The Expression and Purification of Recombinant Green Fluorescent Protein (rGFP) From E. coli strain‚ BL21(DE3)‚ Using Ni2+-Agarose Affinity Chromatography Abstract: The purpose of these series of experiments was to express and purify recombinant Green Fluorescent Protein (rGFP) from the E. coli strain‚ BL21(DE3) by beginning with its purification via a Ni2+-agarose affinity chromatography column. The His6 tag of the rGFP bound to the Ni2+-agarose column and washes and elutions were obtained
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Introduction ESTERS are compounds derived from the reaction of a organic acid with an alcohol. Acid + Phenol/alcohol --> ester + water R--C=O + R’--OH ----> R--C=O + H2O | | C-OH C-O-R’ Esters are the compounds that give fruits their characteristic flavours and odours. ie. methyl salycilate is "Oil of Wintergreen". http://www.petrik.com/PUBLIC/library/misc/glossary_of_org_chem.htm#ESTERS Aim To synthesise the ester 3-methylbutyl ethanoate( isopentyl acetate) and to identify the product
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Christy Chan (5) BIOLOGY LAB REPORT Investigation of the effect of metal ions on enzyme activity Objective To study the effect of two heavy metal ions‚ lead (II) ions and silver ions on the enzyme activity of invertase. Introduction Hypothesis: The rate of enzyme reaction of invertase decreases and the reaction eventually stops as the heavy metal ions inhibit the enzyme reactions. Biological principle: Heavy metal ions are non-competitive inhibitors. They do not compete with the substrates
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ABSTRACT Separation and identification of components of a mixture was determined by using Chromatography. The components of a mixture have different affinities for a stationary phase as well as different affinities for a mobile phase. INTRODUCTION An extremely common technique by using two chemicals and filter paper can give you sufficient information. A well-done Chromatography of dyes will provide you with enough data to determine if a liquid mixture is heterogeneous or homogenous . In a learning
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Protein Essay- Erin Keegan What are proteins? A polypeptide is a chain of monomers joined up to make a polymer. Proteins are organic polypeptides made up of a chain of amino acid residues- they’re residues because they lose a water molecule. The amino acids (monomers) join up by forming peptide bonds. When two amino acids join together‚ they become a dipeptide. This happens when the carboxyl group of an amino acid and the amino group of another amino acid link up through a condensation reaction (which
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Marketing II – Group Assignment Waters Chromatography Division: US field Sales Submitted by: Group 5‚Section D ArkayanBagchi (2010PGP063) Harveer Singh (2010PGP449) Kaushlendra Singh (2010PGP150) Krishna Kishore Burugula (2010PGP163) Loveneesh Solanki (2010PGP170) RohanWagh (2010PGP311) Sameer Morey (2010PGP325) Protagonist The cases does not give any specific issues to be solved by any
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Abstract This experiment aimed to study the effect of various denaturants on albumin and casein protein extracts through viscosity measurements. 5 mL samples of native and denatured protein solutions were prepared‚ using -mercaptoethanol‚ urea and SDS as denaturants for albumin‚ and NaOH‚ NaCL‚ HCL‚ -mercaptoethanol‚ urea and SDS for casein. 5 mL blank solutions for each denaturant used were also prepared. The viscosity of the solutions were determined using Ostwald viscometer. ________
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from the exchange of positive and negative ions between reacting solutions of two ionic compounds. Thus‚ in this example the precipitate must be either silver chloride‚ AgCl or sodium nitrate‚ NaNO3. Sodium nitrate dissolves readily in water and is therefore soluble. Thus we can conclude that silver chloride is the insoluble precipitate. We can represent the formation of this precipitate by a net ionic equation: Ag+ +NO3 ̄ +Na++Cl ̄→AgCl+NO3 ̄+Na+ Net: Ag+ (aq)+Cl ̄ (aq)→AgCl(s) Ions present in
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PAPER and COLUMN CHROMATOGRAPHY REPORT 1. 2. The unknown code is an amino acid‚ Glycine. To justify the reason is because the retention factor is the same exact number as the Glycine. The data of the unknown shows everything to be exact with the data of the Glycine 3. The mobile phase is the more polar during the capillary action of the experiment. As soon as the paper touches the mobile phase‚ the solvent rises to the amino acids. This is where you can find polarity of the amino acids.
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CONTENT INTRODUCTION WATER PURIFICATION BY NANOPARTICLES NANOPARTICLES IN WASTE WATER TREATMENT MATERIALS AND METHODS CONCLUSION REFERENCES INTRODUCTION WATER PURIFICATION: It is the process of removing undesirable chemicals‚ biological contaminants‚ suspended solids and gases from contaminated water. NEED: The goal of this process is to produce water fit for a specific purpose
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