IMMUNOCHEMICAL TECHNIQUES: Immunochemistry is an advanced area of immunology. It deals with the chemical components and chemistry (chemical reactions) of immunological phenomena that is of antibody and antigen. Immunochemical methods are processes utilizing the highly specific affinity of an antibody for its antigen. It detects the distribution of a given protein or antigen in tissues or cells. The methods used for the immunochemical analysis are called Immunochemical techniques. Characteristics/Advantages
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Isolation of Acetyleugenol (Clove Oil) from Cloves Purpose In this experiment‚ you will obtain the essential oil from cloves by steam distillation. You will then separate a component (acetyleugenol) from the oil by liquid-liquid extraction. Finally‚ you will isolate the acetyleugenol by using a rotovap for reduced pressure distillation‚ which will remove the organic solvent (dichloromethane). Background Whole cloves contain between 14% and 20% by weight of essential oils‚ but
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Separation and Purification of Organic Compounds Codilla‚ Christine‚ Cristobal‚ Marian‚ Dionisio‚ Jermaine‚ Dumaquita‚ Vanessa‚ Edrosolo‚ Lyka‚ Esquivel‚ Christine1 1University of Centro Escolar‚ Makati Date Performed: December 5‚ 2012 Date Submitted: December 17‚ 2012 Abstract The distillation process was successfully done because of the separation of water from the acetone. When the distillate was lighted with the matchstick it produced flame which indicates that there was more presence
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Analytical techniques Introduction * The analysis technique chosen is gas chromatography‚ I’m going to talk about it and I am elected chromatography studies to identify and quantify known materials do. * The principles of gas chromatography * The functions of gas chromatography * Theories of instruments and techniques etc. PRINCIPLES Gas chromatography (GC) was first demonstrated for analysis of liquid in the classic paper by james and martin (1952). It was quickly recognised
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Assignment 2: Answer Sheet (LSM 1401 Semester 2 AY2011/12) Nucleic Acid Virtual Laboratories |Name | | |Matriculation number | | |A. DNA Extraction Virtual Lab [2 marks]
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Extraction of Caffeine from Tealeaves Perez‚ Albert Solomon K.*; Quiambao‚ Marie Angela C;.Pascua‚ Hanna Harriette R.; Patricio‚ Maria Roxanne DC.; 2-Chemistry‚ Department of Chemistry‚ College of Science University of Santo Tomas España St.‚ 1015‚ Manila Abstract Caffeine is soluble in both water and organic solvents‚ solid-liquid extraction and liquid-liquid extraction were used in the experimentation process. Caffeine was extracted using hot water‚ however‚ due to its
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Extraction of Caffeine from Tea Leaves Introduction Caffeine is soluble in boiling water and as a result it is easily extracted from tea bags by steeping in hot water. This process leaves behind the water insoluble portions of the tea bag. However‚ water extracts more than just caffeine‚ so a final separation is done with an organic solvent that will dissolve primarily caffeine. The organic solvent used in this experiment is Dichloromethane (CH₂Cl₂). Dichloromethane is less polar than water
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EXPERIMENT 2 ACID DISSOCIATION CONSTANT OF AN INDICATOR DYE OBJECTIVES Using spectrophotometric method: determine the wavelengths at which the acid and base forms of the dye in aqueous medium exhibit maximum absorption; determine the molar absorptivities of the acid and base forms of the dye and estimate an unknown concentration of the dye in solution using the Beer-Lambert’s Law; and determine the acid dissociation constant of the indicator dye. THEORY The absorption or reflection of
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Acetylsalicylic Acid (Aspirin) Synthesis Telow‚ AJV Sumicad‚ CJ‚ Tavanlar‚ EMMT‚ Chem 40.1‚ Institute of Chemistry‚ University of the Philippines Los Baños I. Introduction Organic synthesis is the process where a desired organic compound is constructed or prepared from commercially available materials. The objective of organic synthesis is to design the simplest synthetic routes to a molecule. Aspirin‚ also known as acetylsalicylic acid is as salicylate drug often used as analgesic
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Sam Chu (Jacob Gorman and Tyler Kui) Lab #1: Separation of a Mixture Mr. Mejia 10/3/14 Separation of a Mixture Lab Report Abstract The purpose of the experiment was to separate an initial heterogeneous mixture composed of 5.00 grams of salt‚ 2.00 grams of sand‚ 50.0 mL of water‚ 15.00 grams of pebbles‚ and 1.00 gram of iron filings‚ and leave as much salt as possible remaining. Using separation techniques including magnetizing‚ evaporation‚ filtration‚ etc. the heterogeneous mixture was thoroughly
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