LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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was cut with restriction enzyme Sal I to prepare both for ligation. 3. Vibrio fischeri and pGEM DNA were ligated with T4 ligase. 4. E.coli DH5α competent cells were prepared for us so that transformation of the recombinant plasmids and the competent cells through heat shock transformation. 5. The transformation was then plated on LB/amp/X-gal plates. 6. The plates were screened for our target with ampicillin resistance‚ blue/white colony‚ then bioluminescence. 7. A bioluminescent was selected and
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now. I saw the fear in Bob’s eyes as the blade sliced through the air‚ brushing over his throat‚ making a clean‚ straight line. Time seemed to slow‚ each heartbeat reverberating in my ears as the world narrowed down to just me and him. My love for Ponyboy consumed me‚ and in that moment‚ I knew I had to protect him at any cost‚ even if it meant ending Bob’s life. He was a bloody brother and nothing could ever break that bond. The impact was jarring‚ a sickening
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success? Refer to relevant case studies and example in your discussion. Business operations involve the processes of transformation or more generally‚ production. This involves the transformation of business inputs and business out puts. The business operations may be influenced by the quality expectations‚ technology and globalisation. Business may use the inputs‚ transformation processes and outputs to help them build their business to success. A quality expectation is one of the key goals of
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10.1 HSC topic: Operations -> is about planning & controlling operations with the aim of minimizing cost‚ maximizing productivity‚ improving efficiency and achieving strategic business goals 25% of indicative time The focus of this topic is the strategies for effective operations management in large businesses. Outcomes The student: H1 critically analyses the role of business in Australia and globally H2 evaluates management strategies in response to changes in internal and external
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2/15/2013 background on transformation of bacteria with pGLO plasmid Experiment #5 Aim: Purpose of this lab is to have plasmid activity transformed Material: Bacteria starter plate‚ pGLO DNA Plasmid‚ microcentrifuge tubes‚ Ice‚ water bath‚ CaCl2 Transformation solution‚ (LB) agar plate‚ (LB/Amp) agar plate‚ (LB/Amp/ara) agar plate‚ Micropipette‚ and Micropipette tips. Method: Genetic transformation is a procedure which is done by taking genes from one organism and putting them in another organism
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Ponyboy Curtis what are the physical traits of ponyboy curtis in the book? (long hair‚ light brown hair). Ponyboy is 14 year s old and he has light brown (almost red) hair thats greased and combed back‚ he has greengray eyes. He is considered small and slim. He is a daydreamer‚ a bookworm‚ and a heavy smoker. He analyzes many things deeply. He is very smart and has good literary capability. Ponyboy is also a little naive and is not overtly connected to reality. He matures throughout the course of the novel
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the world and made the world electricity dependent when compared to the people in the past. ARGUMENT 1: When we observe our present scenario‚ we can see that technology has been the dominant area of knowledge which has lead to the rapid transformation of the world. MAIN EXAMPLE: In the 1900’s people travelled a lot and waited for long hours to buy products and book tickets. They used to move from library to library to gather information for a topic but at present
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Thank you for meeting with me today‚ Ponyboy. You were once one of Tulsa’s “juvenile delinquents”‚ yet you became a hometown hero by saving the kids at the church. How has that affected your life? Well‚ it’s true that I once was a hood‚ but now I have a family and my brothers also and I am a author. What is your earliest memory of your friends? When we were hanging out when Johnny and Dally were still alive. How has the gang changed since Johnny’s death? We were sad but got use to it‚ we will miss
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An organization creates value by converting inputs into outputs. It takes several resources such as raw materials‚ machinery‚ information‚ knowledge‚ human resources‚ money and capital as inputs and applies appropriate production and transformation processes at the conversion stage to produce finished goods and services. Consumers purchase these goods and services to satisfy their needs. The environment‚ in which an organization operates‚ affects stages in the value-creation process
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