Introduction Plasmids are circular‚ double stranded extrachromosomal DNA molecules that are found in bacteria which can self-replicate. They are naturally occurring DNA molecules advantageous to the host bacterium by carrying genes which specify metabolic capacities. (Garrett et al.‚ 2010) Besides‚ plasmids exist in a wide variety of sizes from a few thousands to hundreds of thousands of base pairs. Many plasmids have been engineered to serve as plasmids cloning vectors to carry genes. (Synder et
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course. The main focus of the experiment would be how the Restriction Endonucleases cleave the strands of DNA. For this experiment‚ pBR322 was the specimen to use. Restriction Endonucleases work by cleaving the sugar phosphate backbone of specific DNA sites. Restriction enzymes that have been isolated from bacteria have a defensive role. This idea is illustrated when an attacking foreign cell DNA is trying to alter the bacteria; restriction enzymes cleave the DNA rendering it inert. The second
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Wintersemester / page 1 Experiment 22 Isolation of plasmid-DNA from bacteria and PCR Advisor Konrad Egli: kegli@botinst.unizh.ch Reading Chapters in BBOM 10th: 10.8 BBOM : Madigan M.T.‚ J.M. Martinko and J. Parker: "Brock - Biology of Microorganisms"‚ 10th Edition (2003)‚ Prentice Hall. Objectives Background • Isolation of plasmid-DNA from different bacteria clones • Handling of bacteria clones • PCR-experiment The typical plasmid is a circular double-standed DNA molecule less
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uses of plasmids in G.M. experimentation. Plasmids are extrachromosomal genetic elements found in a variety of bacterial species. They are double stranded; autonomously replicating‚ supercoiled‚ covalently closed circular (ccc) DNA molecules that range in size from 1 kb to greater than 200 kb. Often‚ plasmids contain genes coding for enzymes that‚ under certain circumstances‚ are advantageous to the bacterial host (Table 1). Table 1. Some of the phenotypes conferred by different plasmids that
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I. Title Identification of an Unknown Plasmid In this experiment‚ we determined the phenotypic capability of an unknown plasmid along with its size. With the use of gel electrophoresis‚ we analyzed the gel photograph by using a standard DNA marker‚ Lambda HindIII‚ and came to a conclusion based on our results. II. Abstract Two experiments were done to identify an unknown plasmid. The success of these experiments came from the use of modern day technology involving gel electrophoresis
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Isolation of plasmid DNA and analysis of isolated plasmid Introduction: A plasmid is an autonomously replicating extra-chromosomal genetic element. In other words‚ this is a DNA molecule external to the bacterial chromosome that is able to replicate on its own and distribute its daughter molecules to daughter cells. You have successfully cloned a fragment of chromosomal DNA containing a tetracycline resistance cassette into a plasmid (pET11a). To this end you have (1) isolated total chromosomal
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DIGESTED CASES Macalintal vs COMELEC [G.R. No. 157013. July 10‚ 2003] Facts: A petition for certiorari and prohibition filed by Romulo Macalintal‚ a memer of the Philippine Bar‚ seeking a declaration that certain provisions of RA 9189 (The Overseas Absentee Voting Act of 2003) suffer from constitutional infirmity. He claimed that he has actual and material legal interest in the subject matter of this case in seeing to it that public funds are properly and lawfully used and appropriated‚ petitioner
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Kenneth Hampton | | |Restriction Enzymes: | |A study in Reactions and Mapping | | | |November 7‚ 2008 | ABSTRACT This experiment will study the
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ANG LADLAD v. COMELEC Facts: This is a Petition for Certiorari under Rule 65 of the Rules of Court‚ with an application for a writ of preliminary mandatory injunction‚ filed by Ang Ladlad LGBT Party (Ang Ladlad ) against the Resolutions of the COMELEC dated November 11‚ 2009 (the 1st Assailed Resolution) and December 16‚ 2009 (the 2nd Assailed Resolution).The case has its roots in the COMELEC’s refusal to accredit Ang Ladlad as a party-list organization under RA 7941‚ otherwise known as the
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Republic of the Philippines SUPREME COURT Manila SECOND DIVISION G.R. No. 115758 March 19‚ 2002 ELIDAD C. KHO‚ doing business under the name and style of KEC COSMETICS LABORATORY‚ petitioner‚ vs. HON. COURT OF APPEALS‚ SUMMERVILLE GENERAL MERCHANDISING and COMPANY‚ and ANG TIAM CHAY‚ respondents. DE LEON‚ JR.‚ J.: Before us is a petition for review on certiorari of the Decision1 dated May 24‚ 1993 of the Court of Appeals setting aside and declaring as null and void the Orders2 dated
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