multiple types of testing in order to determine the identity of the unknown organism. The tests performed to identify the unknown organism included Gram Staining‚ Fluid thioglycollate/aero-tolerance test‚ Methyl Red-Vogues Proskauer‚ SIM test‚ Lysine Decarboxylase test‚ Lactose Fermentation test‚ Simmons Citrate test‚ and TSI test. The Gram staining technique showed that the organism was Gram negative and bacilli shaped. The fluid thioglycollate test showed that the organism was a facultative anaerobe
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further tests had to be conducted to fully confirm. Bacteria A showed a white opaque growth in both of its plates. Secondly both bacteria had to be gram stained‚ so that they could be identified as gram-positive or as gram-negative. The same gram staining method was used for both bacteria‚ which consisted of inoculating each bacteria into a
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carried out in properly supervised manner to compare the unknown bacteria to the six known that were mentioned above. First it was the Gram staining procedure used to categorize bacteria in two groups‚ gram positive and gram negative. Gram staining is one of the most useful test used in the clinical setting to identify bacterial colonies due to their broad staining spectrum. (Black‚ 2008‚ pp. 70-71) The basis of the Gram stain is that gram positive bacteria retains the color of the primary dye‚ the
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Questions 1. Describe the major differences between gram positive and gram negative bacteria cell walls. The gram negative bacteria cell wall is a thin peptidoglycan layer and an outer cell membrane with a lipopolysaccharide layer. The gram positive bacteria cell wall is a single thick peptidoglycan layer. This wall forms in a mesh like formation of three layers of alternating material. 2. From the procedure that you have carried out‚do you feel that the Gram positive stain is a simple procedure
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Observation The authors of this experiment‚ The Effects of some Inhibitors on the Rates of Photosynthesis and Respiration by Chlorella‚ knew that the rates of photosynthesis and respiration of many plants are affected by an adding chemicals. (Yaun and Daniels‚ 2000) The authors in this experiment would like to know in which ways different inhibitors affect photosynthesis and respiration distinctly. Objectives How certain inhibitors affect photosynthesis How certain inhibitors affect respiration
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KARYOTYPING Karyotyping refers to use of a microscope to examine the size‚ shape‚ And number of Chromosomes in a sample of body cells. Extra‚ missing‚ Or abnormal positions of chromosome pieces can cause problems with a persons growth‚ development‚ and body functions. In karyotyping‚ the chromosomes in a cell are stained with a dye to make the bands of each chromosome visible. Banding helps show differences in structure among the chromosomes and helps arrange them into pairs. The chromosomes
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Macromolecules Lab Purpose: to determine a method of testing for macromolecules. Materials: Knowns: Unknown: Test Solutions: Glucose solution Unknown solution Iodine solution Gelatin solution Benedict’s solution Starch solution Biuret solution Oil Brown paper Water Procedure: 1. Create a data table. 2. Label 5 test tubes with known solutions. 3. Add 10-20 drops of each known solution to respective test tubes‚ do not mix pipets! 4. Add 3-5 drops of
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Introduction: The purpose of this lab is to use staining techniques and biochemical testing to identify an unknown bacteria using Bergey’s manual. Bergey’s manual of Systematic Bacteriology is a dichotomous key primarily used to identify a bacterial species. Biochemical tests are used to differentiate different species of bacteria. These tests are effective in determining the characteristics of the microbe being tested. Such characteristics include citrate utilization‚ gelatin hydrolysis‚ nitrate
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agar to check for _______. Name This Technique. What is its purpose? What type of hemolysis is pictured below? Identify the Following I go straight from the 4x objective to the 100 x objective? Why? Identify the Following Identify the staining procedure pictured. Identify what we are doing here. What type of plate would we be making using this technique? Name the Structure Pictured. What Settings Do We Use to Sterilize Bacteria Using this Instrument? What temperature do we use to sterilize
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distinguishes between Gram-positive and Gram-negative bacteria based on the composition of the cell walls. Gram-positive bacteria appear purple and Gramnegative bacteria appear pink after staining. The first Gram stain produced unsatisfactory results and was then repeated with a clear indication of negativity. Light pink staining was evident on the cells in the field of view (FOV) and a search of the slide revealed uniformity in the sample. From these results we
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