and centrifuged for 20 seconds. A polyacrylamide gel was assembled and the samples were loaded into it. The gel ran for approximately 45 minutes at 160 V. A western blot analysis was done in order to examine the Erk1/2 phosphorylation of the NIH3T3 cells. Three pieces of Whatman paper were wet in transfer buffer and placed on the anode plate. A wet PVDF membrane was placed on top of the Whatman paper. The gel was removed from holder and the stacking gel was removed. Forceps were used to place the gel
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Electrochemical Cells Lab Report AP Chemistry Block 1 Analysis: The purpose of Part 1 of this laboratory is to construct a table listing the reduction potentials of a series of metal ions in order of ease of reduction. The series of half-cells is constructed by placing a piece of metal into a 1.0 M solution of its ions for each metal in the series. The metals are Cu‚ Fe‚ Pb‚ Mg‚ Ag‚ and Zn. The half-cells are connected by a salt bridge constructed of a strip of filter paper soaked in a solution
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TITLE AND AUTHOR Lab 7 Analysis of purified Concanavalin A via:Hemagglutination INTRODUCTION The purpose of this lab was to test the biological activity of ConA by performing a hemagglutination assay. If ConA is active then agglutination will occur due to ConA’s free receptors being able to bind to the glucose residues on the sheep’s red blood cells. If ConA is not active then no agglutination will occur. To test the hemagglutination reaction‚ two types of ConA solutions
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Testing Cell Transportation Across a Membrane Introduction Cells have the amazing ability to transport certain molecules in or out of their membrane. Some require no energy to do so (passive transport) while others require energy to be processed through (active transport). There is also the transportation of water across a membrane‚ which has its own term of osmosis. Too much of something can be taken in‚ or too little enters. This especially happens to plants‚ who require water (and sun) to live
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Lab Report Purpose: Osmosis and diffusion through potato core in different concentration (viable) : The concentration of solution in a solution will affect the movement of water across potato cell membrane Material: Potato core Sucrose solution (0.2‚0.4‚0.6‚0.8‚1.0) Electronic Balane Test tube and rack Scalpel Plastic Weighing tray Cork Borers Method: Day 1 use you cork borers to bore 6 vertical hole on your potato. remove the potato cylinders from
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Potato Catalysis Lab Pre-Lab Assignment Background Information: An enzyme is catalytic protein. It is the most important type of molecule found in living cells. Cells would not be able to function without enzymes. Enzymes speed up or slow down chemical reactions of the cells. It is usually easy to identify the names of enzymes because they end in -ase. The enzyme that acts upon the substrate hydrogen peroxide is usually called catalase. This enzyme is found in both plants and animals
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concentration gradient until each side of the membrane are equal. The water moves because the membrane is impermeable to the solute and the solute concentrations may differ on either side of the membrane. Water molecules may move in and out of the cell‚ but there is no net diffusion of water. Water will move in one direction or the other‚ and this is determined by the solute or solvents concentration levels. If the two solutions are of equal concentrations‚ they will be isotonic. If the concentrations
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because the high amount of salt causes more dehydration. Variables: Independent- Salt Solution Dependent- Mass of Potato Control- Tap Water Materials: •Tap Water (50 ml for each cup) •Salt •Beaker •3 cups •3 pieces of potato •Scale Procedure: Step 1: Gather Materials. Step 2: Weigh each potato separately on the scale and record weight in grams on table. Step 3: Put each potato in a separate cup and add 50 ml of water to each cup‚ measure with beaker. Step 4: To create the 40% salt solution
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BioLab3 Lab Report 7 Cellular Respiration Answer Key Student Name: I. The ATP Cycle Define the following terms: Autotroph Heterotroph Aerobic respiration Anaerobic respiration Complete the chart below: Letter Defining Term A B C D II. Anaerobic Respiration Define the following terms: Alcoholic fermentation EXERCISE 1 – Alcoholic fermentation At intervals of 20‚ 40‚ and 60 minutes‚ the tubes are removed. Record the
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Microscopy‚ Cell Structure and Function Task 2 I: Name and describe the four main types of tissues in animals stating their functions and their location in the body give two examples of each type. The four main types of tissues that you will find in eukaryotes are connective‚ muscle‚ nervous and epithelial tissues; each of which having very different roles. Connective Tissue The first tissue type I am going to look at is the connective tissue. This tissue is found all over the body‚ including
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