EXTRACTION OF DNA FROM ONIONS ABSTRACT The purpose of the experiment was to experience firsthand the isolation of DNA form a plant tissue without destroying its structure and sequence. A white onion was used for the experiment. After several processes‚ DNA isolate was the visible result. Different chemical tests were performed on the DNA isolate‚ namely: Dische Test‚ Murexide Test‚ Wheeler-Johnson test and Test for Phosphate. Visible results were then noted. INTRODUCTION DNA (deoxyribonucleic
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Case Vignettes in Acid-Base Balance Due Saturday‚ March 16 th Choose three of the four vignettes and BRIEFLY answer the questions that follow. Normal Levels of Substances in the Arterial Blood: pH 7.40 + 0.05 pCO2 (partial pressure of carbon dioxide) 40 mm Hg pO2 (partial pressure of oxygen) 90 - 100 mm Hg Hemoglobin - O2 saturation 94 - 100 % [HCO3-] 24 meq / liter Vignette #1: A 14-year-old girl with cystic fibrosis has complained of an increased cough productive of green sputum over
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Lab 1: Acid/Base Properties of an Antimicrobial Agent 09/03/13 Purpose The purpose was to determine the acid and base properties of the antimicrobial agent sodium benzoate. Theory Sodium has mainly been used in food processing to avoid growth of bacteria and harmful microorganisms. It is commonly used to preserve foods and beverages that have an acidic pH. Rather than benzoic acid‚ sodium benzoate is used because it is generally soluble in most aqueous solutions but benzoic acid is not
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4/2/14 Period: 1st Chemistry Sour Acids and Bitter Bases Purpose: The purpose of this lab was to observe the different reactions formed between various acids and bases with the aid of indicators. Equipment: 1. Safety goggles. 2. Droppers. 3. Red Litmus paper. 4. Blue Litmus paper. 5. pH paper. 6. Well plate. 7. Micro spatula. Materials: 1. Zinc. 2. Magnesium. 3. Iron. 4. Copper. 5. HCL. 6. HC₂H₃O₂. 7. NaOH. 8. Phenolphthalein. Procedure: Part A: 1. Add five drops
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Date Performed: December 17‚ 2013 Group No.: 8 Date Submitted: January 6‚ 2014 Experiment 4: Extraction and Chromatic Separation of Plant Pigments from Tomato Paste ABSTRACT In column chromatography a mixture is dissolved in a solvent and poured over a column of solid adsorbent then eluted with the same or a different solvent. This method is often used for preparative
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DNA EXTRACTION FROM KIWI ISGR SCIENCE Aalah Yousif‚ Ella Sobek April 27‚ 2012 Background All living organisms (plants‚ animals‚ and bacteria) are made up of cells. Any cell consists of many parts but the parts that play the key roles are the nucleus‚ cell membrane‚ and cytoplasm. The nucleus is like the “brain” of the cell (that also contains DNA). The cell membrane is like a wall that guards the cell. And the cytoplasm is a jelly-like substance which contains other smaller organelles like
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acetic acid present in commercial vinegar and the weight-to-volume percent of ammonia present in window cleaners. Acid-base titrations can be conducted using the above samples against standardized aqueous NaOH and standardized aqueous HCl solutions respectively. 2. Introduction: Acetic acid‚ commonly known as ethanoic acid CH3COOH‚ is found in commercial vinegar. It is a weak organic acid that gives vinegar its characteristic pungent smell‚ sour taste and slight acidity. Being an acid‚ vinegar
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Introduction Background Information To begin a discussion about acid-base titrations‚ we must first recall that there are several definitions of acids and bases. For the purpose of this exercise‚ we will consider the Arrhenius definition of acids and bases‚ in which an acid is a proton (H+) donor and a base produces hydroxide (OH-) in solution. When an acid reacts with a base‚ the products of this reaction are water and a salt. Note that salt here does not only mean table salt (NaCl)‚ but can refer
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Exercise 10: Acid/Base Balance Worksheet Assignment Due: Week 1 GRADE 94 Student instructions: Follow the step-by-step instructions for this exercise found in your text and record your answers in the spaces below. Submit this completed document by the assignment due date found in the Syllabus. Please make sure that your answers are typed in RED. Please type your Name and Student ID Number here: Paul M. Nannery 4006529 Respiratory Acidosis and Alkalosis Activity 1: Normal Breathing
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PART THREE Renal Acid-Base Balance 1 Acid • • • • An acid is when hydrogen ions accumulate in a solution. It becomes more acidic [H+] increases = more acidity CO2 is an example of an acid. HCl 2 H+ H+ ClH+ H+ ClH+ 7 ClpH ClCl- As concentration of hydrogen ions increases‚ pH drops Base • A base is chemical that will remove hydrogen ions from the solution • Bicarbonate is an example of a base. NaOH Na+ OH- H + ClH+ ClNa+ OHH+ ClH+ Cl- Na+ OHH+ ClNa+ OH- 2 7 pH Acids and basis neutralize
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