Insertion and amplification of EGFP protein into pET41a(+) Plasmid Introduction The overall purpose of the experiments conducted is to test the creation of recombinant plasmid using recombinant DNA technology. The research of recombinant DNA began with the use of E.Coli (Escherichia coli)‚ a common bacteria found in the intestines of warm-blooded or- ganisms (5). Scientists worked together and generated a way‚ from cloning and using recombi- nant research‚ to achieve recombinant DNA
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2.MATERIALS and METHODS 2.1. Nature of Reactants 2.1.1 We placed 3mL of 3 M HCl into each of 3 seperate test tubes. Next we added mossy zinc to the first test tubes‚ Sn granules to the second and Cu filings to the third and compared the rates of evolution of hydrogen gas in each test tube. 2.1.2. We mixed 3mL of 0.02 M KMnO4 and 2mL of 3 M H2SO4 in a test tube and stirred using a glass rod. Next‚ we divided the solution into two. We added 2mL of 0.03 M Na2C2O4 solution to the first half and
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tightness in the chest‚ and a cough that lasts over a week. Two symptoms are more prominent in children than in adults. They are blue nails and lips‚ sudden anxiety and apprehension. The last two symptoms to watch out for are when breathing out takes longer than breathing in and when breathing is faster than normal. These symptoms usually occur in episodes and are often called asthma attacks. “When an asthma attack occurs‚ three major changes take place in the lungs. Cells in the air tubes make more
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GREAT DISCOVERIES CHANGE LIVES Do you like watching television? I love it. I do not know what would I do if televisions did not exist. It is interesting‚ in several years‚ they have become a huge part of our lives‚ our indispensables. J.J. Thomson was inspired by some scientists and he provided us the opportunity of having televisions in our lives. Also other scientists improved his thoughts and experiments which leaded to the invention of the television afterwards. It is not that simple of course
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The mother‚ whom the story is about and also the narrator of the story‚ is‚ because of a friend of her son’s recent death‚ recalls on a event which had a huge impact on her life – the death of her own brother. The story revolves around the places in time‚ her childhood and present. The story is told with 2 different stories that in the end become clearer that the 2 stories have a tremendous resemblance. The first story is about the narrator’s childhood‚ where the children of the neighbourhood get
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Complex Ions: 1. Cooper(II) found in this molecular structure has a coordination number of 4 2. Cooper(II) cannot form a crystalline structure with a coordination number of 6. It removes the possibility to create a crystalline structure if the sp3d2 geometry is used. 3. The iron shows a coordination complex of 4 4. The magnesium has a coordination complex of 4 1. A complex ion occurs when we have a metal cation‚ bound by one or many small separate molecules‚ ions‚ or polyatomic
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conscious or unconscious. Still‚ most paintings tend to be similar enough to others in theme or composition that they lend themselves well to being compared and contrasted. I will compare and contrast George Tooker’s The Subway‚ and Cyril E. Power’s The Tube Train. George Tooker’s The Subway is a haunting yet beautiful piece. Painted using the egg tempera method‚ it is stunning for its strategic usage of warm colors. The intention of Tooker’s painting is to portray such a familiar scene as a subway as
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Club Med Case Analysis MGMB01 The Club Med is a successful all-inclusive French organization of vacation resort found in a various sites of the world‚ mostly in exotic areas. Besides obvious financial success‚ the Club also achieved huge non-financial success. The unique concept of “family” ‚ which is also the corporation spirit‚ had transform a group of strangers to a group of loving and caring friends. What’s more‚ they also developed their own set of marketing and distribution strategies.
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would you do differently if you had a chance to do the exercise again? The MedLee: In Pursuit of a joint venture negotiation exercise refers to a joint venture between a US Company and a Family Thai Business Company. I had the role of Pat Armstrong (Med Device Representative: Director of International Strategic Market Research) the issues I had to negotiate were: decision making/control‚ staffing‚ profit distribution and conflict resolution mechanism. The goal of the negotiation was to keep a long-term
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inventions are available we enjoy them and everything but we aren’t shocked because it is the new normal‚ but what if I told you that you could wear an arm band with multiple medications and serums that could cure almost anything. Introducing the Med Band‚ it has everything from snake venom anecdotes to simple ibuprofen‚ when you’re on the go and have a headache you don’t have to worry about finding pills‚ and if you are hiking snake infested terrain you won’t have to worry about getting medical
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