Na+ H+ Wallert and Provost Lab Excel with hard work and inquiry Ammonium Sulfate PPT Protocol Theory and Introduction: Ammonium Sulfate Precipitation is a classic first step to fractionate proteins by causing perturbations in the solvent with respect to ionic strength. Historically‚ separation methods were limited and as a result precipitation methods were highly used with very fine cuts in ppt conditions. As more choices of inexpensive and quality resins are commercially available precipitation
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Experiment 5: Shifting Equilibrium A solution is in equilibrium when the rate of forward reaction and the rate of reverse reaction are equal. This equilibrium may be disturbed when the concentration of the reactants‚ the concentration of the products or the temperature is changed. If the process involves gases‚ a change in pressure can also affect the position of equilibrium. The concept behind this is Le Chatelier ’s Principle which states that when a system is disturbed through application
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Analysis of Alum AlK(SO4 )2 . 12H2O AP Chemistry Lab # 5 INTRODUCTION Every compound has a unique set of chemical and physical properties. To identify a compound with certainty‚ experiments have to be conducted in order to verify these properties. Such kind of analysis is usually performed when a compound is synthesized‚ and whether the compound is indeed the compound desired needs to be ascertained. The main purpose of the experiment is to analyze alum‚ AlK( SO4
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Title: Physical and Chemical Properties Purpose: The purpose of this experiment is to investigate the physical and chemical properties of pure chemical substances by subjecting them to various environmental extremes. Procedure: Filled each test tube with substances provided and subjected them to various conditions. These conditions included‚ heat‚ cold water‚ hot water‚ acid and basic additions and tested on litmus paper. The reactions were observed and documented at each step. Data tables:
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Question 1: You have carried out an investigation into the pigments that make up chlorophyll. Use the chromatogram you obtained when carrying out this investigation. Clearly explain the separation technique of paper chromatography in terms of partition. Calculate the Rf value of one of the constituent pigments and compare it to the book value. A paper chromatography was used to separate the pigments that make up chlorophyll to analyse‚ identify‚ and quantify the components‚ using paper (stationary
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Abstract: One of the primary considerations in preparing pharmaceutical ointments is the properties of different types of ointment base. The types of ointment bases used as vehicles for drugs are selected or designed for optimum delivery of the drugs and also to contribute emolliency or other quasi-medicinal qualities. Ointments properties vary since they are designed for specific uses‚ ease of application or extent of application. The purpose of the lab was to learn the difference between
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Purification of solids Aim: To separate a mixture of carboxylic acid and a neutral substance by treatment with aqueous sodium hydroxide and purifying the carboxylic acid‚ measuring the melting points of the neutral and the acid components. METHOD: A mixture of( 5g) Carboxylic acid and neutral compound were separated by adding 2 mol dm-3 sodium hydroxide (25cm3) to the mixture‚ which separated the water soluble sodium from the water insoluble neutral component‚ by filtration. The solid at the
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Filtration is commonly the mechanical or physical operation which is used for the separation of solids from fluids (liquids or gases) by interposing a medium through which only the fluid can pass. Oversize solids in the fluid are retained‚ but the separation is not complete; solids will be contaminated with some fluid and filtrate will contain fine particles (depending on the pore size and filter thickness). Filtration is also used to describe some biological processes‚ especially in water treatment and sewage
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The initial mass of copper wire was 1.250g but the recovered copper was only 0.120 subtracting the mass of the recovered copper with beaker from the tare beaker mass. So the percentage yield resulted only to 9.6% it is very little‚ and it may be due to many possible sources from doing the steps. Adding too much or too little of a compound to the copper solution‚or the loss of copper during transport or by being left on the stirring rod. Another situation which was a source of error is when the copper
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Fig. (3.12): Cylinders after being removed from the molds and curing Fig. (3.13): Cubes after being removed from the molds and curing Fig. (3.14): Prisms after being removed from the molds and curing 3.5.7. Preparation of specimen’s surface: The specimen’s surface is important since it has variable influences on mechanical properties. So the specimens were prepared to have a smooth and parallel bearing surface. These specimens were perpendicular to the axial load during all the tests‚ as
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