C. partellus gut protease and protease inhibitor activity assays. C. partellus gut protease activity was estimated using chromogenic substrate BApNA [Manasi A et al.‚ 2005]. It was dissolved in the DMSO and a final concentration was made to 1mM in 1.0ml‚ Glycine-NaOH buffer (pH 9)‚ assays were carried at 38°C for 30min [Manasi A et al.‚ 2005]. The reaction was terminated by adding of acetic acid (30%) of 200µl [8‚ 9‚ Manasi A et al.‚ 2005 ]. Supernatant (0.5ml) was added to 1M NaOH (0.5ml) and the
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According to Stability testing of new drug substances and products‚ a guideline of ICH desires that to clarify the inherent stability characteristics of the active component stress testing was implemented. The aim of this work was to carry out the stress degradation studies on the Lamivudine‚ Abacavir and Dolutegravir using the proposed method. Formulation drug products were exposed to thermal stress‚ oxidative stress‚ photolytic‚ hydrolytic stress under acidic medium and basic medium. An ideal
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Materials and methods Chemicals Triton X-100‚ abscisic acid‚ sodium nitroprusside‚ methyl jasmonate (95%) and phenylalanine were purchased from Sigma Aldrich (Mumbai‚ India)‚ sodium hypochlorite and salicylic acid from Hi-media (Mumbai‚ India). The other chemicals were of analytical grade and obtained commercially. Plant material and callus induction Carrot seeds (var. Atomic red) were collected from Denmark (plantfro). Seeds were surface- sterilized with 75% ethanol for 1 min‚ and then soaked in
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The purpose of the lab “All in the Family” was to compare the reactivity of the halogens Chlorine‚ Bromine‚ and iodine by observing the reactions between their elemental forms and their ionic forms. To accomplish this experiment‚ we first added a squirt of pet ether to two test tubes. Then‚ we added the same amount of Cl2 to both of the tubes‚ shook the mixtures‚ and recorded the color that resided in each mixture as the initial color. After‚ a squirt of NaBr was added to one test tube‚ and a squirt
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Aspirin Aspirin is a salicylate‚ also known as a non steroidal anti-inflammatory drug (NSAID). It is the generic name of acetylsalicylic acid and is used clinically to treat fever‚ pain and inflammation as it works by preventing the formation of prostaglandin. Prostaglandin is the active agent that is responsible for the sensitisation at the nerve endings. It can be administered orally‚ rectally or by intravenous route. (6) Benzene ring Carboxylic acid Ester Alkane Aspirin has a pKa of 3.49
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The separation scheme was created by centrifuging a controlled sample of one of the ions or anions combined with one of the reagents such as NaCl. It was found that it could be separated out after centrifuging and would result in a sample without the ions or anions if it precipitated. With the data from week one a method of adding the reagents in a certain order would result in the ability to separate all ions or anions out of a solution. Overall‚ knowing how the cations or anions reacted with certain
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There are many chemicals in our world that are soluble‚ however‚ these chemicals have to follow a set of rules to determine how soluble each chemical is and if it is at all. This is what we call solubility laws. These laws show what chemicals are soluble and which ones are insoluble. They also describe what chemicals can be dissolved and what chemicals cannot dissolve. These chemicals are them recorded on Solubility tables which list solubility and how soluble the chemicals are. According to Busch
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Jackson Bellinger “Turning Waste Heat into Power” Physicists at the University of Arizona have discovered a way to convert waste heat back into useable electricity‚ using molecular thermoelectricity. In addition to making many machines much more efficient‚ molecular thermoelectricity would make CFC’s not needed. This new technology makes it possible to convert heat into energy with no moving parts‚ and no pollution whatsoever. Charles Stafford‚ the associate professor of physics at the University
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Once the aspirin is prepared‚ it is isolated from the reaction solution and then it is purified. The aspirin is insoluble in cold water‚ and it is isolated by filtering the chilled reaction solution. Purification is essential to remove any unreacted salicylic acid and acetic anhydride as well as the acetic acid product and phosphoric acid. Acetic anhydride is caused to decompose by the addition of water once the formation of aspirin is complete. C4H6O3 (Acetic anhydride) + H2O (Water) ------------------------->
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Methanolic Extract of Eggplant (Solanum melogena) Peel as a Dye Pigment in Differential Stains Azucena‚ Alexis G. An Official Entry to 2010 Regional Science Fair Cluster 2 Physical Science- Individual Negros Occidental National Science High School January 13‚ 2011 Ma. Melanie P. Romero Research IIB Adviser APPROVAL SHEET The research paper attached hereto‚ entitled‚ “Methanolic Extract of Eggplant (Solanum melogena) Peel as a Dye Pigment in Differential Stains”‚ prepared and submitted
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