BIOL 1F90 Expt. #1: Protein Quantification Student Name: Carlos Osorio Student ID: 5220710 Lab Section: 34 Date Experiment Performed: Sept. 26th‚ 2012 Lab Partners: K. Cloutier J. Yang ABSTRACT Protein concentration analysis is primarily done through an accepted form commonly referred to as the Bradford Protein Assay. The main purpose of this experiment was to observe and record the various protein samples’ absorbency values through the calibrated readings of a spectrophotometer
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April 22‚ 2015 Does protein help to build muscle? The latest craze is about protein and how it affects building muscles and building muscle strength. Today‚ there are many athletes that use it as a supplement. There are many protein supplements available that are claiming to help people who work out to build muscle faster. Protein is a structure within our bodies that consists of amino acids. The cells in our bodies need this to function properly. If we did not have protein‚ our body’s functions
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Separation of Proteins and Mass Analysis Using SDS PAGE Biology 00-01L Abstract This experiment consisted of separating proteins into polypeptides using a method called SDS PAGE which is a type of electrophoresis. The polypeptides had different masses‚ so each polypeptide traveled a different distance and this was an essential part of the lab which demonstrated that there exists a relationship between the distance traveled by the protein and the mass of the protein. This relationship was graphed
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Test Biuret Test Reagents CuSO4 sol’n‚ diluted NaOH Test for Intact proteins and protein hydrlysates (at least tripeptide‚ 2 peptide bonds Result Pink to violet blue color Ninhyrin Test 1‚2‚3-indanetrione monohydrate or triketohydintene hydrate‚ ethanol Alpha- amino group (usually a general test for amino acids) Xanthoproteic Test Conc. HNO3‚ conc. NaOH For W‚F‚Y (aromatic except for H) Blue to blue-violet Oxidative decarboxylation color & deamination
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Determination of Protein Content Using Kjedahl and Titration 2.0 Introduction Proteins are polymers. They are the source of dietary amino acids and are used for growth and maintenance of living systems. They are costlier sources of energy compared to carbohydrates and fats and hence the human body utilizes proteins mainly for biosynthesis rather than as an energy source‚ though the energy yield is 5 kcal/g of protein. Twenty different types of amino acids occur naturally in proteins. Proteins differ
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BIURET FOOD TEST FOR PROTEINS molecules consisting of one or more chains of amino acids.Proteins are complex molecules that react differently to many compounds but are also fragile and cannot withstand high temperatures or strong acids and bases without degrading. The Biuret Method‚ or biuret test‚ is used to detect the presence of peptide bonds. It has this name because it reacts positively to the biuret molecule’s peptide bonds. Correctly evaluating the number of peptide bonds is a step towards
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PROTEIN Prior to completing the lab experiment‚ it was predicted that whipping cream‚ soy milk‚ and the flour solution would all contain proteins. Looking back at the collected observations it can be concluded that the hypothesized statements were all deemed accurate. However‚ corn syrup was the only substance that did not test positive for proteins. This abnormality was researched‚ and it is now clear why only three out of the four substances tested positive for proteins. To start‚ it was hypothesized
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October 2‚ 2012 Biochemistry I Circular Dichroism and Secondary Structure of Proteins Proteins are vital to an organism life; they are involved in nearly all cellular functions. It is an essential part of enzymes‚ the cellular membrane‚ active transport‚ protein synthesis and wound healing. Because one relies so heavily upon proteins and its function the structure of proteins is also very important. “The way a protein will fold over its self-determines how it interacts with other chemicals in its
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Proteins are the building blocks of our cells. The proteins that are in our body and ingested are broken down into amino acids and used throughout the body. Our bodies need protein to provide fuel to do the basic of functions. Protein helps to give energy and helps with muscles‚ bones‚ and cells growth. There are two different types of proteins. Complete protein provides all the amino acids that are needed within the body. Incomplete proteins provide some but not all of the necessary amino
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How Primary Structure Determines Protein Function Proteins are everywhere. As enzymes‚ they catalyse chemical reactions. There are many different types of proteins: carrier and channel proteins which transport substances‚ receptor proteins which are recognition site for antibodies and hormones‚ structural proteins which support cells and tissues‚ and hormones which transmit information. Proteins are big molecules with large molecular masses that are made up of many amino acids; they’re‚ essentially
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