Buffer Preparation (Gozani Lab) 1. 1 M Tris-HCl Buffers pH Volume (L) TrisBase (g) HCl (ml) pH 7.0 2 242.2 150-155 pH 7.5 2 242.2 120-125 pH 8.0 2 242.2 80-85 Autoclavable. 2. EDTA 0.5 M (pH8.0) 0.5M‚ 1L: 148 g EDTA + ~30-40 g NaOH to adjust pH (or 186 g EDTA-Na.2H2O + ~20 g NaOH) Note: pH adjusted by NaOH is essential for solubility. Autoclavable. 3. TAE DNA Electrophoresis Buffer (50 X) (2 M Tris‚ 50 mM EDTA) 2L 484 g Tris 114.2 ml glacial acetic acid 200 ml 0.5 M EDTA 8.0 To make
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Penicillium chrysogenum ATCC 16520 (Obtained from American Type Culture Collection) is used for the production of Penicillin. Preparation of Different Salts of Phenyl acetic acid. Sodium phenyl acetate For 100 ml preparation‚ few grams of PAA (of known percentage) are dissolved in certain volume of De-Mineralized water. The pH of the solution is maintained between 6.6-7 by adding NaOH in drops. Molecular Weight-159. Potassium phenyl acetate For 100 ml preparation‚ few grams of PAA (of known percentage)
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Examination preparation Being student os college is the dream of so many people‚ only way to be succeed. It is not hard to be college student but it is not easy to get through and graduate from college. There are so many class to take and also pass each examination of each class. Each student has their own way to study and prepare for their examination. The best way to have good preparation of examination is just only two steps.Timing and focusing is 2 major steps that we should know and follow
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discarded the lower aqueous layer. 6. Added approx.15 ml of 1mol/L Na2CO3 solution.Shaked‚drained and discarded the lower layer leaving the ester in the separating funnel. Diagram : Observations : From our results‚the ester we made produced a light banana smell. In this experiment for preparation of an ester as the alcohol used was pentanol and the acid used was glacial acetic acid‚ added conc.sulfuric acid a dehydrating agent used to remove the moisture‚ safety was a priority in this experiment
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EXPERIMENT A PREPARATION OF ASPIRIN Abstract This report gives a detailed account of the experimental preparation of aspirin in a laboratory environment as well as some basic industrial background on the product. It also contains information about safety precautions put in place to ensure the safety of the team who carried out the experiment. All results obtained have been included as well as a detailed analysis of what they represent alongside any improvements to the method used. Introduction
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have been selected from a vast number of candidates based of topics related to your experience and education， so you may need to bring more to the interview. Reviewing your resume in an interview will only result in boring the interviewer‚ inadvertently lowering your chances to become the succeeding candidate. Preparation is the key to success in approaching the opportunity of the job interview. According to article 1‚ there have six key steps in job interview preparation. The first step is know
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Interview Project Report Your name: Your partner’s name: PART 1. IntervieweE report Name of the company: Ritz Carlton Note: The company must be the organization you chose for your Media Case Project. Interview preparation Complete the following: Your spokesperson function in the company. Explain why this spokesperson is best suited to handle this specific interview‚ based on the information provided by the journalist. The spokesperson is the CEO because he is the best person to
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20 – 03 – 13 EXPERIMENT: - PREPARATION OF POLYURETHANES AIM Synthesis of polyurethanes OBJECTIVES - Mixing a diisocyanate and a diol in polyurethane synthesis of two distinct products. - Comparing polyurethane from castor oil to that made from polyethylene glycol. - To establish the type of foams in terms rigidity or flexibility. THEORY Polyurethanes‚ also known as polycarbamates‚ belong to a larger class of compounds called polymers. Polymers are macromolecules made up
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Preparation of Bromotriphenylmethane Abstract The structure of a major component of star anise oil was derived by analyzing its IR spectroscopy melting point of after oxidation and purification. From the evidence obtained‚ it was concluded that the exact structured should be p-methoxybenzoic acid. Introduction The purpose of this experiment is to determine the structure of the major component of star anise oil. It is already known that this component has a molecular formula C10H12O. This
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program‚ and a baccalaureate degree program in which graduates of all three programs have to take the National Council Licensing Examination for RNs (NCLEX-RN©) which measures competency for entry-level nursing practice (Raines & Taglaireni‚ 2008). From these entry-level positions‚ there are even more options to further nursing education‚ such as RN-BSN degree-completion program‚ master degree programs‚ and BSN-doctoral programs (Raines & Taglaireni‚ 2008). In the United States‚ approximately 60%
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