2014). These proteins mostly consist of globulins (80%) and albumins (20%). The albumins contain protease inhibitors and lectins (Revilla 2015). Faba bean is rich in lysine (5.4-6.8%) and deficient in sulphur amino acids (0.6-1.0% methionine). Faba bean contains 51-68% of carbohydrates‚ including starch
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Aim To investigate the effect of different temperatures on the activity of rennin in milk Introduction Enzymes are globular protein‚ responsible for most of the chemical activities of living organisms. They are made up of long chains of amino acids containing carbon‚ hydrogen‚ oxygen and nitrogen (Gunsch‚ 2012). The role of enzyme is to act as catalysts‚ substances that speed up chemical reactions without being chemically altered during the process. The speeding up of chemical reactions is done
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Basic Reagents and Techniques of Molecular Biology Laboratory Protocols Ausubel F‚ Brent R‚ Kingston R‚ Moore D‚ Seidman J‚ Smith J‚ Struhl K. Current Protocols in molecular biology. John Wiley & Sons‚ New York‚ 1996. Sambrook J‚ Fritsch EF‚ Maniatis T. Molecular Cloning: a Laboratory Manual‚ 2nd ed.‚ Cold Spring Harbor Laboratory‚ Cold Spring Harbor‚ N.Y.‚ 1989. Basic Tools for DNA Manipulation and Molecular Genetic Analysis Enzymes Vectors Gel Electrophoresis PCR Southern blotting
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the rate of diffusion. 5. Why do you think that the urea was not able to diffuse through the 20 MWCO membrane? 6. Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. 7. Put the following in order from smallest to largest molecular weight: glucose‚ sodium chloride‚ albumin‚ and urea. ACTIVITY 2: Simulated Facilitated Diffusion Answers 8. True or False: The facilitated diffusion of glucose requires energy from ATP. 9. Which of the following
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adrenergic receptors. Adrenergic receptors are present in nearly all the peripheral tissues and in the central nervous system neurons. Three types of adrenergic receptors are present – alpha 1‚ alpha 2 and alpha 3(29). They belong to the cell surface G-protein coupled type of receptors. Alpha 2 receptors are subdivided into three subtypes alpha 2A‚ alpha 2B and alpha 2C(30).
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PCR consists of three steps: • Denaturation: Samples of DNA with the target sequence are heated to a reaction temperature of 94-96˚ C that causes DNA melting of the DNA template without denaturing the enzyme by disrupting the hydrogen bonds between complementary bases of the double helical DNA‚ yielding single-stranded DNA molecules(“How Is PCR (polymerase Chain Reaction) Done?”). DNA polymerase does not get degraded in such high temperatures since the DNA polymerase used in this reaction is thermostable
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widely among species. Factors causing these variances include: the type of protein; the proportion of protein‚ fat‚ and sugar; the levels of various vitamins and minerals; and the size of the butterfat globules‚ and the strength of the curd. On average‚ cow milk contains 3.4% protein‚ 3.6% fat‚ and 4.6% lactose‚ 0.7% minerals and supplies 66 kcal of energy per 100 grams. Bovine milk normally contains 30-35 grams of protein per liter. Of which‚ 80% is arranged in casein micelles. Figure 1. Model
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Introduction Enzymes are proteins that act as catalysts; thus allowing the many chemical reactions in life to occur. The purpose of an enzyme is to speed up the rate of a chemical reaction. Without enzymes‚ living organisms would be unable to function properly‚ since the releasing and storing of energy depend on the enzyme’s function (2). Through the process of catabolism‚ complex molecules within organic compounds are broken into simpler molecules. As a result of catabolic reactions‚ energy used
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Phosphatase | BCHM 405 | Aaron BANESEH 10307275 | Table of Contents 1 Table of Contents 2 2 Introduction 4 3 Experimental Procedures 5 3.1 Sample preparation 5 3.2 Initial Extraction of Acid Phosphatase 5 3.3 Determination of Protein Concentration by the Folin-Lowry Assay 5 3.4 Specific Activity Assay 6 3.4.1 Definition of Enzyme Unit and Specific Activity- 6 3.5 Determination of pH Optimum 6 3.6 Determination of Michaelis-Menten Constant 7 3.7 Ammonium Sulphate
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the 200 MWCO pore because it was small enough‚ having a molecular weight of 180.2 g/mol. Albumin was unable to pass through because it was far too large‚ having a molecular
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