We successfully adapted a commercially available protein detection assay for the quantiWcation of PEI. All experiments described below were performed with linear 25-kDa PEI (Polysciences‚ Warrington‚ PA‚ USA) that we routinely use for the transfection of mammalian cells [4]‚ but it was possible to quantitate other PEIs (diVerent molecular weights; branched) with this method (data not shown). The mixing of a PEI solution with the Advanced Protein Assay (APA) reagent (Cytoskeleton‚ Denver‚ CO‚ USA)
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Gas chromatography is the common method used for the biogasoline analysis. Biogasoline catalytic cracking process requires detailed qualitative and quantitative analysis of the hydrocarbon composition of its oil and fraction. In this analysis‚ their fractional composition‚ saturated vapor pressure‚ density and antiknock rating will be investigated. By knowing the qualitative and quantitative composition of the gasolines‚ the content of n-paraffin‚ isoparaffin‚ aromatic‚ naphthene‚ and olefin (PIANO)
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By: Ella Goldberg Characterization Being different is difficult‚ especially when you’re a teenager. It’s not easy being singled out and feeling like the bad apple. Standing apart from others can be hard and takes courage. Society does not appreciate misfits‚ especially trouble makers who don’t make any effort to succeed. There is nothing wrong with being different and standing up for what you believe in but Holden goes about it in an arrogant way. It’s as if he sets himself apart from his peers
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Elisa’s Characterization in “The Chrysanthemums” In Steinbeck’s “The Chrysanthemums” Elisa Allen is a lonely woman who enjoys growing and nourishing her chrysanthemums in the valley of Salinas‚ California. But‚ with all the beauty of the beautiful valley she feels isolated from the world. “The high grey-flannel fog of winter closed off the Salina Valley from the sky and from all the rest of the world. On every side it sat like a lid on the mountains and made of the great valley a closed pot
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The Separation of Ink Chromatography Emanuel Alvarado Period 06 Group Members: Jason Fernandez‚ Reyna Favela & Lucero Ochoa I. Title: The Separation of Ink Using Chromatography II. Date: October 3rd‚ 2012 III. Purpose: To separate a mixture using paper chromatography. IV. Procedure: 1. Fill beaker with 100 mL water. 2. Poke hole in filter paper with scoopula approximately 1 cm from top. 3. Place dot of ink approximately 2 cm from the bottom of the filter paper
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Micro-extraction of urine and/or stomach contents and analysis by TLC Abstract Thin Layer Chromatography (TLC) is one of the oldest chromatographic techniques used for the identification of compounds and for determination of the presence of trace impurities. The aim of the experiment was to determine two unknown samples (X and Y) by comparing results to the known standard drugs‚ of which there was five‚ using a specific TLC method. The same technique was used for the determination of
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Insertion and amplification of EGFP protein into pET41a(+) Plasmid Introduction The overall purpose of the experiments conducted is to test the creation of recombinant plasmid using recombinant DNA technology. The research of recombinant DNA began with the use of E.Coli (Escherichia coli)‚ a common bacteria found in the intestines of warm-blooded or- ganisms (5). Scientists worked together and generated a way‚ from cloning and using recombi- nant research‚ to achieve recombinant DNA
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Title Identification of Analgesic Compounds Using Thin Layer Chromatography Abstract The objective of laboratory experiment six was to identify an unknown analgesic tablet using Thin Layer Chromatography (TLC) analysis. The procedure involved preparing TLC plates‚ solubilizing standard analgesics (ibuprofen‚ aspirin‚ acetaminophen‚ caffeine) and the unknown in isopropyl alcohol (IPA)‚ and developing the plates in acetone. Rf values were calculated for each compound. The unknown tablet showed an Rf
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PROTEIN ARTICLE RESEARCH SCI/241 PROTEIN ARTICLE RESEARCH Proteins are a part of every cell‚ tissue‚ and organ in our bodies. The protein we eat is broken down by amino acids that are later used to replace proteins in our bodies. These proteins include meats‚ poultry‚ fish‚ eggs‚ nuts‚ seeds‚ milk and milk products. Proteins are made up of amino acids. There are 20 different amino acids that join together to make up all types of protein. A complete protein source is one that provides all
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Protein in the Digestive System Taylor Adams Biol 112- 501 18 April 2016 Introduction Proteins are found in nearly all foods that we eat. Once the food we eat makes its way to our stomachs‚ pepsinogen is released from chief cells. This enzyme mixes with hydrochloric acid in the stomach and begins to break down the proteins. Along with the stomach‚ the small intestine is also an important location for protein breakdown. The proteins from both locations are broken down into amino
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