3.6.5. Southern hybridization Southern blot hybridization analysis was carried out using standard procedures (Sambrook et al. 2001). Approximately 20 µg of total genomic DNA from transgenic plants and untransformed control plant was digested with EcoRI‚ and then separated by electrophoresis on 0.8% agarose gel. Digestion profile was checked with 1µg DNA‚ with single cutter enzymes EcoRI (10U) in T-DNA region. For southern experiment‚ 20µg DNA was digested overnight. Restricted fragments were separated
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The southern economy flourished on the backbones of the men and women that were enslaved in the 18th and 19th centuries. With the threat of the removal of the core method of profit for hundreds of plantation owners‚ an anger rose that would lead to an illogical reaction of withdrawing their entire region from the country that had built them. In essence the South felt a strong need to protect what they believed they possessed: the use of humans as components in the profit machine. This fierce protectiveness
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A Good Man Is Hard To Find Flannery O’Connor was one of the most known authors for writing southern gothic short stories. Southern gothic became a style of writing in the wake of the civil war and brought up questions in society like‚ ‘Why is violence such a large part of the south’s culture?’ and‚ ‘How did the South have such a hard time picking itself up after its defeat in the war?’ Southern gothic is usually decayed‚ grotesque‚ or derelict settings and situations and had themes of ambivalent
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There are lot of countries that helped and donated relief goods to the people of Samar and Leyte‚ they supported the Philippines to build and create new homes and infrastructures to start up new beginnings in the lives of the survivors. Until January 2014‚ the pain and traumas was still in the heart and mind of the survivors‚ but then there is
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Southern Colonies The southern colonies consisted of five of the first thirteen colonies‚ which were North Carolina‚ South Carolina‚ Georgia‚ Maryland‚ and Virginia. Where the climate was the warmest out of the three colonial regions‚ which to those living there was a good thing because survival in the winter was easier for them. Even back in the day‚ money has been an issue for many. This is why many colonist from New England decided to move to the Southern Colonies. In the southern colonies‚
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Brianna Smith March 8‚ 2012 Honors English II Southern Gothic Literature Southern Gothic Literature is a genre that focuses on events occurring in the American South after the Civil War. It involves supernatural events‚ monsters‚ heroes‚ and must reveal social and cultural issues. Boo Radley‚ a character who lives in Maycomb‚ fits the supernatural role in Southern Gothic Literature. To fit this role‚ ironic and unusual events should occur involving that person. Boo does a lot of unusual things
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Tinsley Eng 151C-33 3/08/2013 Southern Gothic Fiction is a genre of literature unique to the American south. Major influences of the genre itself were the culture‚ religion‚ and economic standing of the south at the beginning of the 20th century. Many who read southern gothic are transported into a grotesque fantasy world where ideas of death‚ good versus bad‚ and god are all prevalent. Many American authors were greatly influenced by the ideas of southern gothic literature such as “Harper
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Franciscan College of the Immaculate Conception Baybay City‚ Leyte S.Y.: 2014-2015 Project In Ed.Tech. I (Compilation of lessons 1-6) Submitted by: Pamela P. Sanchez BSEd-II Submitted to: Mrs. Marilyn Roguel Lesson I. History of Educational Technology The Evolving Concept of Educational Technology Ancient Greece = formulated cognitive rules Middle Age = Pierre Abelard introduced the technology of instruction. =Comenius‚ Pestalozzi‚ Froebel‚ Herbart and Montesorri contributed their
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The excuses whites used during Reconstruction to torture and murder newly freed African Americans were as false as they were numerous. In Southern Horrors and Other Writings‚ Wells relates many of these. Excuses ranging from sassing whites to rape to murder prove that "colored men and women [were] lynched for almost any offense" (Wells 78). According to Wells‚ the three most common excuses used to victimize African Americans during and after Reconstruction were that the victim had participated in
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blue waters. The island of Biliran is named after a creeping plant called “Buro-Biliran.” This magnificent and extraordinary island is found in the Eastern Visayas region here in the Philippines. Biliran Island‚ historically‚ is a part of the Leyte Province but somehow‚ it is turned to a small but united province. Biliran is filled with prestigious landscapes and extraordinary landscapes. With its eight simple and cultural towns‚ Biliran is envied by many provinces here in the Philippines
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