Report of Experiment 1 Purification and Identification of An Unknown Mixture Aim To separate a mixture of a neutral (ester‚ alcohol‚ ketone‚ aldehyde or ether) and an acidic compound (phenol or carboxylic acid) or a neutral and a basic compound (amine)‚ and to identify the unknown mixture by physical (M.P. or B.P.) and spectroscopic methods (NMR‚ IR and MS). Method The mixture (sample number: 27) was dissolved in 200 mL of CH2Cl2 and then extracted with 10% NaOH. The acid was removed
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LAB REPORT FOR EXPERIMENT #2: PURIFICATION OF ACETANILIDE BY RECRYSTALLIZATION Your name TA’s name Your Partner’s name Lab Section OBSERVATIONS: A. SELECTING A RECRYSTALLIZATION SOLVENT | |Solubility Test (cold) |Solubility Test (hot) | |Water |insoluble |soluble | |pet ether |insoluble
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Lab 4. Volumetric Determination of Impure Sodium Carbonate (Na2CO3) Introduction: To determine the total amount of carbonate in unrefined sodium carbonate‚ soda ash‚ a titration is done using a standardized solution of HCl. Aqueous HCl is a strong acid and therefore almost completely disassociates into H+ and CL-. Therefore‚ when HCl is used in a titration‚ the H+ is the titrant. Carbonate in aqueous solution is able to accept a proton‚ i.e. it acts as a base. When carbonate accepts the H+ a bicarbonate
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1ST SIX WEEKS PERIOD Unit A Chapter 1 Lesson 2 Classifying Plants Modeling Water Transport Material: * small sponge‚ * pie tin‚ * water‚ * glass‚ * red food coloring‚ * celery stalk. Procedure 1. Pour a cup of water into the pie tin and the glass. Add food coloring to the glass. 2. Have a student place the sponge in the pie tin and the celery stalk in the glass. 3. After 24 hours‚ ask students how the sponge and celery model water transport systems in
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The Separation and Purification of Organic Compounds Sunshine Marie Introduction Each year‚ many thousands of new or unknown organic compounds are prepared or extracted from natural sources such as plants‚ fungi and animals. In order to identify an unknown organic compound or the components of a mixture of organic compounds‚ it is necessary firstly to separate and purify the compounds. At any given temperature‚ a certain number of molecules of a substance in the liquid state have sufficient
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Skills: Planning and Design Topic: Pure and Impure Matter Date: January 13‚ 2012 Problem: Troy was told that the presence of an impurity raises the boiling point of water. Troy wanted to carry out an experiment with salt water and distilled water to determine which would have a higher boiling point. Suggest a plan and design for this lab. Hypothesis: The salt water will have a higher boiling point than the distilled water. Variables: Controlled: the amount of distilled water and salt. Manipulated:
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Tacula‚ Jassy Camille D.S. Activity 11 Acids and Bases I. Objectives * To identify some acids and some bases which are used in common household products. * To construct an operational definition of an acid and a base‚ using the characteristic properties of those substances. II. Procedure A. For acids * Place 2ml each of dilute acetic acid‚ hydrochloric acid‚ sulfuric acid‚ carbonated drink (colorless) and calamansi juice extract in
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Water purification is the process of removing undesirable chemicals‚ biological contaminants‚ suspended solids and gases from contaminated water. The goal is to produce water fit for a specific purpose. Most water is purified for human consumption (drinking water)‚ but water purification may also be designed for a variety of other purposes‚ including meeting the requirements of medical‚ pharmacological‚ chemical and industrial applications. In general the methods used include physical processes such
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Purification of solids Aim: To separate a mixture of carboxylic acid and a neutral substance by treatment with aqueous sodium hydroxide and purifying the carboxylic acid‚ measuring the melting points of the neutral and the acid components. METHOD: A mixture of( 5g) Carboxylic acid and neutral compound were separated by adding 2 mol dm-3 sodium hydroxide (25cm3) to the mixture‚ which separated the water soluble sodium from the water insoluble neutral component‚ by filtration. The solid at the
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Introduction Protein purification is the series of processes to isolate a single type of protein from a complex mixture. This is vital to extract and characterize the protein of interest. However‚ before doing so‚ it is important to release the protein from the subcellular organelles. This step is also known as homogenization. This step can be done with the use of blender. As the solution was homogenized‚ it may undergo saltation or acidation to remove impurities such as calcium anions. Hexane
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