PURIFICATION OF LACTATE DEHYDROGENASE FROM CHICKEN MUSCLE TISSUE Abstract The enzyme lactate dehydrogenase (LDH) catalyzes the last step of anaerobic glycolysis that is important for the normal function of the body. Purification of LDH is essential to understand its structure and function. The purpose of this experiment was to extract and purify LDH enzyme from chicken muscle tissue using a variety of various. Analytical methods such as activity and protein assay were employed to determine
Premium Enzyme Protein
of organic bone matrix‚ osteoid‚ which does not become calcified (Guyton and Hall‚ 2001). Consequently‚ the newly formed‚ uncalcified osteoid gradually takes the place of other bone that is being reabsorbed. During the later stages of rickets‚ the serum calcium level falls precipitously‚ and tetany (neuromuscular spasm) develops. In infants and young children‚ a long-standing calcium intake deficiency‚ in association with suboptimal vitamin D exposure‚ can produce rickets. Indeed‚ in experimental
Premium Vitamin D
PHILIPPINE WOMEN’S UNIVERSITY SCHOOL OF DISTANCE EDUCATION MASTER OF ARTS IN NURSING SISTER. CALLISTA ROY’S “ADAPTATION MODEL” IN SAN LAZARO HOSPITAL NURSING SERVICE BY RONALD T. REYES‚ RN ADAPTATION MODEL IN NURSING SERVICE Assessing patients knowledge to her condition and to what a patient can do to cooperate and the availability of the resources‚ a nurse can have the all needed idea to what may be necessary intervention that is adequate to be implemented the patients care
Premium
result for two factions instead of one that decides their future. As for Tris‚ her Divergence is special because she received an aptitude for three factions‚ making her a target for the Erudite faction leader Jeanine Mathews to formulate the right serum‚ “I have determined that you are one of the strongest Divergent… If I am to develop a simulation that [isn’t] thwarted by the Divergent mind‚ I must study the strongest” (Roth 328). Being Divergent is a dangerous thing to be also because it makes them
Premium
Thyroid Stimulating Hormone (TSH) in the blood serum. 1. The normal range for total amount of T4 in serum is 0.05 ÷0.14 mg/L. For each patient‚ type in their serum T4 concentration and indicate if it is lower‚ within‚ or higher than the normal range. a. Patient with primary hypothyroidism b. Patient with secondary hypothyroidism c. Patient with hyperthyroidism 2. The normal range for serum TSH is 0.35 ÷ 4.5 mIU/L . For each patient‚ type in her serum TSH concentration and indicate if it is lower
Premium Hypothyroidism Thyroid Thyroid hormone
dominant over the recessive i. The antigen with the Rh factor is determined by D as dominant or d as recessive‚ with the Rh positive factor as dominant and the Rh negative factor as recessive. In the ABO blood test‚ synthetic blood samples and synthetic serum of Anti-A‚ Anti-B‚ and Anti-Rh are mixed respectively into a blood typing slide. If agglutination occurs as the samples are mixed‚ then the subject’s blood type is the respective A‚ B‚ or Rh factor. The father with the most closely related alleles
Premium Blood type Blood Allele
least 4 dropper vial 3 blood samples Synthetic Anti – A Synthetic Anti –B Synthetic Anti – Rh serum 3 tooth picks (to stir) Blood typing side Methods Using the dropper vial‚ place a drop of the first synthetic blood sample in each well of the blood typing slide. Then add a drop of Anti-A to one well labeled A. Next add a drop of Anti-B to another well labeled B. Next we added a drop of Anti-Rh serum to the last well labeled‚ RH. Using different color mixing sticks for each well. If a film remains
Premium Blood Karl Landsteiner Blood type
Serum creatinine is an important marker of renal health as it is an easily measured byproduct of muscle metabolism that is excreted unchanged by the kidneys. Creatinine itself is produced by a biological system include creatine‚ phosphocreatine or creatine phosphate‚ and adenosine triphosphate “ATP”. Creatine is formed mainly in the liver from the methylation of glycocyamine by S-adenosyl methionine. It is then carried through blood to the other organs‚ muscle‚ and brain‚ where‚ through phosphorylation
Premium Hypertension Blood Blood pressure
Natalie Salaverria 01/21/11 LAB EXERCISE I After the first exercise : Simulating Simple diffusion Which Materials diffused from the left beaker to the right beaker? NaCl ‚ Urea ‚ Glucose Which did not ? Albumin Why ? Albumin’s Composition or charge was too great to diffuse through the membrane. After the 2nd activity : Simulating Dialysis What happens to the urea concentration in the left beaker (the Patient)? It diffused to the right beaker Why does this occur? The excess
Premium Osmosis Diffusion
Introduction On Saturday morning of the 24th of October‚ there was report by an employee of a homicide incident on 4011 N. Central Ave‚ Phoenix‚ AZ. University Center building on the 3rd floor of Arizona State University. The victim was describe as a 24 year old Caucasian female‚ by the name of Kelly; who works as a programs coordinator for Arizona State University. The victim was working late hours as usual on a Friday night‚ she was on her office cubical. The body was found laying on the floor
Premium Blood Blood type Fingerprint