relationship between absorbance and transmittance. Other learning objectives are to create a Beer’s law plot for a series of samples with known concentrations‚ collect spectrophotomic data from unknown and known FDC blue dye samples‚ perform serial dilutions‚ calculate concentrations‚ perform linear regression and determine the equation of a best fit line. DISCUSSION/OBSERVATION A solution is composed of a solute dissolved into a solvent. The most common solvent is water. Determining the components
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1. EGG INOCULATION The fertile hen’s egg can be used to cultivate and propagate various types of viruses. Because of the ability to alter their tropism and to adapt to a new host species‚ many viruses become capable of growing in chick embryo tissues wherein they frequently attain a much higher concentration than in the tissues of the natural host. STRUCTURE OF AN EGG The extra-embryonic membranes of the chick embryo arise from three germinal layers: the endoderm‚ mesoderm and ectoderm
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glucose and galactose‚ but its function diminishes with age resulting in a wide use of lactase supplements. This enzyme functions under specific conditions‚ so we investigated the effect different salt concentrations have on the enzyme activity. Serial dilutions were performed to prepare a lactase solution as well as twenty percent‚ fifteen percent‚ and five percent concentrations of NaCl. Three different treatments of ortho-nitro-phenyl-galactoside‚ lactase‚ and perspective salt concentrations were
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Water memory Water memory is the claimed ability of water to retain a "memory" of substances previously dissolved in it to arbitrary dilution. No scientific evidence supports this claim. Shaking the water at each stage of a serial dilution is claimed to be necessary for an effect to occur. The concept was proposed by Jacques Benveniste to explain the purported therapeutic powers of homeopathic remedies‚ which are prepared by diluting solutions to such a high degree that not even a single molecule
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Seung Soo (Jason) Lee 002213-065 Internal Assessment – Investigating the Relationship between Concentration of Sodium Chloride and the Rate of Reaction of Enzyme Amylase Research Question: How will changing the percentage of sodium chloride concentration affect the rate of reaction of enzyme amylase‚ measured using the absorbance of starch and iodine with a spectrophotometer. Introduction: Amylase is an enzyme that is involved in the human digestive process. Found in both the human pancreas
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concentration of the coloured complex can be determined with the spectrophotometer at Absorbance 540.The sugar concentration of unknown sample can then be read off a calibration curve(standard curve) created using known sugar concentrations.The dilutions of a solution of known concentration are used to determine the concentration of unknown. Being familiar with the background information about reducing sugars and various methods used to identify them‚Biotechnology students were provided with a
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best because a solid medium that provides a surface for the individual cells to be separated and isolated from the other microbial cells that may be present in the original sample. Also the pour is more likely to gain unwanted bacteria through the dilution process. D. What are the six qualities included in a description of colony morphology? 1. Shape: What is the basic formation of the colony? Is it circular‚ irregular‚ or
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Microbiology Lab Quiz #2 Study Guide Exercise 9 -- The Gram Stain Compare and contrast simple and differential staining techniques. Simple Staining Simple staining is useful in determining the basic morphology of an organism. Simple staining involves only one reagent i.e. crystal violet‚ basic fuchsin‚ or methylene blue. Simple dyes used to stain bacteria have a positive charge cationic (+)‚ therefore‚ they are attracted to the bacteria that are usually negatively charged anionic (-) Depends
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Introduction: Enzymes are biological catalysts that permit cells to carry out the many functions that are required in a living system. Every enzyme has a specific substrate and a specific function. Enzymes alter substrates one of three ways: by adding something to the substrate‚ removing something from the substrate‚ or by changing its conformation‚ otherwise known as its shape. The structure of an enzyme and its ability to function exists because it binds to an active site‚ thus increasing the
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Blood Cell which is an indicator of the presence of virus. ■ Magnitude of the agglutination depends on quantity of virus. ■ Two fold serial dilution of the suspected virus material in P.B.S is treated with 1% washed R.B.C.s. ■ Virus agglutination can be seen in micro titration plates. ■ Reciprocal of the last virus dilution giving agglutination is Haemagglutination titer (HA titer). [pic] HAEMAGGLUTINATION INHIBITION TEST ■ Haemagglutination activity of the
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