Question 1 1.1 Silica gel chromatography: This is known as the stationary phase in column chromatography. Firstly‚ the tapered exit of the column is sealed using porous material. This porous material serves as support for the packing material‚ and prevents it from exiting the pipe. Thereafter‚ silica gel is compacted into the glass pipe to make the separating column. In finishing preparation of the column‚ the solvent which is used as the mobile phase is then passed through the dry column. Then the
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Introduction: Recrystallization is used for the purification of solid compounds. The recrystallization process relies on the fact that majority of compounds are more soluble in hot solvent than in cold. The hot saturated solution containing the compound will have unwanted impurities and will be filtered out and cooled to produce the pure crystal constituents of the compound. Thin layer chromatography can be used as a physical method to segregate compounds from natural sources. E.g. Spinach
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As we know‚ the TLC is a separation technique that has two phases: the stationary phase and mobile phase. The stationary phase is silica gel (SiO2) which is very polar compound‚ while the mobile phase is 0.5% acetic acid which has less polar. Also‚ as we know‚ the compound will rather to stay with stationary phase or travel with mobile phase depend on its polarity. The result show as that the polarity of the solvent is the key factor for the compound to travel with it or stay in stationary phase
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Making TLC Plates from Bulk TLC Silica Gels Many TLC users prefer to use pre-coated TLC plates‚ but others because of their special needs‚ or because they need special additives or a special thickness‚ coat their own TLC plates. This paper is a quick review of what is needed for the process. Initial instructions for plate coating: If reproducible plates are needed‚ then the user should invest in a coating device from Camag or Desaga (addresses below). The devices consist of a tray that holds
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was added to the product mixture in a 30mL beaker and about 300mg of silica gel was added 3. The beaker was covered with aluminum foil with a hole and was CH2Cl2 was evaporated. 4. 2.3g of fresh silica gel was mixed with 7mL of hexane in a 30mL beaker to get a slurry. 5. Stirred the slurry constantly with hexane added and poured it into the column. 6. Hexane was added and run until the solvent was about 1cm above the silica gel. 7. About 1cm of sand was added followed by loading of the dried
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Title: Thin-Layer Chromatography Objectives: Part1: 1. To learn the technique of TLC and the visualization of colourless components. 2. To identify an unknown drug by a TLC comparison with standard compounds. Part 2: To learn the separation technique by using Thin Layer Chromatography plate in separating a mixture of compounds into individual pure compound by using Spinach Leaf. Introduction: (i) General Concepts Chromatography is a common and powerful method used to separate and analyze complex
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best solvent system for separating a methylene blue/sodium fluorescein mixture by thin layer chromatography using silica gel and alumina as the stationary phase. In the second week‚ the students will separate a methylene blue/sodium fluorescein mixture by column chromatography with silica gel as the stationary phase. Part I: Thin Layer Chromatography (TLC) 1. Obtain 2 silica-gel-coated TLC sheets and 1 alumina coated TLC sheet. Measure up 1 cm from the bottom of the TLC strip. Taking care not
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Column Chromatography of Plant Pigments Jaybee Balilea‚ Sharmaine Baysic‚ Maria Anjelette Patricia Belen 3Bio-7‚ Department of Biological Sciences‚ University of Santo Tomas‚ Manila‚ Philippines Abstract Column Chromatography is a form of solid-liquid adsorption chromatography and depends on the essential principles as does in thin layer chromatography. It was used in this experiment in separating and analyzing the different components of Capsicum frutescens (siling labuyo) with the use
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of methanol and water (95:5)‚ using any suitable method as described in Test Solution under Thin Layer Chromatographic Identification Test for Crude Plant Drugs. The filter is the Test Solution. Application Volume—5 µL Plate—Chromatographic silica gel. Developing solvent system—a mixture of 1-butanol‚ 1-propanol‚acetic acid and water. (30:10:10:10) Spray reagent—ninhydrin TS. Procedure—Proceed as directed in thin layer Chromatographic Identification Test for Crude Plant Drugs. Develop the
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separate mixtures of naturally occurring compounds isolated from plants and other living organisms. Performing column chromatography involves packing a column‚ which is a glass cylinder‚ with the stationary phase. The stationary phase is typically silica “gel” or powdered alumina‚ depending on the types of compounds you wish to separate. The mixture is placed on top of the packing material and a steady‚ continuous flow of eluting solvent is passed through the column. Although in the TLC experiment only
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