I) Introduction In this lab‚ a test was conducted to determine how the relative redox activity of chloroplasts from spinach leaves‚ which were performing photosynthesis‚ would change when in the presence or absence of light. To observe these changes in redox activity‚ the chloroplasts were exposed to DCPIP‚ a chemical that changes color according to such activity. By determining the redox activity of the chloroplasts‚ it could then be inferred which chloroplasts were photosynthesizing more actively
Premium Experiment Photosynthesis Null hypothesis
Lab Report #1 Photosynthesis Lab Report: The Effects of Varying Concentrations of CO2 on the rate of Photosynthesis in Spinach Leaves Introduction Photosynthesis is the process by which green plants and some other organisms use sunlight to synthesize foods from carbon dioxide and water. Photosynthesis in plants generally involves the green pigment chlorophyll and generates oxygen as a byproduct. The purpose of this experiment is to see how different concentrations of carbon
Premium Photosynthesis Oxygen Carbon dioxide
The Role of Phytochrome in Lettuce Seed Germination Abstract This experiment was performed to determine the role of phytochrome in lettuce seed germination. In order to conduct this experiment three different types of Grand Rapids lettuce seeds were chosen: Waldmann‚ Concept‚ and Two Star. Each of these seeds were exposed to the following regulated courses of light exposure for ten minutes after they had imbibed for five minutes: Red‚ White‚ Far Red‚ and Darkness. It was hypothesized that the
Premium Seed Plant morphology Germination
This experiment is concerned with identifying photosynthetic pigments found in spinach plants and determining the spectrum of light each absorbs. By using paper chromatography‚ different pigments of spinach leaves can be separated. The knowledge gained in this experiment is relevant to understanding how the process of photosynthesis works. A Real-world application for this includes the harvesting of clean energy sources‚ as scientific advances have led the way to artificial photosynthesis on the
Premium Photosynthesis Carbon dioxide Oxygen
Chromatography lab Purpose: To separate food colorings into their component dyes using paper chromatography. Materials: Chromatography paper‚ Food coloring‚ Ruler‚ Pencil‚ Solvent solution‚ Test tubes‚ Test tube rack. Safety precaution: wear aprons‚ to make sure that you don’t get any of the alcohol on your clothes‚ and if you break a test tube you don’t get glass on you. Procedure: See-attached handout. Results: See chromatography with Audrey’s lab report.
Premium Food coloring Color E number
Liquid Chromatography – Laboratory #18 Introduction: We are using liquid chromatography to separate the colored substances in grape-flavored drinks. We separate the component dyes‚ and then we separate the flavorings and citric acids. Background: Chromatography is a process that is used to separate a substance into its component parts. The separation occurs between the stationary and moving phase of the lab. The moving phase consists of a fluid and the stationary phase consists of a solid
Premium Chromatography Gas chromatography Analytical chemistry
Problem How can you tell pigment separation by using Chromatography? Objective To prepare a chromatogram‚ separate pigments in a leaf and interpret the chromatogram. Hypothesis If I am to put a chromatography paper into a solvent‚ then it would separate the pigments depending on their Rf value. I think the pigments will separate in this order: Chlorophyll A‚ Chlorophyll B‚ Carotene‚ and Xanthophyll. Introduction Chlorophyll is the molecule that absorbs sunlight and uses its energy
Premium Chromatography Analytical chemistry Silica gel
Title Page Chelsea Maksin Organic Chemistry Laboratory 1 Column and Thin Layer Chromatography: The Separation of Spinach Pigments Russ Hoburg 02/15/2012 Abstract The main goal in doing the column and thin layer chromatography was to separate spinach extract into its components based on polarity and then to analyze the components. The separation of the spinach extract was done using the column chromatography with the wet/slurry packing method. Alumina was inserted into the column to act as
Premium Psychology Family Management
BIOLOGY DCN PHOTOSYNTHESIS LAB # 6 Effect of varying coloured filters on the photosynthetic rate of spinach chloroplasts The data below is just representative of what trends and relationships you were supposed to see. Numbers can vary. Absorbance at 620 nm for each treatment DCPIP + chloroplasts t=0 min. 0.93 0.945 0.905 0.915 t=3 min. 0.95 0.731 0.83 0.816 change in A620 -0.02 0.214 0.075 0.099 t=0 min. t=3 min. change in A620 t=0 min. t=3 min. change
Premium Photosynthesis Light Chlorophyll
com Liquid Chromatography AP Chemistry Laboratory # 18 Publication No. 10535A Catalog No. AP9093 Introduction In this experiment‚ liquid chromatography is used to separate the substances that are present in grape-flavored Kool-Aid®. First‚ the dyes responsible for the purple color‚ FD&C Blue #1 and Red #40 are separated. Then‚ in a second experiment‚ the other components of Kool-Aid®‚ the flavorings and citric acid‚ are separated as well. Concepts • Resolution • Liquid chromatography • Selectivity
Premium Chemistry Distillation Food coloring