“CLONING OF HORSE RADISHPEROXIDASE GENE IN E.coli FOR EXTRACELLULAR EXPRESSION” Project report submitted to Jawaharlal Nehru Technological University Hyderabad in partial fulfillment for the award of degree of Bachelor of Technology in Biotechnology Submitted by M.RAVI TEJA (08311A2338) & V.MAYURI (08311A2358) Under the Guidance of DR.CHAND PASHA Department of Microbiology Osmania University [pic] Department Of Biotechnology SREENIDHI INSTITUTE OF SCIENCE AND TECHNOLOGY Yamnampet
Premium Enzyme Molecular biology Oxygen
Biochemistry Eukaryotic Cells (animal cells) * Nucleus * Organelle Mitochondria: “power house” Where energy (ATP) produced and stored Prokaryotic Cells (plant cells) * Cell wall * Chlorophyll * Cytoplasts: where photosynthesis occurs General Equations:- Photosynthesis: * 6CO2(g) + 6H20(l) +E(solar) -> C6H1206(glucose) + 602(g) Respiration: * C6H1206 + 602(g) -> 6CO2(g) + 6H20(l) + E Metabolism: * Thyroxin: A hormone that controls the bodies
Premium Protein Amino acid Enzyme
carbonyl functional groups share these two properties‚ within the carbonyl family of compounds‚ there are significant differences in reactivity. Aldehydes are generally more reactive than ketones and more easily oxidized. Carbonyls directly bonded to aromatic groups are generally less relative than Carbonyls that are directly bonded to alkyl substituents. In this experiment‚ we will perform each reaction on the known compounds that correspond to each chemical test first‚ to determine whether the
Premium Aldehyde Alcohol Carbonyl
ENERGIZER’ help us to Reduce Petrol /Diesel /Cooking gas consumption up to 28%‚ or in other words this would equal to buying the fuel up to 28% cheaper prices. When fuel flow through powerful magnetic field created by Magnetizer Fuel Energizer‚ The hydrocarbons change their orientation and molecules in them change their configuration. Result: Molecules get realigned‚ and actively into locked with oxygen during combustion to produce a near complete burning of fuel in combustion chamber. Mechanical engineering
Premium Internal combustion engine Hydrogen Gasoline
EXECUTIVE SUMMARY One of the most important tasks that a petroleum engineer has to undertake is determining how much hydrocarbons are contained in a potential reservoir. Key physical characteristics that they will be concerned with include porosity‚ permeability‚ net-to-gross ratio and bulk rock volume. This information can then be used to calculate original oil in place (OOIP) and original gas in place (OGIP). Hence‚ for a given exploration prospect‚ explorers and commercial analysts can
Premium Volume Petroleum Map
Methyl Benzoate Objectives: -To prepare methyl-3-nitrobenzoate from nitration of methyl benzoate by electropilic aromatic substitution. -To calculate the percentage yield and get the melting point of methyl-3-nitrobenzoate. Introduction: Benzene contains compounds have special properties that make them to react differently than other molecules. The conjugated compound of dienes in aromatic rings give an extra stability to undergo addition reactions
Premium Nitric acid Nitric acid Benzene
Introduction: The purpose of this lab was to explore the concepts of electrophilic aromatic substitution‚ specifically nitration by synthesizing methyl m-nitrobenzoate using methyl benzoate‚ nitric acid and sulfuric acid. This nitration is a type of electrophilic aromatic substitution. A strongly charged electrophile‚ in this case a nitro group‚ replaces a proton on the aromatic ring. Aromatic compounds undergo substitution instead of addition to maintain resonance. The substituent on the
Premium Benzene Electrophilic aromatic substitution
to the aldol condensation which is followed by elimination. In this reaction E and Z isomers of phenylcinnamic acid are created using benzaldehyde and acetic anhydride. The intermediate product is acetic phenyl acetic anhydride. Benzaldehyde is an aromatic compound that Results: The reaction involves the use of benzaldehyde with acetic anhydride which gave the phenylacetic acid which would be used with in the farther steps of the reaction to produce the E and Z phenylcinnamic acid. The mechanism
Premium Benzene Proton Acetic acid
expected triphenylmethanol. The identity of the product being correct was further confirmed by way of both proton and carbon-13 NMR spectra. This is due to the fact that both spectra yielded peaks that contained the main structural residues‚ alcohol and aromatic groups‚ of triphenylmethanol. The final yield of pure triphenylmethanol was 8.04 grams‚ resulting in a percentage recovery of 67.7%. Introduction When faced with the task of needing to form a new carbon-carbon bond in organic chemistry‚ chemists
Premium Education World War II Learning
Hydrocarbons – Methane (Alkanes) Introduction to Hydrocarbons – Methane Carbons have the ability to form many different compounds. Compounds of carbon that only contain carbon and hydrogen are known as hydrocarbons. Most hydrocarbons naturally occur in the mixture crude oil. There are three main types of hydrocarbons‚ alkanes – saturated hydrocarbons made up of only single bonds‚ alkenes – unsaturated hydrocarbons with one or more double bond/s‚ and alkynes – unsaturated hydrocarbons with one or
Premium Methane Natural gas Petroleum