative tests that could be conducted to determine the identity of the unknown species‚ for example‚ Gram staining‚ Fermentation‚ Catalase‚ Oxidase‚ Starch Hydrolysis‚ Litmus Milk‚ MOI medium‚ and the Gelatin Test. All tests and techniques used were performed in accordance with The Microbiology Lab Manual. Materials and Method: A stock broth culture of
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Traditional Analysis of Unknown Colony Morphology Color- light yellow pigmentation Form- circular Elevation- convex Margin- entire [staphylococci] DNase Test This test is used to detect if the bacteria contains any deoxyribonuclease activity. Because no color change was observed from blue to clear my unknown bacteria displayed a negative result. Blood agar plate This test is used to detect the hemolytic activity in the bacteria. A darkish green color on the media around the
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UNKNOW BACTERIA LAB REPORT UNKNOWN 36 Introduction The purpose of this lab was to identify two unknown bacteria from a mixed culture. The reason for identification of unknown bacteria was to help students recognize different bacteria through different biochemical tests and characteristics. This is important in the medical field because identification of unknown bacteria can help treat a patient by knowing the contributing source of a disease. Also knowledge of different bacteria helped others
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Review for Microbiology Test #1 (Lesson 1-5) Lesson 1 What is the #1 killer of man worldwide? Heart Disease. What is the #3 killer in the US? Infectious Disease. What is the importance of MO in our world? List 6-8 reasons MO are important. Can’t live in Germ Free World‚ Keeps Economy running‚ Agriculture‚ Medication‚ Baking‚ Cosmetics‚ Paints‚ Fertilizers‚ Helps develop immune system‚ Decomposition of dead plants and animals to enrich the soil. What are the 4 groups of people most prone to
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Sarah Helms Thursday/ 11:15am #1 Escherichia coli There are many types of microorganisms and ways to treat each one. Knowing the differences of each is vital to treat a patient correctly. The purpose of this report is to explain the process and steps used to identify a certain microorganism referred to as the unknown. Materials and Methods A test tube with an unknown microorganism will be retrieved. Once the test tube is retrieved‚ a steak for isolation will be completed in order to produce
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to make a presumptive identification of the EI. The EI sample was taken from a door handle to the George Lynn Cross Hall‚ which is touched by hundreds of students entering the building every day. The most important exercises performed include gram staining‚ which
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The plate count agar‚ CFC agar and cetrimide agar are used and provided different evidences on the isolation of Pseudomonas from the soil experiment. Firstly‚ the plate count agar is a medium for the enumeration of viable organisms in food‚ water‚ waste water and also from clinical samples‚ and thus it is non-selective to any species. Whereas the CFC agar is a selective agar which contains reduced amounts of cetrimide but also cephaloridine and fucidin are added to allow the isolation of most Pseudomonas
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The aseptically inoculate nutrient broth tubes A and B did not have any microbes inoculated into the tubes so it should not have been cloudy and that was what was seen. Tube C should have been cloudy because it had E. coli inoculated into the nutrient broth and that was also seen. A simple streak was also performed and it looks has if the loop went over previous streaks which should not have been done. A complex streak was also done. No colonies grew towards the end of the streak‚ this could be due
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Discussion After doing a series of different biochemical tests for the isolated organisim from the sputum sample‚ it was concluded that the unknown Gram positive organism was Streptococcus pyogenes. The sputum sample was first inoculated in the agar plates MAC‚ SBAP‚ CHOC and also a direct gram stain of the sample was performed. After a day of incubation‚ the MAC agar gave a negative result‚ therefore it is not a Gram-negative bacteria and non-lactose fermenting. The SBAP and CHOC showed growth
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POLHN Microbiology Student Name: SOKOPETI FAKAOSIFOLAU. Assessment 6 – Gram negative bacilli 1. Which of the following is not true of Enterobacteriaceae? a. Gram negative bacilli b. Oxidase positive c. Reduce Nitrate to Nitrite d. Ferment glucose with acid production 2. Which of the following organisms does not belong to the Enterobacteriaceae group? a. Shigella flexneri b. Eschericia coli c. Proteus mirabilis d. Pseudomonas aeruginosa 3. Name the four species of Shigella
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