procedure‚ an electric current is applied to the gel so that the separated proteins transfer through the gel onto the membrane. To detect the antigen blotted on the membrane‚ a primary antibody (serum) is added at an appropriate dilution and incubated with the membrane. Antibodies present in the serum bind to the protein(s). In order to detect the bound antibodies‚ anti-immunoglobulin antibodies coupled to an enzyme alkaline phosphatase or horseradish peroxidase are added. This anti-IgG enzyme is
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Dengue fever is a disease caused by one of a number of viruses that are carried by mosquitoes. These mosquitoes then transmit the virus to humans. The virus that causes dengue fever is called an arbovirus‚ which stands for arthropod-borne virus. Mosquitoes are a type of arthropod. In a number of regions‚ mosquitoes carry this virus and are responsible for passing it along to humans. These regions include the Middle East‚ the far East‚ Africa‚ and the Caribbean Islands. In these locations‚ the
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Cardiovascular System: Blood Purpose Explain why you did this lab and what if any safety precautions needed to be followed. The purpose of this exercise is to gain knowledge and become familiar with the components of blood and blood cells. In order to do this‚ a prepared slide as well as a slide using my own blood were used. The different types of cells along with antigen-antibody
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INTRODUCTION An important aspect of any biotechnological processes is the culture of animal cells in artificial media. These animal cells in culture are used in recombinant DNA technology‚ genetic manipulations and in a variety of industrial processes. Now-a -days it has become possible to use the cell and tissue culture in the areas of research which have a potential for economic value and commercialization. The animal cell cultures are being extensively used in production of vaccines‚ monoclonal
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Student’s Name: Marylene Mosburg Date: 11/31/2014 BIO169 Case_10: Nicholas’s Mysterious Suntan This case study presents a clinical situation related to the endocrine system. The questions at the each segment of the case will test your understanding of the endocrine system. Nicholas T.‚ age 32‚ is a busy systems analyst for a computer manufacturer. Working his way up the corporate ladder‚ he travels extensively‚ putting in many hours without much sleep. Nicholas has a tendency to be overweight
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Chronic Alcoholism (started drinking half gallon of tuba every day at the age of 16‚ beer and redhorse 2-3 times a week) Chronic Alcoholism (started drinking half gallon of tuba every day at the age of 16‚ beer and redhorse 2-3 times a week) Liver Cirrhosis Liver Cirrhosis Risk Factors * Chronic alcoholism * Smoking * Diet Malnutrition Risk Factors * Chronic alcoholism * Smoking * Diet Malnutrition Predisposing Factors * Age > 45 y/o * Gender (more common in
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interferon therapy) (Group 2) had significantly elevated serum levels of IL-10 (Th2 marker) and significantly decreased serum levels of IL-2 (Th1 marker)compared with HCV patients only (Group I). Also‚ there is a highly significantly elevated serum level of IL 10 between the two groups than control subjects‚ and also highly significant decrease in levels of IL-2 between the first two groups and the third group (control group).This increase in serum IL-10 in patients with HCV ‚ infection is in agreement
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oil blend‚ which were somewhat better than the digestibility coefficient of conventional vanaspati (93.6%). Feeding experiments for three months showed comparable results in terms of serum lipid profiles. The blended products significantly increased the total cholesterol level but not the free cholesterol level in serum and liver of rats when compared with those of the conventional vanaspati group of rats. JAOCS 73‚ 617-622 (1996)‚ There are two distinct methodologies for making zerotrans vanaspati-like
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Handbook of Pathophysiology (January 15‚ 2001): by Springhouse Corporation‚ With 13 Contributors‚ Springhouse By OkDoKeY Handbook of Pathophysiology Contents Staff Contributors Foreword 1 FUNDAMENTALS OF PATHOPHYSIOLOGY 2 CANCER 3 INFECTION 4 GENETICS 5 FLUIDS AND ELECTROLYTES 6 CARDIOVASCULAR SYSTEM 7 RESPIRATORY SYSTEM 8 NERVOUS SYSTEM 9 GASTROINTESTINAL SYSTEM Pathophysiology in color Understanding Asthma Understanding Cancer Understanding Osteoporosis Understanding Ulcers 10 MUSCULOSKELETAL
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Hi my name is Bryson Yelle‚ and I am on the affirmative side of this debate and I shall be explaining why human experimentation is ethical. Human experimentation Over the years has saved us‚ for example the polio vaccine. The polioVirus happened around the early 1940s to the late 1950s‚ and many of thousands were dying because of it until a man named Jonas salk started his human experimentation in 1952 and released it to the US in 1955. His experimentation killed at around 11 people‚ but saved thousands
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