Genomes as Resources for Biocatalysis JON D. STEWART Department of Chemistry‚ University of Florida Gainesville‚ Florida 32611 I. II. III. IV. Introduction Yeast Dehydrogenase Gene Identification Expression and Isolation of Yeast Dehydrogenases Characterization of Yeast Dehydrogenases A. Results from Ethyl Acetoacetates B. Results from Higher Homologs C. Synthetic Applications V. Conclusions and Future Directions References 31 33 34 35 37 40 43 46 49 I. Introduction How well biocatalysis
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WTHESIS STATEMENT: “BIOCONVERSION OF WATER HYACINTH (EICHHORNIA CRASSIPES) TO ETHANOL.” 1. INTRODUCTION Water hyacinth (Eichhornia crassipes) is a free-floating or rooted in mud plant or weed which can totally cover fresh water lakes‚ harbors‚ and other essentially relatively occluded fresh water bodies. It is a fast growing permanent aquatic plant that are extensively scattered all throughout the world. This tropical plant can be the reason for the infestation over large areas of water resources
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COVENANT UNIVERSITY Department of Biological Sciences NAME: OBUEKWE CHUKWUEMEKA AZUBUIKE MAT NO: 12CQ014230 PROGRAMME: MICROBIOLOGY COURSE: MCB 212 (MYCOLOGY) TOPIC: THE IMPORTANCE OF FUNGI IN; MEDICINE INDUSTRY THE ENVIRONMENT {DR OLASEINDE G.} INTRODUCTION Fungi are tremendously important to human society and the planet we live on
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Cellular respiration is defined as an enzyme mediated process in which organic compounds such as glucose is broken down into simpler products with the release of energy (Duka‚ Diaz and Villa‚ 2009). It is a series of metabolic processes and oxidation-reduction reactions. Oxidation of substrates‚ such as glucose‚ is a fundamental part of cellular respiration (Mader‚ 2009). As a catabolic process‚ it may or may not require the presence of oxygen. The process that requires oxygen is called aerobic
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yeast species that dominated were Zygosaccharomyces bailii‚ Saccharomyces ludwigii‚ Schizosaccharomyces pombe and Saccharomyces cerevisiae; others varyingly include‚ Kloeckera apiculata‚ ‚ Brettanomyces bruxellensis‚ B. lambicus‚ B. custersii‚ Candida stellata ‚ Pichia fermentans and Torulaspora delbrueckii (Mayser et al.‚ 1995; Liu et al.‚ 1996; Balentine et al.‚ 1997; Markov et al.‚ 2001; Ramadani and Abulreesh‚ 2010)‚ Saccharomyces bisporus (Markov et al.‚ 2001) and B. claussenii (Jayabalan
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Yeast which is also known as Saccharomyces Cerevisiae is a unicellular eukaryotic Fungi means that is made up of one cell with a nucleus(“What is Yeast”).Yeast is a very practical product that is used in mainy way like when baking Yeast helps raise the dough and also yeast is used in the process of making wine.The Reason why yeast was chosen to was to see how yeast can metabolize different sugars and how much Co2 they release when when metabolizing.To test the yeast at the lab we used 4 different
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fermentative methods using sugar as a starting material. In the latter method‚ Saccharomyces cerevisiae and Candida utilis are currently used to produce glutathione on an industrial scale. At the molecular level‚ the genes gshA and gshB‚ which encode the enzymes γglutamylcysteine synthetase and glutathione synthetase‚ respectively‚ have been cloned from Escherichia coli and over-expressed in E. coli‚ S. cerevisiae‚ and Lactococcus lactis. It is anticipated that‚ with the design and/or discovery
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Lab 4: Fungi Non-filamentous forms — Single-celled Yeasts Do an Internet search for a microscopic image of baker’s yeast‚ Saccharomyces cerevisiae (Ascomycota). Answer the following questions: 1. Do the yeast cells have a definite shape or is there considerable variation? Yeast cells seem to vary in shape‚ some look to be more oval and round‚ although. some slides did show pear and cylinder shapes. 2. Can you detect any subcellular structure? I cannot detect a clearly visible
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Solutions with Different Concentrations of Glucose Brandon Bosley BIO 121 11/19/2013 Introduction: In our lab this week we tried to see how different amounts of substrates affect our organism‚ yeast‚ in its fermentation process. Yeast (Saccharomyces cerevisiae) is an organism that is cultured for the cells themselves‚ as well as the end products that they produce during fermentation. Yeasts are commonly known for the ethanol fermentation due to their ability to produce ethanol for industrial purposes
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The cell cycle‚ or cell-division cycle‚ is the series of events that take place in a cell leading to its division and duplication that produces two daughter cells. In cells without a nucleus‚ the cell cycle occurs via a process termed binary fission. In cells with a nucleus‚ the cell cycle can be divided in three periods: interphase—during which the cell grows‚ accumulating nutrients needed for mitosis preparing it for cell division and duplicating its DNA—and the mitotic phase‚ during which the
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