water and Methylene blue solution and leave it undisturbed. The blue solution turned clear and when we added the yellow food coloring it turned an orange color. The solution then turned dark blue once the solution was shaken vigorously. This reaction occurred because the oxygen oxidized the methylene blue.In the oxidized state the methylene blue shows a deep blue color this corresponds with what happened first in our lab when the materials where mixed. In the reduced state methylene blue turned to
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The methylene blue staining procedure is used to measure yeast viability based on the assumption that the methylene blue will enter the cells and be broken down by living yeast cells that produce the enzymes which breaks down methylene blue‚ leaving the cells colourless. The non- viable cells do not produce this enzyme (or enzymes) and as such the methylene blue that enters the cells are undegraded causing the cells to remain coloured (the oxidized form concentrates intracellularly). The coloured
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Bio coursework Methylene blue Yeast cells explanation of respiration hence colour change etc Low temp colour change should be visible as the yeast cells are not necessarily dead‚ just inactive. Activity increases from 20-45 c High rate around 30-40 Starts to slow down basically enzyme curve see bio 1 100 degrees will kill all cells Do a few preliminary keep working down until first blue solution appears in unit of ten Then work to find degree. If more accuracy then half
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Independent University‚ Bangladesh Admission Test (Sample) Math-Physics (Engineering) Total Marks: 50 Name Instructions: a) Turn-off all mobile communication devices (cell-phones‚ PDAs‚ laptops‚ etc.) b) There are 25 (twenty five) problems. You have to attempt all of them. c) Each problem has 5 (five) possible answers. Choose the correct answer‚ and fill the appropriate oval on the answer sheet. d) There is no negative marking. e) Do not fill more than one oval for any problem. f) It is preferable
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As Thomas Graham (1831-1833) found out on his study that gases of different nature diffuse with each other‚ it is a challenge as to how true it is to solids. The effect of molecular weight on the rate of diffusion was assessed using Methylene Blue (320 g/mol) and Potassium Permanganate (158 g/mol). This subjects have varying molecular weight‚ one higher than the other. It is important to note whatvis Molecular Weight. MW is the sum of the mass of each constituent atom multiplied by the
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3A Task 1 Aim: the aim of the experiment is to find the best temperature to ferment yeast at. Hypothesis: the yeast will ferment the best at 60 degrees Celsius. Independent variable: the temperature of the water the yeast is put in to ferment. Dependent variable. The amount of air bubbles the yeast produces. Controlled variable: the amount of yeast and glucose in each syringe. Uncontrolled variables: human error in counting. Materials : Plastic soft drink bottle cut to size Marking pen
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In this lab my group and I tested to see how yeast would react to different substances. Yeast is a fungi that releases carbon dioxide which can be used in the process of bread making. During this lab we used sugar‚ warm water‚ cornstarch‚ yeast‚ a gas sensor‚ plastic cylinders‚ and a laptop. The process of this lab was to create cellular respiration‚ which is what cells do to break up sugars into a form that the cell can use as energy. When yeast and a certain substance were combined‚ this substance
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October 12th 2014! The Effect of Glucose on The Cellular Respiration of Yeast! Purpose: ! The purpose of this lab is to determine if the quantity glucose solution will increase or decrease the rate at which cellular respiration occurs at within the tested 20% yeast suspension.! Question:! What are the effects of increasing or decreasing the quantity of 0.06mol/L glucose solution on the cellular respiration within the tested yeast molecules?! Hypothesis: ! When the quantity of glucose solution is increased
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Sucrose Concentration On Cell Respiration In Yeast Abstract This lab investigates the effects of Sucrose concentration on cell respiration in yeast. Yeast produces ethyl alcohol and CO2 as a byproduct of anaerobic cellular respiration‚ so we measured the rate of cellular respiration by the amount of CO2 produced per minute. The results show a trend wherein increased concentrations of sucrose increase the rate of cellular respiration. Introduction All living cells require energy in order to
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Abstract The cytoplasm and extracellular environment of the cell are aqueous solutions. They are primarily composed of water (the solvent)‚ and a variety of dissolved solutes‚ such as sugars‚ amino acids‚ and ions. The plasma membrane of the cell is selectively permeable‚ allowing water to freely pass through‚ but regulating the movement of solutes. Water and some dissolved solutes move via passive diffusion through the plasma membrane. Diffusion is a process where molecules move from an area of
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