Guidelines for writing laboratory reports Laboratory reports should be written according to the format below (failure to do so will result in marks being deducted): Formatting Font Type: Times Roman Font size: 12 Spacing: 1.5‚ justified Pages : 5 (minimum) - 10 (maximum) [pages must be numbered] Title page You are required to use the lab report submission page available on the LMS and are to include these details: lab no.‚ title of experiment‚ students’ names and ID‚ date of experiment as well
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they contain starch. The optimum pH for pancreatic amylase is the pH of 7. In the experiment I have used buffer solutions with the pHs of 2.8‚ 4 and 6.5. I have also used iodine and starch. Normally‚ iodine is orange-yellow‚ however when you add starch to it‚ the solution will turn blue-black. Aim: The aim for this experiment is to investigate how the different buffer solutions work on the enzyme amylase. This will be investigated by using iodine and by timing the experiment using the colorimeter
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Joanne Kathleen Saori T. Suzuki‚ Laurent Santos‚ Val Justin D.M. Tongco* College of Science‚ University of Santo Tomas‚ Manila Philippines Abstract Salivary amylase‚ found in humans‚ is enzyme that catalyzes the hydrolysis of starch into simpler compounds. Its enzymatic activity is affected by several factors‚ such as temperature and pH. The rates of enzymatic activity of salivary amylase in different temperatures and pH were measured and resulted to be very near 50 C and 7 respectively. However
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IODINE TEST FOR STARCH The Iodine test is used to test for the presence of starch. Iodine solution — iodine dissolved in an aqueous solution of potassium iodide — reacts with the starch producing a purple black color. The colour can be detected visually with concentrations of iodine as low as 0.00002M at 20°C. However the intensity of the colour decreases with increasing temperature and with the presence of water-miscible‚ organic solvents such as ethanol. Also the test cannot be done at very low
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Experiment 8: Hydrogen Phosphate Buffer Systems Purpose The purpose of the lab was to create a buffer solution and observe the capacity of the phosphate buffer system. Also‚ the experiment was meant to provide experience with the calculations and mathematics involved in creating the buffer solution. Finally‚ the lab was designed to provide an insight and appreciation for the necessity and complexity of buffers in our physiology and in the environment. Procedure No deviations were made from
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Experiment 1: "A cis to trans conversion" Saturday‚ June 25‚ 2005 4:19 PM Notes on Theory • Alkenes o They are hydrocarbons with a C=C double bond • Double bonds are stronger and more reactive than single bonds o Hydrocarbons with double bonds used to be known as "olefins"‚ because they had an oily appearance • "Oleum" means oil • "Ficare" means make • Isomers o Geometric isomers have the same molecular formula‚ but a different geometric arrangement • They also have different physical properties
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and heating the solution to boiling point. Upon reaching just below boiling point‚ decolorizing carbon was added to the solution‚ and the solution was allowed cooled for 2-3 minutes. The dark black solution was filtered through a gravity filtration system‚ leaving dark residue behind on the filter paper‚ and colorless to slightly pink liquid in the beaker. Then‚ 9 mL of sodium acetate buffer‚ to maintain a relatively constant pH and 1.8 mL of acetic anhydride were added to the solution‚ and then it
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in quite dilute aqueous solutions‚ acetic acid is very slightly ionized (it would approach 99% ionization only as the concentration approaches 0.0 M): HC2H3O2(aq) + H2O(l) Ka = 1.8 x 10-5. H3O+ + C2H3O2- In general‚ if the acid is not extremely weak‚ the pH of a solution of a weak acid is governed by the concentration of the acid and Ka. Under similar conditions‚ the pH of a solution of a weak base is determined by the concentration of the weak base and Kb. In solutions containing both a weak
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their pH levels in various titration solutions‚ using a pH meter. II. Procedure To start our experiment we had to prepare Buffer B‚ which was the .060 M Ammonia/Ammonium solution. Using 3.0 M ammonia‚ we had to calculate the proper volume of NH3 base by using the M1V1 equation. Calculation: Using pipettes we measured out the needed volume and transferred it to an Erlenmeyer flask‚ which we eventually used to combine the solution. We also had to calculate
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used: * Fehling’s Solutions A and B * Sodium hydroxide and copper (II) sulphate solutions 1. Add 2 cm3 of the liquid food sample* to a clean‚ dry test tube 2. Add 2 cm3 of Biuret Reagent. Alternatively: * Use sodium hydroxide solution and copper sulphate solution instead. Add 1 cm3 of sodium hydroxide solution (40% or bench solution) and 1% copper (II) sulphate solution dropwise – drop by drop - to the food sample * Use Fehling’s A and B solutions instead. Fehling’s A
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