Part B: Gram Stain Preparation: Watch the following video: The Gram Stain (4:15 minutes) Procedure: Include the answers to the questions below in your lab report. Lab Questions: This procedure is used to separate what two types of bacteria? This separation is based on differences in what physical trait? What is the iodine used for in this staining technique? Why is it important to heat fix the bacteria prior to the staining procedure? If you had a bacteria sample that you knew to be gram
Premium Gram staining Staining
Introduction: The gram stain is one of several laboratory procedures that can be used to narrow down the identities of unknown bacteria. Bacteria have three different shapes; cocci‚ bacilli‚ and spirilla. Since bacteria pretty much have the same reflective index as water‚ a bacteria cell must be dyed so that these shapes can be seen. Materials: Petri dish Dropper Cleansing solution Slides Bibulous paper Inoculation loop Crystal violet dye‚ Iodine Acetone Safranin Water from the sink
Premium Staining Gram staining Microbiology
When I received my slant culture‚ I immediately made a streak plate and started the Gram Stain protocol1. In the first two slides‚ I made and stained‚ my results were ambiguous. Without putting the slide into the microscope‚ I noticed one was pink and the other purple. To resolve it‚ I discarded my slides and started again and was extremely careful in proceeding the Gram Stain. I concluded that my unknown A was gram-negative bacilli. Using the dichotomous key for gram-negative bacilli I started
Premium Bacteria Microbiology Escherichia coli
Exercise 3.9 Endospore Stain (Differential Stain) Eula Lewis Bio 2921 February 27‚ 2013 Zere Ezaz‚ Ph. D. Objective: 1. To learn to endospore stain 1. To distinguish endospores‚ free spore‚ and vegetative cells Theory: A dormant form of bacterium that allows it to survive poor environmental conditions are called endospores. Their tough outer covering is made of keratin and is also the reason they are resistant to heat and chemicals. The strength of keratin also makes it resist
Premium
MICROBIAL SURVEY‚ SMEAR PREPARATION‚ AND SIMPLE STAIN Instructional Objectives 1. Define Roccal = green‚ liquid disinfectant. Pathogen = an agent which causes disease. Wet Mount Slide = a microscope slide of a liquid specimen covered with a cover glass. Yeast = a single celled fungi. Budding = a true characteristic method of asexual reproduction among yeasts where budding of a new cell from a parent cell can be observed. Mold = multicellular masses of filamentous fungal growth. Hyphae
Premium Yeast Bacteria Fungus
Through processes know as wet mounts and simple stains‚ observing living microorganisms through a compound microscope can generate a clearer understanding of their appearances and movements. Microorganisms are living things that for the most part cannot be seen through the naked eye. They live on and among humans‚ as well as plants‚ animals‚ and all that is a part of the Earth. Harmless as most seem to be to humans‚ microorganisms are an important relevance of study for human interest in varied perplexities
Premium Staining Microscope Organism
interior of the cell from the exterior and regulate the movement of substances in and out of the cell. Diffusion is how substances more in and out of the cell. There are several types of diffusion‚ but for the purposes of this lab‚ we will be focused on simple and osmosis. Simple diffusion and osmosis are vital for the diffusion of water and maintaining homeostasis. Homeostasis is the ability to maintain equilibrium and keep the organism alive. For example‚ if a cell is placed in a hypotonic solution
Premium Cell membrane Osmosis Protein
Simple Sugars Lab Emerson: Hallie: Background: Benedicts solution is a chemical solution that changes color in the presence of glucose and other reducing sugars. It is a mixture of sodium or potassium citrate‚ sodium carbonate‚ and copper sulfate. It changes from its normal blue color to an orange‚ red‚ or yellow. it changes based on the amount of sugar used. "Benedicts Solution." Definition from BiologyOnline.org. N.p.‚ n.d. Web. 16 Jan. 2015. http://www.biologyonline.org/dictionary/Benedicts_solution
Premium
Microbiology 197 Prepared Bacteria Gram Stains (F12) Materials required: * Microscope; clean and properly set up * Immersion oil * Lens paper * Lens cleaning fluid * Microscope drawing forms * Specimens: 1. Bacillus subtilis 2. Staphylococcus aureus. 3. Escherichia coli Procedure: 1. Observe each of slides listed in “Specimens” above. 2. Make your observations using oil immersion (1000X). 3. Using a drawing form draw the organisms
Premium Staining Gram staining Bacteria
PCS125 Lab – The Simple Pendulum Objective and Background Objective: The Objective of this experiment is to examine the simple harmonic motion and to determine the value of the acceleration due to gravity from the analysis of the period of the simple pendulum. [1] Background: There are three equations that will be used to calculate the period of motion of the simple pendulum. They are the slope of the line of the graph of T² against L‚ and the gravity of the pendulum motion. The period of
Premium Pendulum Linear regression Regression analysis