Lab report nº4 The aim of this experiment was to observe the change of enzyme reaction with different concentration of solution. For this experiment we used potato enzymes (catalase) and hydrogen peroxide in concentrations of 100%‚ 80%‚ 60%‚ 40%‚ and 20% According to P.George: “When catalase is added to hydrogen peroxide‚ there is an initial rapid evolution of oxygen which lasts for about two minutes‚ depending on the peroxide concentration. After this‚ oxygen is given off at a steady rate which
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Title: Potato Osmosis Abstract: In this experiment was designed to study the effect of a concentrated solution applied to white and sweet potatoes. The solution’s impact among the potatoes were to be either hypertonic‚ hypotonic‚ or isotonic. The potatoes were first cut into fries with a fry cutter‚ and then weighed after being cut. The white and sweet potatoes’ weights ranged from .005-.015 grams. A total of 18 sugar solutions are to be made over the course of 3 trials. In each trial‚ 6 different
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The Effect of Temperature on Enzyme Activity and Oxygen Production Throughout this report you will gain information as to how temperature effects the amount of oxygen produced in an enzyme- catalase experiment. In the experiment we used liver extract as a catalase and created a chemical reaction within a reaction chamber between the catalase and hydrogen peroxide as well as three different controlled temperatures. In the procedure below there will be a step by step process as to how
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Erin Arroyo Lab report June 11‚ 2013 Biology 123 Professor K Title: Scientific Investigation of the Peroxidase Enzyme & Temperature Abstract: In this lab we tested the effect temperature has on the rate of enzyme activity. The way we figured this out was by taking four different temperatures and testing the different absorbance levels they produced every 20 seconds for two minutes straight using a spectrophotometer. The important part of this experiment was the temperature
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experiment‚ you will study osmosis. Your task is to design an experiment that will determine what concentration of solute is isotonic with the specimen cells. (Design) Procedure: You will be provided with the following materials: Potato‚ potato corer‚ scalpel‚ forceps‚ electronic balance 10 % salt solution‚ distilled water‚ 50 mL beakers‚ graduated cylinder‚ paper towel You must design a procedure that will enable you to determine the concentration of salt that is isotonic
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Abstract The experiment was carried out to investigate at which point the potato has reached its isotonic environment. From the results‚ when the concentration increase to 0.3M‚ the mass of the potato slices decreased by -3.26%. We can conclude we see that as we increased the sucrose concentration it was a decrease in the percent change. Introduction All cells have membranes that are selectively permeable. In other words‚ they allow certain things in and certain substances are not allowed to
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Stallings Period 2 January 26‚ 2013 Enzyme Catalysis Lab Report Background: Enzymes are catalyst‚ which affect the rate of a chemical reaction. One consequence includes the cell to carry out complex chemical activities at relatively low temperatures. In these reactions the substrate binds reversibly to the active site. The cause of this is a decrease in the energy needed to activate the reaction of the substrate molecule to from products. Every enzyme is particular for a reaction for the reason
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cell‚ so the water molecules diffused out of the cell through the selectively permeable membrane. The higher the concentration‚ the more plasmolysed the cell becomes and my results show this. The 0M bathing solutions in both experiments caused the potato cells to become slightly turgid‚ this means that there was a higher concentration of solution on the inside of the cell. So the water molecules diffused into the cell through the selectively permeable membrane. The rate of water loss decreases as
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Chemistry 512 Enzyme Catalysis Lab Report Pre-lab Questions: 1. Write a balanced chemical equation with state symbols for the reaction catalyzed by peroxidase. 2H2O2 2H2O + O2 (4H1 4O) (4H + 2O + 2O) 2. What is the substrate(s) of this reaction? What is the catalyst? Substrate = H2O2 hydrogen peroxide Catalyst = peroxide 3. At what approximate temperature do enzymes normally operate in the body of a warm-blooded animal? Would your answer change if the enzyme came from a plant or yeast? Enzymes normally
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Effect of Temperature on Enzymes ~Abstract~ In this experiment the effect of different types of temperatures on enzyme activity was examined. The temperature baths that were used to test the difference in enzyme activity on fresh liver were; 4 °C‚ room temperature which was 22°C‚ body temperature which is 37°C‚ and 77°C. The total time of each trial was 2 and a half minute‚ 1 minute for the H2O2 to acclimatize to the temperature‚ 1 and a half minutes for the reaction to occur. Catalase causes Hydrogen
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