Nov 19/12 Solute Concentration of Potatoes Block 2-2 Purpose: To determine the concentration of solute in the potato’s cytoplasm by measuring the change in mass after the process of osmosis. Materials and Equipment: Refer to Biology 12 Lab Manual – Investigation 13 Procedure: Refer to Biology 12 Lab Manual – Investigation 13 Data and Observations: The Potatoes change in mass after the process of Osmosis Test Tube # | Concentration
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was added to the funnel and shaken to mix the layers. The lower organic phase was drained into the Erlenmeyer flask and the aqueous phase out of the funnel. After that‚ the organic layer was returned to the separating funnel and washed with 15% NaCl solution. Anhydrous sodium sulfate was used as the drying agent and allowed the solution to stay for 15 minutes. The Erlenmeyer flask was weighed and the pipet was used to transfer the solution from the drying agent to the flask. Finally‚ the hood
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values (10‚ 7‚ 6‚ and 3) Change in color of the solution The amount of potato extract‚ pH solution‚ and catechol used (1 cm +/- .1cm) Size of the test tubes Amount of time allowed for the catechol to sit with the potato extract and pH solution (20 minutes with 5 minute intervals for observation) Materials: 4 test tubes Test tube rack Wax pencil Small ruler Buffer solutions of pH 10‚ 7‚ 6‚ and 3 Potato extract Catechol Procedure: 1. Using the small ruler and the wax pencil‚ three
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Abstract: Enzymes‚ molecules that speed up chemical reactions‚ are specific to one substrate. In this experiment the substrate hydrogen peroxide and the enzyme catalase will be used. The higher the concentration of potato extract‚ or catalase‚ the faster the reaction and the more substrate present will result in a decrease in the time of the reaction. The amount of concentrations of enzymes and substrates are changed to determine if the reaction is further catalyzed by a greater concentration of
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Exercise 1C 1.2 GRAPH 1) Molar concentration of sucrose= percent change in mass Exercise 1D 1) If a potato core is allowed to dehydrate by sitting in the open air‚ would the water potential of the potato cells decrease or increase? As it dehydrates‚ the solutes become more concentrated so the solute potential becomes more negative and it decreases. 2) If a plant cell has a lower water potential than
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RECOMMENDATION REFERENCES RESEARCH PLAN Materials and Methods To make a good quality of cream‚ the necessary materials needed in making cream will be gathered by the researchers. The research will collect 375 ml of kamote leaves extract‚ 600 ml of vegetable oil‚ 175 grams of paraffin wax. Research Design The research will employ the Static-Group Comparison Design. Static Group Comparison Design – two already existing‚ or intact‚ groups will be used. These are sometimes referred
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tubes 2 small beakers A dozen filter paper disks Test tube rack Hydrogen peroxide (H2O2) Potato extract Forceps Thermometer Hot plate Large beaker Ice cubes Graduated cylinder Stopwatch Procedure: Step 1 Place 10 mL of potato extract in a small beaker and check the temperature to ensure that it is at room temperature (20-25°C). Step 2 Using forceps dip a filter paper disk into the extract and drop it into the test tube with 10mL of hydrogen peroxide. Step 3 Allow the filter paper
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specific pH level would impact tyrosinase activity. First we added 4.0 mL of pH in each corresponding test tube and then added 0.5 mL of substrate (catechol) into each test tube. In the instructions it says to apply your 0.5 mL of tyrosinase (potato extract) as well but you have to blank the spectrophotometer before. The results from this experiment confirms that our hypothesis of a neutral pH displaying a stronger impact on tyrosinase is true. According to our results the pH levels at a more neutral
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We were to indicate measurements on each test tube at 1cm and 2cm from the bottom. We then identified each test tube as 2a‚ 2b‚ and 2c. - We filled each test tube with the following: 2a with 1cm of potato extract containing Catechol Oxidase and 1cm of 1% Catechol Solution; 2b with 1cm of potato extract containing Catechol Oxidase and 1cm of dH2O; 2c with 1cm of 1% Catechol Solution and 1cm of dH2O. We tapped each tube to completely mix the solution and recorded the time at zero. - At zero time‚ each
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The effect of Sodium Chloride (NaCl) salt concentration on osmosis in potato cells The movement of substances in plant cells involves many processes and systems‚ all of which may affect the plant bio-chemically and physically‚ and one of these processes is osmosis. Osmosis is the flow of water through a semi-permeable membrane of a cell moving from an area of higher water potential to an area of lower water potential until reaching equilibrium known as isotonic. Before reaching the point of being
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