and centrifuged for 20 seconds. A polyacrylamide gel was assembled and the samples were loaded into it. The gel ran for approximately 45 minutes at 160 V. A western blot analysis was done in order to examine the Erk1/2 phosphorylation of the NIH3T3 cells. Three pieces of Whatman paper were wet in transfer buffer and placed on the anode plate. A wet PVDF membrane was placed on top of the Whatman paper. The gel was removed from holder and the stacking gel was removed. Forceps were used to place the gel
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Electrochemical Cells Lab Report AP Chemistry Block 1 Analysis: The purpose of Part 1 of this laboratory is to construct a table listing the reduction potentials of a series of metal ions in order of ease of reduction. The series of half-cells is constructed by placing a piece of metal into a 1.0 M solution of its ions for each metal in the series. The metals are Cu‚ Fe‚ Pb‚ Mg‚ Ag‚ and Zn. The half-cells are connected by a salt bridge constructed of a strip of filter paper soaked in a solution
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Regents Biology 3BCD Osmosis and Plasmolysis Problem: What is the effect of Osmosis on a chicken egg? What is the effect of different solutions with various concentrations on plant cells? Hypothesis: 1. In an animal cell in a hypertonic solution the egg will swell‚ in a hypotonic solution the egg will swell. 2. In a plant cell in a hypertonic solution it will shrink‚ and in a hypotonic solution it will swell. Methodology: Part
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TITLE AND AUTHOR Lab 7 Analysis of purified Concanavalin A via:Hemagglutination INTRODUCTION The purpose of this lab was to test the biological activity of ConA by performing a hemagglutination assay. If ConA is active then agglutination will occur due to ConA’s free receptors being able to bind to the glucose residues on the sheep’s red blood cells. If ConA is not active then no agglutination will occur. To test the hemagglutination reaction‚ two types of ConA solutions
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Plant and animal cells have several differences and similarities. For example‚ animal cells do not have a cell wall or chloroplasts but plant cells do. Animal cells are round and irregular in shape while plant cells have fixed‚ rectangular shapes. Animal Cell Plant Cell Cell wall Absent Present (formed of cellulose) Shape Round (irregular shape) Rectangular (fixed shape) Vacuole One or more small vacuoles (much smaller than plant cells). One‚ large central vacuole taking up 90% of cell volume
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BioLab3 Lab Report 7 Cellular Respiration Answer Key Student Name: I. The ATP Cycle Define the following terms: Autotroph Heterotroph Aerobic respiration Anaerobic respiration Complete the chart below: Letter Defining Term A B C D II. Anaerobic Respiration Define the following terms: Alcoholic fermentation EXERCISE 1 – Alcoholic fermentation At intervals of 20‚ 40‚ and 60 minutes‚ the tubes are removed. Record the
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Differentiating Between Prokaryotic and Eukaryotic Cells‚ Distinguish Among Plant‚ Animal‚ and Protist Cells‚ and Identifying the Organelles that are Evident in Them Introduction There are two different types of cells‚ prokaryotes and eukaryotes. Prokaryotes‚ such as bacteria‚ lack a nuclear membrane and other membrane bound organelles. Their genetic material consists of a single molecule of singular DNA. Eukaryotes‚ such as plant and animal cells‚ have a nuclear membrane and other membrane bound
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observations are repressentative of cell division taking place in the oinon root tip. Firstly‚ this is because our numbers of the number of cells in each phase of mitosis are similar to the rest of the groups‚ so we can assume our results are most likely correct. We can also assume our calculations are right because we know that Interphase is the most active phase in the process of cell divison‚ which would agree with our calculations since we assume that approxiamtly 93% of cells are going though Interphase
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This paper is about how the plant cells and solar cells are similar to each other as well as how they are different to one another. In addition this paper explains how the laws of thermodynamics apply to each of the plant and solar cells system. Plant Cells and Solar Cells Photosynthesis is the process of producing and releasing oxygen in the air. It needs sunlight‚ carbon dioxide and
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Formal lab report: Abstarct: The Purpose of this experiment was to perform Agrobacterium-mediated transformation in Wild type Arabidopsis thaliana Columbia by using somatic plant transformation method. The whole process lasted for over a period of 11 weeks and we were successful in getting transformed plants. Agrobacterium tumefaciens strain containing pMP90 (Ti-helper) plasmid and pCAMBIA1391 (T-DNA) plasmid was used for this plant transformation. pCAMBIA1391 plasmid was constructed by cloning Brassica
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