atoms and molecules are determined. This technique is spectroscopy where it absorbs‚ emits‚ or scatters electromagnetic radiation. Electromagnetic radiation has particle-like properties having photons and quantum and wave-like properties with frequency and wavelength. To make molecules visible through light‚ the wavelength of the molecule should be longer than the wavelength of the light. One spectroscopic
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Lab Report 4 Biology 103 Prof. Dr. Johnson Spectrophotometer; the finding of protein concentration of an unknown sample of BSA‚ and by using the standard curve. Abstract A spectrophotometer’s purpose is to use colors of the light spectrum to determine the concentration of light absorbing molecules in a solution. (p.59) In this particular lab‚ our mission was to determine the protein concentration and the standard curve of the unknown sample of BSA. This‚ by preparing five dilutions of
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CH 202 Breakdown UV/Vis: Molecules containing π-electrons or non-bonding electrons (n-electrons) can absorb the energy in the form of ultraviolet or visible light to excite these electrons to higher anti-bonding molecular orbitals. The more easily excited the electrons (i.e. lower energy gap between the HOMO and the LUMO)‚ the longer the wavelength of light it can absorb. Conjugation raises the energy of the bonding orbitals and lowers the energy of the antibonding molecular orbital. This
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Background NMR spectroscopy is a well established method for structure determination of various organic molecules ranging from small molecules all the way up to large bio-molecules such as proteins‚ DNA and polysaccharides. The chemical environment surrounding each atom as well their proximity to other atoms in a molecule can be studied with various experiments‚ enabling high-resolution 3D-structure determinations as well as verification of the chemical structure. NMR spectroscopy is well suited
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Module 2 Mass Spectroscopy [pic] [pic] Diagram of a mass spectrometer Stage 1: Ionisation The sample is injected as a vapour and the atoms are bombarded by fast moving electrons. This causes the molecule to fragment into two or more pieces. When a molecule or part thereof fragments‚ one portion would be positively charged while the second portion will be uncharged. All uncharged fragments are ultimately lost in the machine as mass spectrometers only work with positive ions. Stage 2: Acceleration
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Callam 1/8 CHEM – 254 – WI -2009 Problem Set #1 – IR Spectroscopy - KEY 1. Which of the indicated bonds absorbs at the higher frequency in an IR spectrum? a. O or OH C=O > C-O b. N or NH C=N > C-N c. H or H C-H sp2 > C-H sp3 Callam 2/8 CHEM – 254 – WI -2009 2. What major IR absorptions are present above 1500 cm-1 for each compound? a. b. O sp3 C-H stretch 2950 C=O - ketone 1715 cm-1 cm-1 c. O sp3 C-H stretch 2950 cm-1 sp3 C-H stretch 2950 cm-1
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0 PANJAB UNIVERSITY CHANDIGARH- 160 014 (INDIA) (Estted. under the Panjab University Act VII of 1947-enacted by the Govt. of India) FACULTY OF SCIENCE SYLLABI FOR M.Sc. (TWO YEAR COURSE ) IN CHEMISTRY 1st & 2nd YEAR( Semester System) EXAMINATIONS 2013-2014 --:O:-- 1 OUTLINES OF TESTS‚ SYLLABI AND COURSES OF READING FOR M.Sc. FIRST YEAR (SEMESTER-I) EXAMINATION OF 2013 OBJECTIVE OF THE COURSE To teach the fundamental concepts of Chemistry and their applications. The syllabus pertaining
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Name: Chen Yao Le Lab Group: Tues Email: A0111185@nus.edu.sg Matriculation Number: A0111185R Date of Experiment: 17 February 2015 Experiment 6: Synthesis‚ Electronic Spectra‚ Structural Isomerism: Octahedral Co(III) complexes with Chloride and Ethylenediamine Ligands Abstract: In this experiment‚ Δ/Λ-[Co(en)3]Cl3∙4H2O was synthesized and the absorbance in UV-vis spectrum was recorded
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CHEM7113 - Atomic Spectroscopy ! !! Analytical Techniques "! Absorption (AAS) "! Flame AAS "! Vapour generation AAS furnace (electrothermal) AAS "! Graphite "! Emission (AES) "! ICP-AES "! AFS (atomic fluorescence) Some diagrams and Tables are reproduced from Skoog ‘Principles of Instrumental Analysis’‚ 5th/6th ed. 1 ! Atomic Spectroscopy ! !! Electronic transitions : UV - visible radiation excitation of outer-shell electrons of atoms in the gas phase energy
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EXPERIMENT : Iron in Breakfast Cereal by Atomic Absorption Spectroscopy OBJECTIVES : 1) To determine the actual iron content of different brands of cereals 2) To compare the experimental results with the values listed on the manufacturer’s labels. INTRODUCTION : Iron is one of the important minerals that is required for our bodies to function properly. Most of the iron in our body is found in the blood such as haemoglobin‚ approximately 60 -70% of the human body’s iron is found
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