Title of Lab: Preparation and Assay of Phenolase and Peroxidase from Sweet and Irish Potato Hypothesis: Polyphenol Oxidase enzyme activity can be detected by change in colour of solution‚ Inhibitors prevent the reaction of the enzymes with substrates‚ the enzyme is relatively specific. Aim: To design and conduct an experiment to demonstrate enzyme activity of Polyphenol Oxidase and Peroxidase when mixed with catechol‚ caffeic acid‚ pyrogallad‚ tyrosine‚ guacol‚ and water‚ to test the effect of
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| An acid is a chemical substance any of various typically water soluble and sour compounds that are capable of reacting with a base to for a salt‚ that redden litmus paper‚ that evolve hydrogen on reaction with various metals‚ that in water solution yield hydrogen ions‚ and that have hydrogen containing molecules or ions able to give up a proton to a base or that are substances able to accept and unshared pair of electrons from a base. An alkaline is a chemical compound that neutralizes or effervesces
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Assignment Preparation: Activities include watching the Lynda.com video‚ independent student reading‚ and research. Watch the Lynda.com video‚ "Insights on Software Quality Engineering." Think of a software application (for example‚ a time tracking application) that you would like to put in for your past or present organization‚ or an organization that you have read about. Use the table below to describe as clearly as you can‚ the specific artifacts in each phase‚ and an example of how the artifact
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involvement of local level representative institutions in the formulation of plans for development as well as their implementation is being advocated in the interest of efficient utilisation of resources and for ensuring more equitable sharing of benefit from development. Decentralisation of the planning process is basically an exercise in multi-level planning. Although multi-level planning and the problems connected with it have only recently been subjected to serious examination in India‚ the idea
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Experiment 2 Title: preparation of a halogenoalkane Objective: To synthesis 2-cholo-2-methylpropane by using corresponding alcohol and HCl(aq). The reaction is via SN1 of tertiary alcohol. Techniques: i) Distillation of the reactant and the product ii) Purification by separating funnel Theory: In the experiment‚ SN1 reaction take place‚ HCl is added to initiate the reaction. The R-OH attack the H+ with the lone pair on O. Then the R-O+H2 bond breaks the release a water molecule and
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CHOONG SHING YI CHOONG SHING YI Preparation and Recrystallization of Aspirin Preparation and Recrystallization of Aspirin Date of the experiment: 9 May 2013 Date of submission: 23 May 2012 Date of the experiment: 9 May 2013 Date of submission: 23 May 2012 Synopsis 1. General Objective: To familiarize with the preparation of some simple organic compound and purify the compound by recrystallization. Specific Objective: The experiment is carried
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Standardization of NaOH and Determination of Unknown Acid KEYWORDS: quantitative analysis‚ titration‚ buret‚ endpoint‚ standardization‚ half-equivalence point‚ calorimetric titration‚ potentiometric titration ABSTRACT: The concentration of sodium hydroxide was determined by colorimetric titration‚ and the identity of an unknown acid was determined by potentiometric titration. In the first titration‚ a strong acid standard‚ potassium hydrogen phthalate (KHP)‚ was used‚ to determine the concentration
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perspective of nursing‚ spiritual perspectives‚ nurse to human diversity‚ ethical‚ legal‚ social influences and historical concepts of nursing. According to the University of Texas at Arlington “baccalaureate graduates are prepared to synthesize information from various disciplines‚ think logically‚ analyze critically‚ and communicate effectively with clients and other health care professionals” (College of Nursing‚ 2010). They go on to say that “graduates are expected to demonstrate all the competencies
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The Preparation of 1- Bromobutane Introduction... Aim : To prepare 1-Bromobutane. Background : The most common way of preparing alkyl halides‚ which are very useful intermediates in syntheses‚ is the replacement of the OH group of an alcohol by a halogen. This replacement is a nucleophilic substitution reaction‚ and alcohols do not undergo nucleophilic substitution reactions because hydroxide ison is strongly basic and a poor leaving group. However‚ alcohols readily undergo
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Preparation of 4-bromoaniline Introduction Aromatic compounds tend to undergo electrophilic aromatic substitutions rather than addition reactions. Substitution of a new group for a hydrogen atom takes place via a resonance-stabilized carbocation. As the benzene ring is quite electron-rich‚ it almost always behaves as a nucleophile in a reaction which means the substitution on benzene occurs by the addition of an electrophile. Substituted benzenes tend to react at predictable positions. Alkyl groups
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