Title : FOOD TESTS Aim : To determine whether given unknown solutions A‚B‚C‚D‚E‚F and G contain Reducing/NonReducing Sugars‚ Lipids‚ Proteins or Starch. Apparatus/Materials: - Bunsen burner - Solutions A‚ B‚ C‚ D‚ E‚ F and G (unknown) - Measuring Syringes - Stirring Rod - Beakers - Test tubes (7)plus holder and tray - Copper sulphate solution (CuSO4) - White dropping ray - tripod stand and mesh -stopwatch - Biuret͛s solution - Sodium Hydroxide solution (NaOH) (or Potassium Hydroxide
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Experiment 2: Food Tests Objective * To study the presence of reducing sugars. * To study the presence of protein. Introduction In this experiment‚ glucose‚ maltose‚ lactose and sucrose are used for testing reducing and non-reducing sugars. Glucose is monosaccharide while maltose‚ lactose and sucrose are disaccharides of carbohydrates. Monosaccharaides are the monomers which make up all other carbohydrates and cannot be broken into smaller molecules by hydrolysis. Disaccharides are
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Aim: To find out which samples consists of starch/ proteins/ reducing sugar/disaccharide out of 4 given samples A‚ B‚ C and D. Research Question: Out of the four given samples which sample consists of protein/ starch/reducing sugar/ disaccharide? What color changes will help to identify the contents of each sample when treated with iodine‚ biuret agent and benedicts solution? Theory and background information: What is a protein?1 Proteins are macromolecules‚ consisting of one or more chains
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Testing for reducing sugars‚ non-reducing sugars‚ starch‚ lipids and proteins in unknown substances: Our aim: was to find out which substances from our five samples have reducing sugars present to determine what they may be for example; they could be monosacchrides or disaccharides. This was carried out by using Benedict’s test. To find out which substances from our five samples were non-reducing sugars‚ since some disaccharides are reducing sugars and this would be carried out by using Benedict’s
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proteins‚ starches‚ and reducing sugars were present in the solutions we were provided with. The purpose of the lab was to allow us to have the opportunity to view the positive and negative reactions of the solutions we used‚ also to understand what that meant. The reagents we used allow us to detect whether or not there was a reducing sugar‚ starch‚ or protein present in the solutions. From what I knew previous to this experiment would be that if a substance contains a reducing sugar it will have a positive
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Determining the sugar concentration of food samples is very important especially in industries where quality control is monitored. One method to determine the sugar concentration of reducing sugars is by heating with 3‚5 dinitrosalicylic acid(DNS) which produce a red-brown product Miller(1959)The reaction is direct‚thus the method is preferred over the Benedict’s test method. The concentration of the coloured complex can be determined with the spectrophotometer at Absorbance 540.The sugar concentration
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Title: Food Tests Aim: To test an unknown substance for various food substances. Apparatus/Materials: * Unknown Solution X * NaOH * Iodine * Ethanol * Copper Sulphate * Benedict’s Solution * HCl * Cold Water * Syringe * Beakers * Bunsen burner * Tripod stand * Test tubes * Dropper Introduction: This Lab shows the detection of different food sources using different tests. Some of the food tests carried out was Benedict’s test‚ the
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TRIPLE SUGAR-IRON AGAR TEST Triple sugar-iron (TSI) agar test- designed to differentiate among the different groups or genera of the Enterobacteriaceae‚ which are all gram-negative bacilli capable of fermenting glucose with the production of acid a. Differentiation is made on the basis of differences in carbohydrate fermentation patterns and hydrogen sulfide production. To facilitate observation of carbohydrate utilization patterns- TSI agar slants contain lactose and sucrose (1%) concentrations
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Executive Summary Obesity is one of the major global issues worldwide that has significant influence on the society’s health of the population. One of the main causes of obesity is the high sugar daily intake and the foods containing high calories in large amounts. Obesity has significant effects to your body when you have obesity as it not only just impacts the overall appearance of yourself but inside your body within the organs are also affected. The organs will be covered in a layer of fat as
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1. Aim: To compare the reducing sugar content of dry raisin and fresh grape 2. Introduction: i) Principle of Benedict’s test Reducing sugar can reduce Cu2+ into Cu+ and form a bricked-red precipitate (copper(I) oxide) which is insoluble in water. ii) Use of Benedict’s test Benedict’s test can show the presence of reducing sugar (all monosaccharide and disaccharide‚ except sucrose). Add Benedict’s solution into food samples (solution). Then mix them up and put into a water bath for 5
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