E. LOOK AT JOURNAL ARTICLES FOR AN EXAMPLE) ALL ASSAYS MUST BE INCLUDED TO RECEIVE FULL CREDIT (Growth curve‚ CFUs (counting colonies/dilutions‚ can include equation)‚ Growth after incubating at RT for 6 days‚ MIC (antimicrobial disc)‚ Catalase‚ Oxidase‚ DNA isolation). Under each headline describe the methods and material. The example below includes for headline that should be included but not all. ALL ASSAYS MUST BE INCLUDED TO RECEIVE FULL CREDIT and MAKE SURE YOU READ YOUR PROTOCOLS I GAVE TO
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Principles of Biology Lab Exercise Enzymes: Catalysts of Life Instructor: Professor Alcendor By Shahid Rana Date: March 7th‚ 2013 Abstract: In this experiment we have demonstrated the function of enzymes. The whole experiment was devoted to understand how enzymes work as a catalysts and increase the chemical reaction without being used themselves. In general‚ enzymes are proteins that function as biological catalysts. These enzymes adhere to lower to amount of energy required for
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unknown: 1 Colony Appearance 2 Motility 3 Gram Stain 4 Thyoglycollate aerobic vs. anaerobic 5 Innoculate T soy slant 6 Optimum growth temperature 37o vs. 42o 7 Nitrate test 8 Methyl Red 9 Carbohydrate fermentation 10 Catalase Test 11 Oxidase test
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and Results (Experimental) 1 Milkish white/ milkish white Control To control for color change of the mitochondrial enzymes alone without addition of NADI. (false +) 2 Milkish white/ Bright pink to bluish purple Experiment Assay for Cytochrome oxidase activity. (+ purple) Shows the effects from the addition of NADI‚ a very
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Lab 6.C Hypothesis: If enzyme activity is affected by the pH of a solution‚ then the enzymes will experience the greatest activity at a pH of 6. Variables: Independent Variables Dependent Variable Controls Four different pH values (10‚ 7‚ 6‚ and 3) Change in color of the solution The amount of potato extract‚ pH solution‚ and catechol used (1 cm +/- .1cm) Size of the test tubes Amount of time allowed for the catechol to sit with the potato extract and pH solution (20 minutes with 5
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Next‚ an oxidase test was performed to eliminate one or more of the bacterium choices. P. aeruginosa was eliminated as the identity of the bacterium when the oxidase test results were negative. An EMB plate of the bacteria was examined to identify whether the bacterium was S. typhi or E. coli. The growth on the EMB plate was shiny and metallic
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5cm‚ 6cm‚ at the bottom of each test tube. 3. Put your test tubes to the location of the phosphate buffer series and fill each tube according to the following direction. 4. Continue your work area and add 1cm of potato extract obtaining catechol oxidase to each of the seven tubes. 5. Add 1% catechol to each of the 7 test tubes‚ bringing the total volume to the 6c mark. Agitate the contents of the tubes using a vortex mixer if available. 6. At time 0‚ record the relative color intensity of each
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Name: Madison Gullatt Sect/Table: !/D Experiment 8 Report: Extracellular Enzymes Purpose: The purpose of this experiment is to perform a variety of different test and record the data from each test in relation to extracellular enzymes. Results: 1. Fill in the following tables with your observations. Note if the test is positive or negative for the enzyme. Also note the appearance of the colonies and/or media. This will include: the color of the medium immediately surrounding
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The purpose of the unknown bacteria lab assignment was to select an unknown bacteria culture and‚ through a series of metabolic tests‚ identify which bacteria genus resided in the pure culture received. A nutrient broth inoculated with bacterial culture (numbered 45‚ henceforth referenced as U45) was selected and a streak plate was made to isolate a pure culture for use throughout the assignment. From the streak plate‚ several slides were made to determine the morphology of unknown 45. A Gram stain
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Present prospective: Latest literature in the area of natural pigments is divided based on their interest to five main potential topics: 1. The first topic is dealing with the chemical composition and the factors influencing that composition. 2. Second topic is concerned with increasing the yield of the pigment by searching the existing plant and microbial sources in addition to alternative microbial and plant sources. 3. The third topic is dealing with improving the stability and bioavailability
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