= 19.667 mL The molarity of NaCl solution = MNaCl = MAgNO3VAgNO3/VNaCl = = 0.0492 M The calculated concentration of NaCl = MW of NaCl x Molarity of NaCl = 58.44 g/mol x 0.0492 mol/L = 2.87ถ g/L Table 2: Determination of salt in butter - Titration Titration Titre Volume (mL) 1 6.3 2 6.2 3 6.2 The average titre value = = 6.233 mL The molarity of NaCl extract solution = MNaCl = MAgNO3VAgNO3/VNaCl = = 0.0125 M The amount of NaCl in 100mL extract = 0.0125mol/L x L x 58.44g/mol
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Stacy Hernandez Period 1 Mrs. Riley AP Biology Osmosis and Diffusion Lab I. Introduction: Diffusion is vital to many life functions of a cell‚ it allow the transportation of vitally important nutrients and compounds without the expenditure of excess metabolic energy. To explain diffusion‚ it is as if a bottle of perfume is opened at one end of the room‚ then in a short amount of time a person at the other end of the room can detect the scent of the perfume; this is the process of diffusion
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A STUDY OF OSMOSIS Han Cheng Water passes through aquaporins in cell membranes from an area of high water concentration (low solute concentration) to an area of low water concentration (high solute concentration). This process is called osmosis. It requires no cellular energy to be used‚ and occurs due to the random‚ continuous motion of all molecules. If a cell is placed in an environment in which the concentration of water is less than in the cell (hypertonic)‚ water will flow from the cytoplasm
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in which a potato catalase enzyme reacts to various assays with differing variables. To do so a baseline assay (undiluted extract and room temperature H2O2) was used within the experiment with only one other variable changed in the other assays. These variables included a boiled‚ frozen and then thawed‚ and frozen potato extract and dH2O instead of the potato extract. It was noted that the temperature and or way the potato extract was prepared effects how the enzyme with the potato will react
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experiment was broken into three sections. The first section showed the pH and color changes that an enzyme can create. It was predicted that when potato extract‚ the enzyme‚ was added to catechol‚ the substrate‚ an enzymatic reaction would occur. The second section of the experiment demonstrated enzyme specificity. It was predicted that potato extract‚ when hydroquinone was introduced‚ would not exhibit the characteristics of an enzymatic reaction. The third section of part one focused on whether
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Therefore‚ it will be presumed that the result of this experiment by isolating the polyphenol oxidase within the potato extract through the oxidation of 5mM dopa solution with highly concentrated enzymatic preparation of polyphenol oxidase with a buffer as the diluent will indicate a rapid increase in the rate of the reaction as well as the amount of activity present in the potato extract. Proposing that Vmax is proportional to enzyme concentration‚ a linear relationship should be present and enzymatic
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Pre-lab: look up the structure of lycopene. Introduction: Lycopene is the red pigment in ripe tomatoes and‚ as an antioxidant‚ helps to fight certain cancers. In this lab you will isolate lycopene from tomato paste. To do this you will first extract carotenoid pigments from the paste and then use column chromatography to isolate the lycopene from the other pigments. You will then use TLC to evaluate the column chromatography separation. Note: because lycopene is light-sensitive‚ prevent any
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pH and Temperature on Enzyme Activity” Abstract: In the following experiments we will measure precise amounts of potato extract as well as Phenylthiourea‚ combined with or without deionized water and in some instances change the temperature and observe and record the reaction. We will also investigate the different levels of prepared pH on varying samples of the potato extract and the Phenylthiourea and record the results. We will answer question such as what is the best temperature for optimum
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separated in this lab contains three components: naphthalene‚ C10H8‚ common table salt‚ NaCl‚ and sand‚ SiO2. The separation will be done according to the scheme shown on the next page and involves three basic steps: 1. Heating the mixture to sublime the naphthalene. 2. Dissolving the table salt with water to extract. 3. Evaporating water to recover dry NaCl and sand.2 Mixture: naphthalene‚ NaCl heat to naphthalene
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a sodium chloride (NaCl) solution‚ subjecting the product to detergent solution (dH2O)‚ filtering the solution and lastly‚ the addition of ethanol. When beginning with a solid substance‚ such as a banana‚ crushing the substance allows for breaking down the cell membrane and cell wall‚ if using a plant cell. This ultimately‚ removes the contents of the cell from the deoxyribonucleic acid (DNA) and further exposes it for the detergent solution. After this step‚ one would use NaCl solution to stabilize
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